中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
z1期
16-20
,共5页
侵袭性牙周炎%慢性牙周炎%年龄因素%DKK-1
侵襲性牙週炎%慢性牙週炎%年齡因素%DKK-1
침습성아주염%만성아주염%년령인소%DKK-1
Aggressive periodontitis%Chronic periodontitis%Age factors%DKK-1
目的 观察牙周炎与血清Dickkopf-1(DKK-1)水平的关系,探讨影响血清DKK-1水平的可能因素.方法 收集侵袭性牙周炎(aggressive periodontitis,AgP)、慢性牙周炎(chronic periodontitis,CP)患者各20例(分别为AgP组、CP组)及分别与AgP、CP患者年龄匹配的健康对照者各20名(分别为H1组、H2组),采用酶联免疫吸附测定法检测血清DKK-1水平.采用t检验比较各组血清中DKK-1水平的差异,并采用相关分析和多元线性回归方法分析血清DKK-1水平与牙周临床参数及年龄等因素的关系,检测水准为双侧α =0.05.结果 AgP组血清DKK-1水平[(12.36±3.19) μg/L]显著高于CP组[(8.90 ±2.73) μg/L] (P=0.001),但与其对照组H1组[(12.37±2.74) μg/L]相比差异无统计学意义(P=0.99),CP组与H2组[(8.85 ±2.56) μg/L]相比差异也无统计学意义(P =0.896)；AgP与CP组血清DKK-1水平与牙周探诊深度、附着丧失、出血指数、探诊出血阳性位点百分比间均未见显著相关(AgP组r值分别为-0.029、-0.223、0.062、-0.412;CP组r值分别为-0.156、0.185、-0.379、0.051)；总体样本血清DKK-1水平与年龄呈显著负相关(r=-0.453,P =0.000)；多元线性回归分析显示年龄对血清DKK-1水平有影响(β=-0.391,t=-3.626,P=0.001).结论 未发现牙周炎对血清DKK-1水平产生影响；年龄是影响血清DKK-1水平的重要因素,血清DKK-1水平随年龄增加而降低.
目的 觀察牙週炎與血清Dickkopf-1(DKK-1)水平的關繫,探討影響血清DKK-1水平的可能因素.方法 收集侵襲性牙週炎(aggressive periodontitis,AgP)、慢性牙週炎(chronic periodontitis,CP)患者各20例(分彆為AgP組、CP組)及分彆與AgP、CP患者年齡匹配的健康對照者各20名(分彆為H1組、H2組),採用酶聯免疫吸附測定法檢測血清DKK-1水平.採用t檢驗比較各組血清中DKK-1水平的差異,併採用相關分析和多元線性迴歸方法分析血清DKK-1水平與牙週臨床參數及年齡等因素的關繫,檢測水準為雙側α =0.05.結果 AgP組血清DKK-1水平[(12.36±3.19) μg/L]顯著高于CP組[(8.90 ±2.73) μg/L] (P=0.001),但與其對照組H1組[(12.37±2.74) μg/L]相比差異無統計學意義(P=0.99),CP組與H2組[(8.85 ±2.56) μg/L]相比差異也無統計學意義(P =0.896)；AgP與CP組血清DKK-1水平與牙週探診深度、附著喪失、齣血指數、探診齣血暘性位點百分比間均未見顯著相關(AgP組r值分彆為-0.029、-0.223、0.062、-0.412;CP組r值分彆為-0.156、0.185、-0.379、0.051)；總體樣本血清DKK-1水平與年齡呈顯著負相關(r=-0.453,P =0.000)；多元線性迴歸分析顯示年齡對血清DKK-1水平有影響(β=-0.391,t=-3.626,P=0.001).結論 未髮現牙週炎對血清DKK-1水平產生影響；年齡是影響血清DKK-1水平的重要因素,血清DKK-1水平隨年齡增加而降低.
목적 관찰아주염여혈청Dickkopf-1(DKK-1)수평적관계,탐토영향혈청DKK-1수평적가능인소.방법 수집침습성아주염(aggressive periodontitis,AgP)、만성아주염(chronic periodontitis,CP)환자각20례(분별위AgP조、CP조)급분별여AgP、CP환자년령필배적건강대조자각20명(분별위H1조、H2조),채용매련면역흡부측정법검측혈청DKK-1수평.채용t검험비교각조혈청중DKK-1수평적차이,병채용상관분석화다원선성회귀방법분석혈청DKK-1수평여아주림상삼수급년령등인소적관계,검측수준위쌍측α =0.05.결과 AgP조혈청DKK-1수평[(12.36±3.19) μg/L]현저고우CP조[(8.90 ±2.73) μg/L] (P=0.001),단여기대조조H1조[(12.37±2.74) μg/L]상비차이무통계학의의(P=0.99),CP조여H2조[(8.85 ±2.56) μg/L]상비차이야무통계학의의(P =0.896)；AgP여CP조혈청DKK-1수평여아주탐진심도、부착상실、출혈지수、탐진출혈양성위점백분비간균미견현저상관(AgP조r치분별위-0.029、-0.223、0.062、-0.412;CP조r치분별위-0.156、0.185、-0.379、0.051)；총체양본혈청DKK-1수평여년령정현저부상관(r=-0.453,P =0.000)；다원선성회귀분석현시년령대혈청DKK-1수평유영향(β=-0.391,t=-3.626,P=0.001).결론 미발현아주염대혈청DKK-1수평산생영향；년령시영향혈청DKK-1수평적중요인소,혈청DKK-1수평수년령증가이강저.
Objective To determine whether Dickkopf-1 (DKK-1)expression could be associated with periodontitis.Methods Fourty periodontitis patients and 40 age-matched healthy subjects as control were enrolled in this study.Periodontitis patients included 20 aggressive periodontitis (AgP) and 20 chronic periodontitis(CP).Control subjects included 20 age matched for AgP and 20 age matched for CP respectively.Clinical examination consisted of probing depth (PD),attachment loss (AL),bleeding index (BI),and percentage of sites with bleeding on probing(BOP).Serum DKK-1 levels were determined using an enzyme-linked immunosorbent assay(ELISA).Comparisons of serum DKK-1 levels between groups were performed using the t-test.Spearman correlation analyses were conducted between serum DKK-1 levels and age,gender,smoker respectively.Multiple linear regression analysis was performed between serum DKK-1 levels and age,gender,smoker,PD,AL,BI,BOP,two-tailed α =0.05.Results The concentrations of serum DKK-1 in AgP patients [(12.36 ± 3.19) μg/L] were significantly higher than those in CP patients [(8.90 ± 2.73) μg/L] (P =0.001).However,no significant difference of serum DKK-1 levels was found between AgP group and its age-matched healthy control [(12.37 ± 2.74) μg/L] (P =0.99),nor thatbetween CP group and its age-matched healthy control[(8.85 ±2.56) μg/L] (P =0.896).No correlations between the serum DKK-1 levels and the periodontal parameters (PD、AL、BI、BOP) were detected (in AgP group,r =-0.029,-0.223,0.062,-0.412 respectively ; in CP group,r =-0.156,0.185,-0.379,0.051 respectively).Combine AgP patients,CP patients and their age-matched healthy subjects together,serum DKK-1 levels in all the samples were found to be negative correlation with age (r =-0.453,P ＜0.001).Multiple linear regression analysis showed that serum DKK-1 levels were negative correlation with age(β =-0.391,t =-3.626,P =0.001).Conclusions No association between periodontitis and serum DKK-1 levels was found.Age has impact on the serum levels of DKK-1.The concentration of serum DKK-1 levels may decrease with age.
