CAJ | 학술논문

目的探讨生命早期母爱剥夺对成年雄性大鼠行为和海马神经再生的影响.方法 24只雄性大鼠按随机数字表法分为母爱剥夺组(12只)和对照组(12只).母爱剥夺组大鼠出生后第2～14天每天接受3h母爱剥夺,90～100日龄时采用糖水偏好测验和旷场测验评估大鼠的快感缺乏、焦虑水平和探索能力,BrdU标记并采用免疫组化方法检测大鼠海马齿状回的再生神经细胞.结果 (1)母爱剥夺组的糖水偏好[(61.0±5.6)％]、旷场总行程[(3065±895)cm]、内围场地行程[(199±287) cm]、直立次数[(10.0±4.8)次]均少于对照组[分别为(80.9±3.5)％,(4888±1112) cm,(483±324) cm,(14.9±5.2)次],2组比较差异有统计学意义(t=-10.398,P=0.000；t=-4.425,P =0.000；t=-2.275,P=0.033；t=-2.415,P=0.024).(2)母爱剥夺组海马齿状回BrdU阳性细胞数[(20.2±7.1)个]少于对照组[(32.8±8.7)个],差异有统计学意义(t=-3.900,P=0.001).结论生命早期应激会改变成年大鼠的行为和抑制海马齿状回神经再生,可能会导致成年后抑郁易感性增加.
목적 탐토생명조기모애박탈대성년웅성대서행위화해마신경재생적영향.방법 24지웅성대서안수궤수자표법분위모애박탈조(12지)화대조조(12지).모애박탈조대서출생후제2～14천매천접수3h모애박탈,90～100일령시채용당수편호측험화광장측험평고대서적쾌감결핍、초필수평화탐색능력,BrdU표기병채용면역조화방법검측대서해마치상회적재생신경세포.결과 (1)모애박탈조적당수편호[(61.0±5.6)％]、광장총행정[(3065±895)cm]、내위장지행정[(199±287) cm]、직립차수[(10.0±4.8)차]균소우대조조[분별위(80.9±3.5)％,(4888±1112) cm,(483±324) cm,(14.9±5.2)차],2조비교차이유통계학의의(t=-10.398,P=0.000；t=-4.425,P =0.000；t=-2.275,P=0.033；t=-2.415,P=0.024).(2)모애박탈조해마치상회BrdU양성세포수[(20.2±7.1)개]소우대조조[(32.8±8.7)개],차이유통계학의의(t=-3.900,P=0.001).결론 생명조기응격회개변성년대서적행위화억제해마치상회신경재생,가능회도치성년후억욱역감성증가.
Objective To explore the effects of maternal deprivation on behavior and neurogenesis in hippocampal dentate gyrus in adult rats.Methods Twenty-four newborn male rats were randomly divided into two groups,maternal deprivation group and control group.The maternal deprivation group were deprived from their mother 3 hours per day during postnatal day 2 to 14.The anhedonia,spontaneous anxiety level and the exploratory ability in novel environments was assessed with sucrose preference test and open-field test in aduhhood(at the age of 90-100 days).Then,rats were sacrificed and hippocampus was isolated.Newly generated cells in hippocampal dentate gyrus were labeled by BrdU and detected by using immunohistochemistry method.Results (1) The rate of sucrose preference [(61.0 ± 5.6) ％],total distance[(3065 ± 895) cm],central total distance [(199 ± 287) cm] and the frequency of erection [(10.0 ±4.8)times] in maternal deprivation group were significant less than that in the control group [(80.9±3.5)％,(4888 ± 1112) cm,(483 ±324) cm and (14.9 ±5.2)times respectively(t =-10.398,P=0.000;t =-4.425,P =0.000;t=-2.275,P=0.033;t=-2.415,P =0.024)].(2) BrdU-labeled cells in the dentate gyrus in maternal deprivation group (20.2 ± 7.1) were significant less than that in control group(32.8 ± 8.7 ;t =-3.900,P =0.001).Conclusions Early life stress could alter behavior and neurogenesis in hippocampal dentate gyrus in adult male rats.It may increase susceptibility to depressive disorders.