CAJ | 학술논문

目的比较肿瘤131 I-NGR γ显像及切伦科夫光学显像(CLI)的特征与差异,探讨肿瘤多模态显像的有效方法.方法检测并比较小鼠腹腔注射不同活度(0、740 kBq及7.4 MBq)131I时,体表185 kBq及1.85 MBq131I的γ显像与CLI信号；γ显像及CLI检测不同活度(0、231、463、925和1850 kBq) 131I-NGR与CD13表达阳性的HT1080细胞及表达阴性的HP29细胞的结合情况；对荷瘤裸鼠在尾静脉注射18.5 MBq131I-NGR后2、4、8及12 h进行动态γ显像与CLI,12 h后切除体表肿瘤并移植至腹腔,再次进行γ显像与CLI.结果 γ显像及CLI均能清晰显示体表185 kBq及1.85 MBq的131I;但随腹腔注射131I的增加,γ显像显示的体表131I信号逐步减弱.当腹腔注射7.4 MBq埒131I时,体表185 kBq 131I难以显示,而体表1.85 MBq 131I则仍可显示,但信号明显减低；CLI对体表131I的检测则几乎不受腹腔131I的影响,腹腔注射7.4 MBq 131I仍可清晰显示体表131 I.γ显像及CLI均显示CD13受体表达阳性的HT10180细胞与131 I-NGR特异结合,而阴性表达的HP29细胞不与131I-NGR结合,且HT10180细胞与131I-NGR的结合随131I-NGR的增加而增加.荷瘤裸鼠注射18.5 MBq 131I-NGR后2h CLI可清晰显示肿瘤,12hγ显像显示肿瘤；12 h切除肿瘤移植至腹腔,γ显像清晰显示肿瘤,但CLI却无显示.结论 CLI与γ显像对肿瘤的显像存在差异.CLI对体表肿瘤的早期检测较好,而核素显像对深部肿瘤的检测较好,两者联合的多模态显像有助于提高对肿瘤的检测.
목적 비교종류131 I-NGR γ현상급절륜과부광학현상(CLI)적특정여차이,탐토종류다모태현상적유효방법.방법 검측병비교소서복강주사불동활도(0、740 kBq급7.4 MBq)131I시,체표185 kBq급1.85 MBq131I적γ현상여CLI신호；γ현상급CLI검측불동활도(0、231、463、925화1850 kBq) 131I-NGR여CD13표체양성적HT1080세포급표체음성적HP29세포적결합정황；대하류라서재미정맥주사18.5 MBq131I-NGR후2、4、8급12 h진행동태γ현상여CLI,12 h후절제체표종류병이식지복강,재차진행γ현상여CLI.결과 γ현상급CLI균능청석현시체표185 kBq급1.85 MBq적131I;단수복강주사131I적증가,γ현상현시적체표131I신호축보감약.당복강주사7.4 MBq랄131I시,체표185 kBq 131I난이현시,이체표1.85 MBq 131I칙잉가현시,단신호명현감저；CLI대체표131I적검측칙궤호불수복강131I적영향,복강주사7.4 MBq 131I잉가청석현시체표131 I.γ현상급CLI균현시CD13수체표체양성적HT10180세포여131 I-NGR특이결합,이음성표체적HP29세포불여131I-NGR결합,차HT10180세포여131I-NGR적결합수131I-NGR적증가이증가.하류라서주사18.5 MBq 131I-NGR후2h CLI가청석현시종류,12hγ현상현시종류；12 h절제종류이식지복강,γ현상청석현시종류,단CLI각무현시.결론 CLI여γ현상대종류적현상존재차이.CLI대체표종류적조기검측교호,이핵소현상대심부종류적검측교호,량자연합적다모태현상유조우제고대종류적검측.
Objective To compare the differences and characteristics between Cerenkov luminescence imaging (CLI) and gamma scintigraphy with 131I-NGR in tumor imaging and to evaluate an effective method for imaging tumor by combining both techniques.Methods (1) The 131I sources with radioactivity of 185 kBq and 1.85 MBq were placed at the chest skin surface of 4 mice.CLI and gamma scintigraphy of the surface sources were performed at different peritoneal background by intraperitoneal injection of with different radioactivities (0 kBq,740 kBq and 7.4 MBq).(2) The uptakes of 131 I-NGR under various radioactivities (0,231,463,925,1850 kBq) in CD13-positive HTI080 cells and CD13-negative HP29 cells were evaluated by CLI and gamma scintigraphy.The multi-phase imaging of CLI and gamma scintigraphy for the tumor bearing nude mice was acquired at 2,4,8 and 12 h after injection of 18.5 MBq 131I-NGR.The tumor was resected and implanted at peritoneum for CLI and gamma scintigraphy 12 h post-injection.Results (1) Both CLI and gamma scintigraphy were able to clearly visualize surface sources with radioactivities of 185 kBq and 1.85 MBq.However,the signal on gamma scintigraphy decreased with increased radioactivity in peritoneal cavity.When the peritoneal cavity was injected with 7.4 MBq,the surface source of 185 kBq could not be identified and the source of 1.85 MBq was greatly underestimated.However,Cerenkov signals were less affected by the background activity of peritoneal cavity.Both sources of 185 kBq and 1.85 MBq could be clearly visualized under the background of 7.4 MBq.(2) Both gamma scintigraphy and CLI could show CD13-positive HT1080 cells and were negative for CD13-negative HP29 cells.The higher the dose of 131I-NGR was,the binding with HT1080 cells was more.Subcutaneous tumors could be clearly identified at 2 h by CLI after injection of 18.5 MBq 131I-NGR,but could only be clearly detected by gamma scintigraphy at 12 h.When the tumor was resected and implanted in peritoneal cavity,it could only be detected by gamma scintigraphy.Conclusions CLI is better for detecting surface tumors at its early stage,while gamma scintigraphy is a better choice for the detection of tumor in the body.Combined use of CLI and gamma scintigraphy might improve tumor detection.