中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2014年
1期
48-52
,共5页
张月红%赵长久%吴琼%付鹏%田国梅
張月紅%趙長久%吳瓊%付鵬%田國梅
장월홍%조장구%오경%부붕%전국매
寡核苷酸类,反义%基因扩增%前列腺肿瘤%肿瘤细胞,培养的%放射性核素显像%小鼠,裸
寡覈苷痠類,反義%基因擴增%前列腺腫瘤%腫瘤細胞,培養的%放射性覈素顯像%小鼠,裸
과핵감산류,반의%기인확증%전렬선종류%종류세포,배양적%방사성핵소현상%소서,라
Oligonucleotides,antisense%Gene amplification%Prostatic neoplasms%Tumor cells,cultured%Radionuclide imaging%Mice,nude
目的 探讨99Tcm标记针对小鼠双微体扩增基因(MDM2) mRNA的ASON(MDM2反义探针)用于前列腺癌无创性基因显像的价值.方法 以HYNIC为螯合物,对含MDM2 mRNA某段序列的ASON、错义寡核苷酸(ASONM)进行99Tcm标记,检测标记率及放化纯.建立荷人前列腺癌LNCaP裸鼠肿瘤模型,分为3组(每组10只),进行99Tcm-HYNIC-ASON和99Tcm-HYNIC-ASONM、99TcmO4-(对照)肿瘤显像.测量肿瘤/对侧肢体(T/M)比值,采用单因素方差分析对测量数据进行统计学分析.结果 ASON的99Tcm标记率为(65.15±2.05)％,ASONM的99Tcm标记率为(64.93±2.18)％.99Tcm-HYNIC-ASON和99Tcm-HYNIC-ASONM经纯化后,放化纯均达90％以上.不同探针注射后1、4和10 h时,99Tcm-HYNIC-ASON组T/M比值分别为3.217±0.125、3.749±0.201和4.028±0.186;99Tcm-HYNIC-ASONM组分别为1.579±0.128、1.715±1.140和1.683±0.139;对照组分别为2.146±0.132、1.847±0.124和1.528±0.152.99Tcm-HYNIC-ASON 1、4、10 h T/M比值与对照组和错义组差异均有统计学意义(F=213.37～ 235.41,t=3.527～ 4.738,均P＜0.01),而99Tcm-HYNIC-ASONM组各T/M比值与对照组差异均无统计学意义(t=2.154、2.287和2.236,均P＞0.05).结论 99Tcm标记的MDM2反义探针可在荷人前列腺癌裸鼠模型的肿瘤组织中特异性聚集,可以在早期对前列腺癌进行无创性的基因诊断.
目的 探討99Tcm標記針對小鼠雙微體擴增基因(MDM2) mRNA的ASON(MDM2反義探針)用于前列腺癌無創性基因顯像的價值.方法 以HYNIC為螯閤物,對含MDM2 mRNA某段序列的ASON、錯義寡覈苷痠(ASONM)進行99Tcm標記,檢測標記率及放化純.建立荷人前列腺癌LNCaP裸鼠腫瘤模型,分為3組(每組10隻),進行99Tcm-HYNIC-ASON和99Tcm-HYNIC-ASONM、99TcmO4-(對照)腫瘤顯像.測量腫瘤/對側肢體(T/M)比值,採用單因素方差分析對測量數據進行統計學分析.結果 ASON的99Tcm標記率為(65.15±2.05)％,ASONM的99Tcm標記率為(64.93±2.18)％.99Tcm-HYNIC-ASON和99Tcm-HYNIC-ASONM經純化後,放化純均達90％以上.不同探針註射後1、4和10 h時,99Tcm-HYNIC-ASON組T/M比值分彆為3.217±0.125、3.749±0.201和4.028±0.186;99Tcm-HYNIC-ASONM組分彆為1.579±0.128、1.715±1.140和1.683±0.139;對照組分彆為2.146±0.132、1.847±0.124和1.528±0.152.99Tcm-HYNIC-ASON 1、4、10 h T/M比值與對照組和錯義組差異均有統計學意義(F=213.37～ 235.41,t=3.527～ 4.738,均P＜0.01),而99Tcm-HYNIC-ASONM組各T/M比值與對照組差異均無統計學意義(t=2.154、2.287和2.236,均P＞0.05).結論 99Tcm標記的MDM2反義探針可在荷人前列腺癌裸鼠模型的腫瘤組織中特異性聚集,可以在早期對前列腺癌進行無創性的基因診斷.
