CAJ | 학술논문

目的探讨间歇低氧对舌下神经电活性长时程易化(LTF)的影响.方法 12只成年SD大鼠采用随机数字表法分为慢性间歇低氧组(实验组)及常氧对照组(对照组),每组6只,实验组大鼠放置于间歇低氧动物仓中,对照组放置于常氧动物仓中,每天8h(晨8时至16时),连续4周,随后给予急性间歇低氧刺激,每次5 min,共3次,记录两组大鼠急性间歇低氧前后舌下神经放电活性.结果实验组和对照组基础状态下放电频率分别为(73±13)和(58±11)Hz,放电幅度分别为(4.6±1.1)和(3.3±0.7)μV,差异均有统计学意义(均P＜0.05).经急性间歇低氧干预后,实验组及对照组大鼠舌下神经的放电频率及电活性幅度均明显升高(均P＜0.05).这种放电水平的增强可持续＞1h,呈典型的长时程易化.实验组在急性低氧处理前、后的放电频率分别为(68±16)和(133±20)Hz,电活性幅度分别为(4.6±1.1)和(8.9±1.4)μV;而对照组在急性间歇低氧处理前、后的放电频率分别为(59±12)和(102±16)Hz,电活性幅度分别为(3.2±0.7)和(4.5±0.7)μV.实验组大鼠经急性间歇低氧干预后呼吸强度的增加幅度明显高于对照组,分别为(408±149)％和(242±31)％,差异有统计学意义(P＜0.05).结论急性及慢性间歇低氧均可诱导舌下神经长时程易化,而慢性间歇低氧可增强急性间歇低氧诱导的舌下神经长时程易化.
목적 탐토간헐저양대설하신경전활성장시정역화(LTF)적영향.방법 12지성년SD대서채용수궤수자표법분위만성간헐저양조(실험조)급상양대조조(대조조),매조6지,실험조대서방치우간헐저양동물창중,대조조방치우상양동물창중,매천8h(신8시지16시),련속4주,수후급여급성간헐저양자격,매차5 min,공3차,기록량조대서급성간헐저양전후설하신경방전활성.결과 실험조화대조조기출상태하방전빈솔분별위(73±13)화(58±11)Hz,방전폭도분별위(4.6±1.1)화(3.3±0.7)μV,차이균유통계학의의(균P＜0.05).경급성간헐저양간예후,실험조급대조조대서설하신경적방전빈솔급전활성폭도균명현승고(균P＜0.05).저충방전수평적증강가지속＞1h,정전형적장시정역화.실험조재급성저양처리전、후적방전빈솔분별위(68±16)화(133±20)Hz,전활성폭도분별위(4.6±1.1)화(8.9±1.4)μV;이대조조재급성간헐저양처리전、후적방전빈솔분별위(59±12)화(102±16)Hz,전활성폭도분별위(3.2±0.7)화(4.5±0.7)μV.실험조대서경급성간헐저양간예후호흡강도적증가폭도명현고우대조조,분별위(408±149)％화(242±31)％,차이유통계학의의(P＜0.05).결론 급성급만성간헐저양균가유도설하신경장시정역화,이만성간헐저양가증강급성간헐저양유도적설하신경장시정역화.
Objective To explore the impact of intermittent hypoxia on long-term facilitation (LTF) of hypoglossal nerve discharge.Methods Twelve adult SD rats were divided into the experimental group (CIH group,n =6) and the control group (normoxia group,n =6) by the random number table.The rats in the CIH group were fed in the intermittent hypoxia animal chambers,while the control group was placed in the normoxia animal chambers for 8 h per day (from 8:00 AM to 4:00 PM) for 4 consecutive weeks.After that,5 min× 3 stimulations of acute intermittent hypoxia (AIH) were administered and the hypoglossal never signals were recorded before and after AIH.Results The baseline frequency and average peak amplitude of hypoglossal nerve discharge in the CIH experimental group were significantly greater than those in the control group.The discharge frequency in the CIH and the control groups was (73 ± 13) Hz,and (58 ± 11) Hz,respectively(P ＜0.05) ; and the discharge amplitude in the 2 groups was (4.6 ± 1.1) μV,and (3.3 ± 0.7) μV,respectively (P ＜ 0.05).After intervention with AIH,the frequency and the average peak amplitude of the hypoglossal nerve discharge in the experimental and the control groups were significantly increased (all P ＜ 0.05).The increased discharge lasted more than 1 h and this typical phenomenon was referred to as LTF.In the CIH group,the discharge frequency before and after exposure to AIH was (68 ± 16) Hz and (133 ±20) Hz,respectively;and the discharge amplitude was (4.6 ± 1.1) μV and (8.9 ± 1.4) μV,respectively.In the control group,the discharge frequency before and after AIH was (59 ± 12) Hz and (102± 16) Hz,respectively; and the discharge amplitude was (3.3 ±0.7) μV and (4.5 ±0.7) μV,respectively(P ＜0.05).After AIH stimulation,the enhanced respiratory intensity of rats in CIH group was much higher than that in the control group [(408 ± 149)％ vs (242 ±31)％,P ＜0.05].Conclusion Both AIH and CIH can induce LTF of the hypoglossal nerve discharge,while the induction of LTF by AIH can be strengthened by CIH.