CAJ | 학술논문

目的评价盐酸米诺环素软膏对钛片表面成骨细胞生长的影响,以期为盐酸米诺环素软膏应用于种植体周围炎的治疗提供依据.方法将Ⅳ级商业纯钛棒制备成光滑、酸蚀、喷砂酸蚀3种不同表面形态的钛片,分别为光滑组、酸蚀组及喷砂酸蚀组,每组41片,表面涂布盐酸米诺环素软膏1周,经纯净水荡洗后分别用扫描电镜观察涂药前后3组钛片表面形态结构的改变,用表面粗糙度测量仪测量涂药前后钛片表面粗糙度值,配对t检验分析粗糙度的变化；用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法检测涂抹盐酸米诺环素软膏及未涂药的3组钛片与人成骨肉瘤细胞(MG-63细胞)培养1、4和7d时的吸光度值(A值),双因素方差分析各组细胞在不同钛片上的生长增殖差异.结果 3组钛片经盐酸米诺环素软膏处理前后的表面粗糙度分别为:光滑组(0.093±0.025)、(0.086±0.026) μm；酸蚀组(1.100±0.095)、(1.009±0.196) μm；喷砂酸蚀组(2.837±0.283)、(2.968±0.206)μm,各组涂药前后表面粗糙度差异均无统计学意义(P值分别为0.118、0.436、0.692)；扫描电镜观察结果显示3组钛片经盐酸米诺环素软膏处理前后表面形态一致,酸蚀组和喷砂酸蚀组钛片涂药后表面可见药物残留；MTT法检测MG-63细胞在钛片表面的生长在培养1、4和7d时3种表面处理组之间,以及同组涂药和未涂药钛片之间差异均无统计学意义(P＞0.05).结论在本项研究的范围内,盐酸米诺环素软膏处理未对钛片的表面形态及粗糙度产生明显影响；MG-63细胞能够在盐酸米诺环素软膏处理并经纯水超声荡洗后的钛片上黏附和增殖.
목적 평개염산미낙배소연고대태편표면성골세포생장적영향,이기위염산미낙배소연고응용우충식체주위염적치료제공의거.방법 장Ⅳ급상업순태봉제비성광활、산식、분사산식3충불동표면형태적태편,분별위광활조、산식조급분사산식조,매조41편,표면도포염산미낙배소연고1주,경순정수탕세후분별용소묘전경관찰도약전후3조태편표면형태결구적개변,용표면조조도측량의측량도약전후태편표면조조도치,배대t검험분석조조도적변화；용갑기새서기사서(methyl thiazolyl tetrazolium,MTT)법검측도말염산미낙배소연고급미도약적3조태편여인성골육류세포(MG-63세포)배양1、4화7d시적흡광도치(A치),쌍인소방차분석각조세포재불동태편상적생장증식차이.결과 3조태편경염산미낙배소연고처리전후적표면조조도분별위:광활조(0.093±0.025)、(0.086±0.026) μm；산식조(1.100±0.095)、(1.009±0.196) μm；분사산식조(2.837±0.283)、(2.968±0.206)μm,각조도약전후표면조조도차이균무통계학의의(P치분별위0.118、0.436、0.692)；소묘전경관찰결과현시3조태편경염산미낙배소연고처리전후표면형태일치,산식조화분사산식조태편도약후표면가견약물잔류；MTT법검측MG-63세포재태편표면적생장재배양1、4화7d시3충표면처리조지간,이급동조도약화미도약태편지간차이균무통계학의의(P＞0.05).결론 재본항연구적범위내,염산미낙배소연고처리미대태편적표면형태급조조도산생명현영향；MG-63세포능구재염산미낙배소연고처리병경순수초성탕세후적태편상점부화증식.
Objective To investigate the effect of minocycline hydrochloride ointment on cell attachment and proliferation on titanium disks.Methods Commercially pure (grade 4) machined titanium discs with three different kinds of surfaces (smooth,acid-etched and sandblasted combined with acid-etched)were treated with minocycline ointment for 1 week,and then cleaned in ultrasonic cleanser for 10 minutes.Surface properties were examined by scanning electron microscope (SEM) and roughness tester before and after the treatment. Surface roughness was compared by paired t test. MG-63 (human osteoblast-like osteosarcoma cell) cells were seeded on these three kinds of discs with or without minocycline treatment,and methl thiazolyl tetrazolium (MTT) was performed to investigate the attachment in the 1 st day and proliferation in the 4 th and 7 th day. Data were analyzed by double factor analysis of variance. Results Surface roughness before and after minocycline application was as follows,Smooth:( 0.093 ± 0.025 ) μm,(0.086 ± 0.026) μm; Acid-etched:( 1.100 ± 0.095 ) μm,( 1.009 ± 0.196) μm ; Sandblasted combined with acid-etched:(2.837 ±0.283) μm,(2.968 ±0.206) μm.No significant changes in roughness were found before and after minocycline application ( P values were 0.118,0.436 and 0.692).SEM examination revealed as similar surface configuration after minocycline application as before,except for some remnant of the minocycline ointment in acid-etched and sandblasted combined acid-etched groups. In MTT test,the growth of MG-63 cells in the 1 st,4 th day and 7 th day was not different between groups with and without minocycline application( P values were 0.450,0.848 and 0.835 ),and among three groups of different surface(P values were 0.184,0.579 and 0.331 ).Conclusions Minocycline hydrochloride ointment did not affect the surface configuration,surface roughness or the properties for cell attachment and proliferation of titanium discs.