CAJ | 학술논문

目的评估以噻吩二羧酸肼(TCH)实验结果作为鉴定牛分枝杆菌主要依据的可靠性.方法使用对硝基苯甲酸和TCH培养基对4069株分枝杆菌临床分离株进行鉴定,并将对MTB复合群中对5 mg/L的TCH敏感菌株再次接种至含2 mg/L的TCH罗氏培养基中进行敏感度测试,同时采用间隔区寡核苷酸分型技术和多位点PCR技术对TCH敏感菌株进行鉴定.结果 4069株分枝杆菌临床分离株中有3929株属于MTB复合群菌株,3929株中对5 mg/L的TCH实验敏感菌株为245株,245株中对2mg/L TCH仍敏感的菌株为20株,间隔区寡核苷酸分型技术和多位点PCR技术鉴定出245株分离株中仅有1株为牛分枝杆菌.结论 TCH实验浓度不论是5 mg/L还是2 mg/L,均难以成为鉴定牛分枝杆菌的可靠依据.
목적 평고이새분이최산정(TCH)실험결과작위감정우분지간균주요의거적가고성.방법 사용대초기분갑산화TCH배양기대4069주분지간균림상분리주진행감정,병장대MTB복합군중대5 mg/L적TCH민감균주재차접충지함2 mg/L적TCH라씨배양기중진행민감도측시,동시채용간격구과핵감산분형기술화다위점PCR기술대TCH민감균주진행감정.결과 4069주분지간균림상분리주중유3929주속우MTB복합군균주,3929주중대5 mg/L적TCH실험민감균주위245주,245주중대2mg/L TCH잉민감적균주위20주,간격구과핵감산분형기술화다위점PCR기술감정출245주분리주중부유1주위우분지간균.결론 TCH실험농도불론시5 mg/L환시2 mg/L,균난이성위감정우분지간균적가고의거.
Objective To evaluate the reliability of thiophen-2-carboxylic acid hydrazine (TCH) test for identification of Mycobacterium bovis (M.bovis).Methods A total of 4069 clinically isolated strains were identified by P-Nitrobenzoic acid medium (500 mg/L) and TCH medium (5 mg/L).Mycobacterium tuberculosis (M.tuberculosis) complex strains susceptible to 5 mg/L TCH were further tested for susceptibility to 2 mg/L TCH.Spacer oligonucleotide typing (spoligotyping) and multi-loci PCR were also performed to identify TCH susceptible strains.Results Among the 4069 isolated strains there were 3929 strains belonging to M.tuberculosis complex (MTBC) of which 245 were susceptible to 5 mg/L TCH.Of these 245 strains,20 were also susceptible to 2 mg/L TCH,while only 1 strain was identified to be M.bovis by both spoligotyping and multi-loci PCR.Conclusion TCH susceptibility test (either 5 mg/L or 2 mg/L) is not a reliable method for identification of M.bovis.