CAJ | 학술논문

目的探讨急性应激抑郁模型大鼠大脑中缝核、海马、前额叶、纹状体及外周血5-羟色胺1A(5-HT1A)与5-羟色胺2A(5-HT2A)受体mRNA表达的变化.方法将雄性SD大鼠分为实验组和对照组,每组8只；实验组给予连续3 d电击诱导急性应激抑郁模型.采用旷场实验评定其行为学改变；应用逆转录聚合酶链反应检测并比较2组大鼠大脑中缝核、海马、前额叶、纹状体及外周血粒细胞5-HT1A与5-HT2A受体mRNA的表达水平.结果 (1)实验组5-HT2A受体mR NA表达在海马、中缝核、前额叶、纹状体分别为0.35±0.29、0.22±0.16、0.79 ±0.33、0.24 ±0.18,对照组分别为2.00±2 14、1.05 ±0.88、1.64±1.51、1.26±0.75,2组在海马、中缝核、纹状体的差异有统计学意义(P均＜0.05).(2)实验组5-HT1A受体mRNA表达在海马、中缝核、前额叶、纹状体分别为2.02±1.91、0.87±1.54、1.74±1.45、1.52±1.56,对照组分别为0.99±0.63、0.97±1.34、1.05±1.04、2.48±3.82,2组比较差异无统计学意义(P＞0.05).(3)实验组外周血粒细胞5-HT1A、5-HT2A受体mRNA表达分别为1.55 ±0.75和0.47±0.47,对照组分别为1.13±0.34和1.31 ±1.20,2组比较差异无统计学意义(P＞0.05).结论急性应激大鼠大脑5-HT2A受体mRNA表达在中缝核、海马、纹状体减少,在前额叶、外周血无变化；5-HT1A受体mRNA表达在上述脑区及外周血中均无变化.
목적 탐토급성응격억욱모형대서대뇌중봉핵、해마、전액협、문상체급외주혈5-간색알1A(5-HT1A)여5-간색알2A(5-HT2A)수체mRNA표체적변화.방법 장웅성SD대서분위실험조화대조조,매조8지；실험조급여련속3 d전격유도급성응격억욱모형.채용광장실험평정기행위학개변；응용역전록취합매련반응검측병비교2조대서대뇌중봉핵、해마、전액협、문상체급외주혈립세포5-HT1A여5-HT2A수체mRNA적표체수평.결과 (1)실험조5-HT2A수체mR NA표체재해마、중봉핵、전액협、문상체분별위0.35±0.29、0.22±0.16、0.79 ±0.33、0.24 ±0.18,대조조분별위2.00±2 14、1.05 ±0.88、1.64±1.51、1.26±0.75,2조재해마、중봉핵、문상체적차이유통계학의의(P균＜0.05).(2)실험조5-HT1A수체mRNA표체재해마、중봉핵、전액협、문상체분별위2.02±1.91、0.87±1.54、1.74±1.45、1.52±1.56,대조조분별위0.99±0.63、0.97±1.34、1.05±1.04、2.48±3.82,2조비교차이무통계학의의(P＞0.05).(3)실험조외주혈립세포5-HT1A、5-HT2A수체mRNA표체분별위1.55 ±0.75화0.47±0.47,대조조분별위1.13±0.34화1.31 ±1.20,2조비교차이무통계학의의(P＞0.05).결론 급성응격대서대뇌5-HT2A수체mRNA표체재중봉핵、해마、문상체감소,재전액협、외주혈무변화；5-HT1A수체mRNA표체재상술뇌구급외주혈중균무변화.
Objective To investigate the message ribonucleic acid (mRNA) expression of 5-HT1A receptor and 5-HT2A receptor in raphe nuclei,hippocampi,prefrontal cortex,striatum and blood of acute stressed depression rat model.Methods All 16 male SD rats were divided into 2 groups:control and experimental group.The experimental group was made by foot shock once a day last for 3 days.Open field was used to measure rats' behavior.The mRNA expressions of 5-HT1A and 5-HT2A receptors in raphe nuclei,hippocampus,prefrontal cortex,striatum and blood were assayed with real-time reverse-transcription polymerase chain reaction (PCR).Results (1) Compared to control,mRNA expression of S-HT2A receptor reduced in hippocampi(0.35 ±0.29 vs.2.00 ± 2.14; P ＜0.05),raphe nuclei(0.22 ±0.16 vs.1.05 ±0.88; P ＜ 0.05)and striatum(0.24 ± 0.18 vs.1.26 ± 0.75; P ＜ 0.01).(2) Compared to control,mRNA expression of 5-HT1A receptor in hippocampi(2.02 ± 1.91 vs.0.99 ±0.63),raphe nuclei(0.87 ± 1.54 vs.0.97 ± 1.34),prefrntal cortex(1.74 ± 1.45 vs.1.05 ± 1.04),striatum(1.52 ± 1.56 vs.2.48 ± 3.82) of acute stressed depression rat model was not significantly different (All P ＞ 0.05).(3) The blood mRNA expression of both 5-HT1A receptor(1.55 ± 0.75 vs.1.13 ±0.34)and 5-HT2A receptor(0.47 ±0.47 vs.1.31± 1.20)were not significantly different between control and experimental group(All P ＞ 0.05).Conclusion The mRNA expression of 5-HT2A receptor may be decreased in hippocampi,raphe nuclei and striatum of acute stressed depression rat model,while expression of 5-HT1A receptor might not change.