목적 탐토99Tcm표기침대소서쌍미체확증기인(MDM2) mRNA적ASON(MDM2반의탐침)용우전렬선암무창성기인현상적개치.방법 이HYNIC위오합물,대함MDM2 mRNA모단서렬적ASON、착의과핵감산(ASONM)진행99Tcm표기,검측표기솔급방화순.건립하인전렬선암LNCaP라서종류모형,분위3조(매조10지),진행99Tcm-HYNIC-ASON화99Tcm-HYNIC-ASONM、99TcmO4-(대조)종류현상.측량종류/대측지체(T/M)비치,채용단인소방차분석대측량수거진행통계학분석.결과 ASON적99Tcm표기솔위(65.15±2.05)％,ASONM적99Tcm표기솔위(64.93±2.18)％.99Tcm-HYNIC-ASON화99Tcm-HYNIC-ASONM경순화후,방화순균체90％이상.불동탐침주사후1、4화10 h시,99Tcm-HYNIC-ASON조T/M비치분별위3.217±0.125、3.749±0.201화4.028±0.186;99Tcm-HYNIC-ASONM조분별위1.579±0.128、1.715±1.140화1.683±0.139;대조조분별위2.146±0.132、1.847±0.124화1.528±0.152.99Tcm-HYNIC-ASON 1、4、10 h T/M비치여대조조화착의조차이균유통계학의의(F=213.37～ 235.41,t=3.527～ 4.738,균P＜0.01),이99Tcm-HYNIC-ASONM조각T/M비치여대조조차이균무통계학의의(t=2.154、2.287화2.236,균P＞0.05).결론 99Tcm표기적MDM2반의탐침가재하인전렬선암라서모형적종류조직중특이성취집,가이재조기대전렬선암진행무창성적기인진단.
Objeetive To explore the value of antisense imaging of 99Tcm-labeled ASON targeting mouse double minute 2(MDM2) mRNA for the diagnosis of human prostate cancer.Methods The ASON targeting MDM2 mRNA and the mismatched oligonucleotide (ASONM) were synthesized and radiolabeled with 99Tcm using the bifunctional chelator HYNIC.The labeling efficiency and radiochemical purity were investigated.Animal models of nude mice bearing human prostate cancer LNCaP were established and divided into 3 groups with 10 mice in each group.99Tcm-HYNIC-ASON,99Tcm-HYNIC-ASONM (study groups) and 99TcmO4-(control group) were injected at the dose of 7.4 MBq through the tail vein,respectively.Tumor imaging was acquired with SPECT and the tumor-to-muscle (T/M) ratio was measured.The data was compared by one-way analysis of variance.Results The labeling efficiencies of ASON and ASONM were (65.15± 2.05) ％ and (64.93±2.18) ％,respectively.Their radiochemical purity was greater than 90％.At 1,4 and 10 h post injection,the T/M ratios of 99Tcm-HYNIC-ASON group were 3.217±0.125,3.749± 0.201 and 4.028±0.186,and those of 99Tcm-HYNIC-ASONM group were 1.579t0.128,1.715±1.140 and 1.683±0.139,and control group 2.146±0.132,1.847±0.124,1.528±0.152,respectively.The T/M ratios in control group and 99Tcm-HYNIC-ASONM group were significantly lower than those in 99Tcm-HYNICASON group at 1,4 and 10 h,respectively (F=213.37-235.41,t=3.527-4.738; all P＜0.01).The T/M ratios of 99Tcm-HYNIC-ASONM group and control group were not significantly different at 1,4 and 10 h (t=2.154,2.287 and 2.236,all P＞0.05).Conclusion The antisense probe of MDM2 can accumulate specifically in prostate cancer tissue in animal models,which might be useful as a non-invasive genetic tool for the early diagnosis of prostate cancer.
