CAJ | 학술논문

目的建立人气道肉芽组织成纤维细胞的原代培养方法,为进一步研究良性气道狭窄的病理机制奠定基础.方法收集2011年4-6月北京天坛医院呼吸科气管镜术中收集的6例气道狭窄患者气管内增生肉芽组织,其中男2例,女4例.使用组织块法培养人气道肉芽组织成纤维细胞,于倒置显微镜下观察细胞生长情况并照相.结果 6例人气道肉芽组织成纤维细胞的原代培养均成功,培养组织块贴壁后在第3～4天可见有典型长梭形细胞爬出.培养至9～11d,可见大量长梭形细胞在组织块周围呈放射状排列,此时可进行细胞传代.传代后细胞生长良好,形成单层后可再次传代.结论组织块培养法是进行人气道肉芽组织成纤维细胞原代培养的可行方法.
목적 건립인기도육아조직성섬유세포적원대배양방법,위진일보연구량성기도협착적병리궤제전정기출.방법 수집2011년4-6월북경천단의원호흡과기관경술중수집적6례기도협착환자기관내증생육아조직,기중남2례,녀4례.사용조직괴법배양인기도육아조직성섬유세포,우도치현미경하관찰세포생장정황병조상.결과 6례인기도육아조직성섬유세포적원대배양균성공,배양조직괴첩벽후재제3～4천가견유전형장사형세포파출.배양지9～11d,가견대량장사형세포재조직괴주위정방사상배렬,차시가진행세포전대.전대후세포생장량호,형성단층후가재차전대.결론 조직괴배양법시진행인기도육아조직성섬유세포원대배양적가행방법.
Objective To establish a feasible method to culture primary fibroblasts isolated from human airway granulation tissues,and therefore to provide experimental data for the investigation of the pathogenesis of benign airway stenosis.Methods The granulation tissues were collected from 6 patients during routine bronchoscopy at our department of Beijing Tiantan Hospital from April to June 2011.Primary fibroblasts were obtained by culturing the explanted tissues.Cell growth was observed under inverted microscope.Results All of these 6 primary cultures were successful.Fibroblast-like cells were observed to migrate from the tissue pieces 3 d after inoculation.After 9-11 d of culture,cells reached to 90％ confluence and could be sub-cultured.After passage,the cells were still in a typical elongated spindle-shape and grew well.The cells could be sub-cultured further when they formed a monolayer.Conclusion Explant culture is a reliable method for culturing primary fibroblasts from human airway granulation tissues.