CAJ | 학술논문

纳米抗体作为一种新型抗体片段,保留了完整的抗原结合部位,具有相对分子质量小、特异性高、亲和力强等特性,非常适于构建分子探针用于分子影像.体外实验显示,以纳米抗体为基础的分子影像探针与抗原的亲和力可达到nmol水平.在肿瘤、动脉粥样硬化、神经系统疾病等的动物模型中,纳米抗体分子探针可高特异性地结合到靶器官,未结合的部分由肾脏迅速排泄,具有较高的靶/血液(T/B)比值,可清晰显示病变组织.经结构改造或化学修饰后,纳米抗体与靶器官的亲和力和滞留量进一步提高,更有利于其在分子影像中的应用.随着相关研究的不断深入,以纳米抗体为基础的分子影像在疾病早期诊断及疗效评估中的良好应用前景日渐突显.
납미항체작위일충신형항체편단,보류료완정적항원결합부위,구유상대분자질량소、특이성고、친화력강등특성,비상괄우구건분자탐침용우분자영상.체외실험현시,이납미항체위기출적분자영상탐침여항원적친화력가체도nmol수평.재종류、동맥죽양경화、신경계통질병등적동물모형중,납미항체분자탐침가고특이성지결합도파기관,미결합적부분유신장신속배설,구유교고적파/혈액(T/B)비치,가청석현시병변조직.경결구개조혹화학수식후,납미항체여파기관적친화력화체류량진일보제고,경유리우기재분자영상중적응용.수착상관연구적불단심입,이납미항체위기출적분자영상재질병조기진단급료효평고중적량호응용전경일점돌현.
The nanobody is a novel antibody fragment,which has beneficial biophysical and pharmacokinetic properties,such as the small molecular weight,high affinity and specificity for antigen.Nanobody is ideally suitable for molecular imaging as a targeting probe that could label antigen at nmol level in vitro.In animal models of xenografted tumor,atherosclerotic plaques and brain disorders,the target tissues were specifically and clearly detected and the high tumor-to-blood (T/B) ratios were obtained.Structural or chemical modified nanobodies will have higher affinity and retention to target tissues,and be convenient for the application of molecular imaging.With the development of the related research,nanobody-based molecular imaging will be gradually transformed into the clinical applications,and play an important role in early diagnosis and therapeutic assessment.