中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2014年
3期
231-234
,共4页
毛夕保%章斌%黄景峰%王振欣%张光波
毛夕保%章斌%黃景峰%王振訢%張光波
모석보%장빈%황경봉%왕진흔%장광파
前列腺肿瘤%肿瘤细胞,培养的%转染%B7H3%脱氧葡萄糖%胸苷
前列腺腫瘤%腫瘤細胞,培養的%轉染%B7H3%脫氧葡萄糖%胸苷
전렬선종류%종류세포,배양적%전염%B7H3%탈양포도당%흉감
Prostatic neoplasms%Tumor cells,cultured%Transfection%B7H3%Deoxyglucose%Thymidine
目的 研究转染B7同系物3(B7-H3)基因对前列腺癌细胞摄取18F-FDG和18F-FLT的影响,并探讨抗B7-H3单克隆抗体(简称单抗)对前列腺癌细胞的作用.方法 用细胞计数试剂盒(CCK)-8检测鼠源性前列腺癌RM1细胞和转染B7-H3基因RM1的同种细胞(RM 1-B7-H3)培养后0.5、1、2、3、4和5d的吸光度(A)值,并用流式细胞仪测定2种细胞的生长周期.在不同糖浓度(0、5.5和11.0 mmol/L)、不同细胞数(每孔5×104 ～5×106)、不同摄取时间(20～120 min)的条件下,测定2种细胞的18F-FDG摄取率;并在细胞数为1×106、反应时间为100 min的条件下行18F-FLT细胞摄取实验.最后测定给予抗B7-H3单抗4H7后RM 1-B7-H3细胞的18F-FDG细胞摄取率.数据比较行单因素方差分析和两样本t检验.结果 RM1-B7-H3细胞培养1、2和3d时的A值分别为1.59±0.23、2.26±0.15和2.01±0.60,较RM1细胞(1.22±0.14、1.10±0.09和1.04±0.15)高(t=3.923、19.228和4.467,均P＜0.01);其他时间点2组间差异无统计学意义(t=-0.094、0.858、2.000,均P＞0.05).RM1-B7-H3细胞的G1、S、G2/M期比例分别为(32.96±2.56)％、(39.11±2.57)％和(27.94±0.21)％,S期比例较RM1细胞(32.76±1.90)％高(t=3.442,P＜0.05).2种细胞18F-FDG摄取率均随培养基糖浓度的增加而降低,随细胞数和摄取时间的增加而升高.在1.0× 106细胞、摄取100 min时,RM1-B7-H3和RM1细胞的18F-FDG摄取率分别为(55.07±3.99)％和(44.16±3.60)％,18F-FLT摄取率分别为(5.25±0.81)％和(3.33±0.64)％,差异均有统计学意义(t=4.977和4.567,均P＜0.01).给予4H7单抗后RM1-B7-H3细胞的18F-FDG细胞摄取率为(45.36±2.92)％,较未加单抗组18F-FDG细胞摄取率低(F=10.001,P＜0.01).结论 转染B7-H3基因能增强前列腺癌细胞的代谢和增殖活性,并提高细胞对18F-FDG和18F-FLT的摄取;给予抗B7-H3单抗4H7后,RM1-B7-H3细胞的18F-FDG细胞摄取率降低.
目的 研究轉染B7同繫物3(B7-H3)基因對前列腺癌細胞攝取18F-FDG和18F-FLT的影響,併探討抗B7-H3單剋隆抗體(簡稱單抗)對前列腺癌細胞的作用.方法 用細胞計數試劑盒(CCK)-8檢測鼠源性前列腺癌RM1細胞和轉染B7-H3基因RM1的同種細胞(RM 1-B7-H3)培養後0.5、1、2、3、4和5d的吸光度(A)值,併用流式細胞儀測定2種細胞的生長週期.在不同糖濃度(0、5.5和11.0 mmol/L)、不同細胞數(每孔5×104 ～5×106)、不同攝取時間(20～120 min)的條件下,測定2種細胞的18F-FDG攝取率;併在細胞數為1×106、反應時間為100 min的條件下行18F-FLT細胞攝取實驗.最後測定給予抗B7-H3單抗4H7後RM 1-B7-H3細胞的18F-FDG細胞攝取率.數據比較行單因素方差分析和兩樣本t檢驗.結果 RM1-B7-H3細胞培養1、2和3d時的A值分彆為1.59±0.23、2.26±0.15和2.01±0.60,較RM1細胞(1.22±0.14、1.10±0.09和1.04±0.15)高(t=3.923、19.228和4.467,均P＜0.01);其他時間點2組間差異無統計學意義(t=-0.094、0.858、2.000,均P＞0.05).RM1-B7-H3細胞的G1、S、G2/M期比例分彆為(32.96±2.56)％、(39.11±2.57)％和(27.94±0.21)％,S期比例較RM1細胞(32.76±1.90)％高(t=3.442,P＜0.05).2種細胞18F-FDG攝取率均隨培養基糖濃度的增加而降低,隨細胞數和攝取時間的增加而升高.在1.0× 106細胞、攝取100 min時,RM1-B7-H3和RM1細胞的18F-FDG攝取率分彆為(55.07±3.99)％和(44.16±3.60)％,18F-FLT攝取率分彆為(5.25±0.81)％和(3.33±0.64)％,差異均有統計學意義(t=4.977和4.567,均P＜0.01).給予4H7單抗後RM1-B7-H3細胞的18F-FDG細胞攝取率為(45.36±2.92)％,較未加單抗組18F-FDG細胞攝取率低(F=10.001,P＜0.01).結論 轉染B7-H3基因能增彊前列腺癌細胞的代謝和增殖活性,併提高細胞對18F-FDG和18F-FLT的攝取;給予抗B7-H3單抗4H7後,RM1-B7-H3細胞的18F-FDG細胞攝取率降低.
