中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2014年
5期
385-389
,共5页
张金山%罗良平%史长征%李园%安薇
張金山%囉良平%史長徵%李園%安薇
장금산%라량평%사장정%리완%안미
肺肿瘤%肿瘤移植%细胞凋亡%放射疗法%碘放射性同位素%磁共振成像%膜联蛋白V
肺腫瘤%腫瘤移植%細胞凋亡%放射療法%碘放射性同位素%磁共振成像%膜聯蛋白V
폐종류%종류이식%세포조망%방사요법%전방사성동위소%자공진성상%막련단백V
Lung neoplasms%Neoplasm transplantation%Cell apoptosis%Radiotherapy%Iodine radioisotopes%Magnetic resonance imaging%Annexin V
目的 探讨99Tcm-膜联蛋白V(Annexin V)显像联合磁共振弥散加权成像(MR-DWI)非创伤性活体检测125I粒子植入内照射致肺腺癌裸鼠移植瘤细胞凋亡的可行性.方法 将25只A549肺腺癌细胞皮下种植的BALB/c-nu裸鼠模型按随机数字表法分为实验组(n=13)和对照组(n=12).实验组进行125I粒子[表观活度为(24.8±6.3)MBq/粒]植入,对照组进行无活度的“冷粒子”植入,7～10d后进行99Tcm-Annexin V细胞凋亡显像,并同期行MR-DWI,然后处死裸鼠,取瘤体标本进行原位末端标记法(TUNEL)荧光免疫细胞凋亡检测及Survivin免疫组织化学检测.采用两独立样本t检验、x2检验和Pearson相关分析进行数据分析.结果 (1)实验组99Tcm-Annexin V细胞凋亡显像阳性率为69.2％(9/13),大于对照组的8.3％(1/12,x2=12.73,P＜0.01);(2)实验组瘤体99Tcm-Annexin V摄取比值、表观弥散系数(ADC)和细胞凋亡指数(AI)分别为2.91±0.85、(2.03±0.44)×10-3 mm2/s和(49±18)％,对照组相应指标分别为1.26±0.37,(1.29±0.21)×10-3 mm2/s和(11±4)％,实验组较对照组高(t=5.930、5.452和7.606,均P＜0.05),而对照组的Survivin阳性表达率高于实验组[(46±13)％和(15±7)％,t=5.158,P＜0.05];(3)ADC、AI和瘤体摄取比值间两两相关(r分别为0.756、0.788和0.754,均P＜0.05),Survivin表达与AI呈负相关(r=-0.772,P＜0.05).结论 Survivin表达下调致细胞凋亡可能是125I内照射治疗A549肺腺癌裸鼠移植瘤的机制之一;99Tcm-Annexin V显像联合MR-DWI能有效地对移植瘤细胞凋亡情况进行非创伤性活体检测,有利于125I粒子内照射治疗早期疗效的判断.
目的 探討99Tcm-膜聯蛋白V(Annexin V)顯像聯閤磁共振瀰散加權成像(MR-DWI)非創傷性活體檢測125I粒子植入內照射緻肺腺癌裸鼠移植瘤細胞凋亡的可行性.方法 將25隻A549肺腺癌細胞皮下種植的BALB/c-nu裸鼠模型按隨機數字錶法分為實驗組(n=13)和對照組(n=12).實驗組進行125I粒子[錶觀活度為(24.8±6.3)MBq/粒]植入,對照組進行無活度的“冷粒子”植入,7～10d後進行99Tcm-Annexin V細胞凋亡顯像,併同期行MR-DWI,然後處死裸鼠,取瘤體標本進行原位末耑標記法(TUNEL)熒光免疫細胞凋亡檢測及Survivin免疫組織化學檢測.採用兩獨立樣本t檢驗、x2檢驗和Pearson相關分析進行數據分析.結果 (1)實驗組99Tcm-Annexin V細胞凋亡顯像暘性率為69.2％(9/13),大于對照組的8.3％(1/12,x2=12.73,P＜0.01);(2)實驗組瘤體99Tcm-Annexin V攝取比值、錶觀瀰散繫數(ADC)和細胞凋亡指數(AI)分彆為2.91±0.85、(2.03±0.44)×10-3 mm2/s和(49±18)％,對照組相應指標分彆為1.26±0.37,(1.29±0.21)×10-3 mm2/s和(11±4)％,實驗組較對照組高(t=5.930、5.452和7.606,均P＜0.05),而對照組的Survivin暘性錶達率高于實驗組[(46±13)％和(15±7)％,t=5.158,P＜0.05];(3)ADC、AI和瘤體攝取比值間兩兩相關(r分彆為0.756、0.788和0.754,均P＜0.05),Survivin錶達與AI呈負相關(r=-0.772,P＜0.05).結論 Survivin錶達下調緻細胞凋亡可能是125I內照射治療A549肺腺癌裸鼠移植瘤的機製之一;99Tcm-Annexin V顯像聯閤MR-DWI能有效地對移植瘤細胞凋亡情況進行非創傷性活體檢測,有利于125I粒子內照射治療早期療效的判斷.
