CAJ | 학술논문

目的探讨去乙酰化酶沉默信息调节因子2相关酶1(SIRT1)与大鼠睡眠呼吸暂停指数的相关性及不同缺氧模型对大鼠低位脑干SIRT1表达量的影响.方法对健康SD大鼠33只进行睡眠呼吸监测,随后取其低位脑干组织,应用Western blot对低位脑干组织SIRT1相对表达量进行检测,分析大鼠睡眠呼吸暂停指数与SIRT1表达量的相关性.另取健康SD大鼠36只按随机数字表法分为4组:对照组、持续缺氧组(CH组)、间歇缺氧组(IH组)和间歇缺氧伴高二氧化碳组(IHH组),每组9只,使用Western blot法检测其低位脑干组织SIRT1相对表达量,比较4组大鼠SIRT1表达量的差异.结果 SIRT1与快动眼睡眠期自发性睡眠呼吸暂停指数(SPAI)呈负相关(r=-0.617,P＜0.01),与REM期叹息后睡眠呼吸暂停指数(PSAI)呈正相关(r=0.535,P＜0.01).对照组、CH组、IH组、IHH组脑干SIRT1蛋白相对表达量分别为1.4±1.0、1.4±0.6、2.5±1.0和1.6±1.2,差异有统计学意义(F=2.964,P＜0.05).结论脑干SIRT1表达量低的大鼠可能因抗氧化应激能力低,中枢调控不稳,导致其自发呼吸暂停增多.缺氧情况下SIRT1表达升高,推测是一种保护机制.
목적 탐토거을선화매침묵신식조절인자2상관매1(SIRT1)여대서수면호흡잠정지수적상관성급불동결양모형대대서저위뇌간SIRT1표체량적영향.방법 대건강SD대서33지진행수면호흡감측,수후취기저위뇌간조직,응용Western blot대저위뇌간조직SIRT1상대표체량진행검측,분석대서수면호흡잠정지수여SIRT1표체량적상관성.령취건강SD대서36지안수궤수자표법분위4조:대조조、지속결양조(CH조)、간헐결양조(IH조)화간헐결양반고이양화탄조(IHH조),매조9지,사용Western blot법검측기저위뇌간조직SIRT1상대표체량,비교4조대서SIRT1표체량적차이.결과 SIRT1여쾌동안수면기자발성수면호흡잠정지수(SPAI)정부상관(r=-0.617,P＜0.01),여REM기우식후수면호흡잠정지수(PSAI)정정상관(r=0.535,P＜0.01).대조조、CH조、IH조、IHH조뇌간SIRT1단백상대표체량분별위1.4±1.0、1.4±0.6、2.5±1.0화1.6±1.2,차이유통계학의의(F=2.964,P＜0.05).결론 뇌간SIRT1표체량저적대서가능인항양화응격능력저,중추조공불은,도치기자발호흡잠정증다.결양정황하SIRT1표체승고,추측시일충보호궤제.
Objective To explore the correlation between SIRT1 expression in brainstem and apnea hypopnea index and the effect of hypoxia on SIRT1 expression in rats.Methods Thirty-three healthy Sprague-Dawley(SD) rats underwent sleep monitoring and SIRT1 protein in brainstems was measured by Western blot.The correlation of sleep apnea index and SIRT1 protein level in brainstem was analysed.Another 36 adult male SD rats were randomly and equally divided into 4 groups:normal control (NC)group,continuous hypoxia (CH) group,intermittent hypoxia (IH) group and intermittent hypoxia with hypercapnia (IHH) group.The expression of SIRT1 protein of the lower brainstems of the 4 groups were measured by Western blot.Results The level of SIRT1 in the brainstem was negtively correlated with the frequency of spontaneous sleep apnea in REM (r =-0.617,P ＜ 0.001) while positively correlated with the frequency of post sigh sleep apnea in REM (r =0.535,P ＜ 0.01).The relative expression level of SIRT1 in NC,CH,IH,IHHgroupwere(1.4±1.0),(1.4±0.6),(2.5±1.0),(1.6±1.2),respectively.There was a significant difference in the SIRT1 level among groups (F =2.964,P ＜ 0.05).Conclusions The rats with lower level of SIRT1 may have lower anti-oxidative stress capability which results in higher frequency of spontaneous sleep apnea.Under the circumstance of hypoxia,the fact that the expression of SIRT1 protein was elevated may be a protective mechanism.