목적 연구전염B7동계물3(B7-H3)기인대전렬선암세포섭취18F-FDG화18F-FLT적영향,병탐토항B7-H3단극륭항체(간칭단항)대전렬선암세포적작용.방법 용세포계수시제합(CCK)-8검측서원성전렬선암RM1세포화전염B7-H3기인RM1적동충세포(RM 1-B7-H3)배양후0.5、1、2、3、4화5d적흡광도(A)치,병용류식세포의측정2충세포적생장주기.재불동당농도(0、5.5화11.0 mmol/L)、불동세포수(매공5×104 ～5×106)、불동섭취시간(20～120 min)적조건하,측정2충세포적18F-FDG섭취솔;병재세포수위1×106、반응시간위100 min적조건하행18F-FLT세포섭취실험.최후측정급여항B7-H3단항4H7후RM 1-B7-H3세포적18F-FDG세포섭취솔.수거비교행단인소방차분석화량양본t검험.결과 RM1-B7-H3세포배양1、2화3d시적A치분별위1.59±0.23、2.26±0.15화2.01±0.60,교RM1세포(1.22±0.14、1.10±0.09화1.04±0.15)고(t=3.923、19.228화4.467,균P＜0.01);기타시간점2조간차이무통계학의의(t=-0.094、0.858、2.000,균P＞0.05).RM1-B7-H3세포적G1、S、G2/M기비례분별위(32.96±2.56)％、(39.11±2.57)％화(27.94±0.21)％,S기비례교RM1세포(32.76±1.90)％고(t=3.442,P＜0.05).2충세포18F-FDG섭취솔균수배양기당농도적증가이강저,수세포수화섭취시간적증가이승고.재1.0× 106세포、섭취100 min시,RM1-B7-H3화RM1세포적18F-FDG섭취솔분별위(55.07±3.99)％화(44.16±3.60)％,18F-FLT섭취솔분별위(5.25±0.81)％화(3.33±0.64)％,차이균유통계학의의(t=4.977화4.567,균P＜0.01).급여4H7단항후RM1-B7-H3세포적18F-FDG세포섭취솔위(45.36±2.92)％,교미가단항조18F-FDG세포섭취솔저(F=10.001,P＜0.01).결론 전염B7-H3기인능증강전렬선암세포적대사화증식활성,병제고세포대18F-FDG화18F-FLT적섭취;급여항B7-H3단항4H7후,RM1-B7-H3세포적18F-FDG세포섭취솔강저.
Objective To evaluate the effects of B7-H3 gene transfection on 18F-FDG uptake and 18F-FLT uptake in prostate cancer cells.Methods The absorption (A) values of untransfected prostate cancer(RM1) cells and B7-H3 gene-transfected RM1 (RM1-B7-H3) cells were detected at different culturing time points (0.5,1,2,3,4 and 5 d) with cell counting kit-8 (CCK-8) test.Cell cycle phase distribution of RM1 and RM1-B7-H3 cells was measured with flow cytometry.18F-FDG uptake of RM1 and RM1-B7-H3 cells was measured with γcounter and calculated under different conditions:5× 104-5× 106 cells; 0-11.0 mmol/L glucose; 20-120 min incubation in 37 ℃.18F-FLT uptake of RM1 and RM1-B7-H3 cells was measured in 1×106 cells under incubation for 100 min at 37 ℃.After administering anti-B7-H3 monoclonal antibody 4H7,18F-FDG uptake of RM1-B7-H3 cells was measured.The data were analyzed using one-way analysis of variance and two-sample t test.Results The A values of RM1-B7-H3 cells after being incubated for 1,2 and 3 d were higher than those of RM1 cells(1.59±0.23,2.26±0.15 and 2.01±0.60 vs 1.22±0.14,1.10± 0.09 and 1.04±0.15,t=3.923,19.228,4.467,all P＜0.01).There was no statistical significance between the 2 groups at other time points (t=-0.094,0.858,2.000,all P＞0.05).The ratios of RM1-B7-H3 cells in G1,S and G2/M phases were(32.96±2.56) ％,(39.11 ±2.57) ％ and (27.94±0.21) ％,respectively.The ratio of S phase in RM1-B7-H3 cells was higher than that in RM1 cells ((32.76±1.90)％,t=3.442,P＜ 0.05).18F-FDG uptake of the both cell lines decreased with the increase of glucose concentrations,while the uptake went up with the increase of cell number and incubation time.With the cell number of 1.0× 106,incubation time of 100 min and temperature of 37 ℃,the 18F-FDG uptake of RM1-B7-H3 and RM1 cells was (55.07±3.99)％ vs (44.16±3.60)％ (t=4.977,P＜0.01) ; and 18F-FLT uptake of RM1-B7-H3 and RM1 cells was (5.25±0.81)％ vs (3.33±0.64)％ (t=4.567,P＜0.01).After treated with antibody 4H7,18F-FDG uptake of RM1-B7-H3 cells ((45.36±2.92) ％) was lower than that of untreated group (F=10.001,P＜ 0.01).Conclusion B7-H3 gene transfection may promote the metabolism and proliferation of prostate cancer cells,and thereby increase the 18F-FDG uptake and 18F-FLT uptake.