목적 탐토99Tcm-막련단백V(Annexin V)현상연합자공진미산가권성상(MR-DWI)비창상성활체검측125I입자식입내조사치폐선암라서이식류세포조망적가행성.방법 장25지A549폐선암세포피하충식적BALB/c-nu라서모형안수궤수자표법분위실험조(n=13)화대조조(n=12).실험조진행125I입자[표관활도위(24.8±6.3)MBq/립]식입,대조조진행무활도적“랭입자”식입,7～10d후진행99Tcm-Annexin V세포조망현상,병동기행MR-DWI,연후처사라서,취류체표본진행원위말단표기법(TUNEL)형광면역세포조망검측급Survivin면역조직화학검측.채용량독립양본t검험、x2검험화Pearson상관분석진행수거분석.결과 (1)실험조99Tcm-Annexin V세포조망현상양성솔위69.2％(9/13),대우대조조적8.3％(1/12,x2=12.73,P＜0.01);(2)실험조류체99Tcm-Annexin V섭취비치、표관미산계수(ADC)화세포조망지수(AI)분별위2.91±0.85、(2.03±0.44)×10-3 mm2/s화(49±18)％,대조조상응지표분별위1.26±0.37,(1.29±0.21)×10-3 mm2/s화(11±4)％,실험조교대조조고(t=5.930、5.452화7.606,균P＜0.05),이대조조적Survivin양성표체솔고우실험조[(46±13)％화(15±7)％,t=5.158,P＜0.05];(3)ADC、AI화류체섭취비치간량량상관(r분별위0.756、0.788화0.754,균P＜0.05),Survivin표체여AI정부상관(r=-0.772,P＜0.05).결론 Survivin표체하조치세포조망가능시125I내조사치료A549폐선암라서이식류적궤제지일;99Tcm-Annexin V현상연합MR-DWI능유효지대이식류세포조망정황진행비창상성활체검측,유리우125I입자내조사치료조기료효적판단.
Objective To detect apoptosis of transplanted lung adenocarcinoma cells in nude mice after 125I brachytherapy by 99Tcm-Annexin V combined with diffusion weighted MRI (MR-DWI).Methods Twenty-five BALB/c-nu nude mice models subcutaneously transplanted with A549 cells were divided into experimental group (EG,n=13) and control group (CG,n=12) by random number table method.One 125I seed with apparent activity of (24.8±6.3) MBq was implanted into each mouse in EG,while CG underwent cold seed implantation.Both of 99Tcm-Annexin V imaging and MR-DWI were performed within 7-10 d after brachytherapy,then all mice were sacrificed and tumor cell apoptosis was detected by terminal oxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) immunofluorescence,Survivin expression was assayed by SP.Two-sample t test,x2 test and Pearson correlation analysis were used for data analysis.Results Positive rate of cell apoptosis by 99Tcm-Annexin V imaging was 69.2％(9/13) and 8.3％(1/12) respectively in EG and CG (x2 =12.73,P＜0.01).The uptake ratio of 99Tcm-Annexin V,apparent diffusion coefficient(ADC) value and apoptosis index(M) of the tumor in EG were 2.91±0.85,(2.03±0.44)×10-3 mm2/s and (49± 18) ％,respectively,which were significantly higher than those of CG (1.26 ± 0.37,(1.29 ± 0.21) ×10-3 mm2/s and (11±4)％ respectively,t=5.930,5.452,7.606,all P＜0.05).Survivin expression in EG and CG was (46± 13) ％ and (15±7) ％ respectively (t =5.158,P＜0.05).The value of ADC was correlated with AI and uptake ratio(r=0.756,0.788,both P＜0.05).Uptake ratio was correlated with AI (r=0.754,P＜0.05),while Survivin expression was negatively correlated with AI (r =-0.772,P＜0.05).Conclusions Down-regulation of Survivin expression may play an important role in apoptosis induced by 125I brachytherapy.99Tcm-Annexin V combined with MR-DWI could effectively evaluate apoptosis of lung adenocarcinoma cells in a non-invasive way,thus it might be helpful in evaluation of early efficacy of 125I brachytherapy.
