CAJ | 학술논문

目的了解牙周炎及糖尿病易感性与脂联素基因45位点和276位点多态性的关系,以期为进一步预防糖尿病伴慢性牙周炎的发生提供依据.方法选取2008年1月至2010年12月四川大学华西口腔医学院就诊者、四川大学华西医院健康检查中心的体检者及四川大学华西口腔医学院病源库的患者共200例,按全身及牙周情况分为健康对照组、慢性牙周炎组、2型糖尿病组、慢性牙周炎伴2型糖尿病组,每组50例.分别收集颊黏膜拭子,提取DNA,再通过聚合酶链反应-限制性片段长度多态性检测脂联素基因45位点和276位点的基因型分布.对结果数据计量资料行方差分析,计数资料行x2检验,对性别、年龄、276位点和45位点基因多态性与慢性牙周炎和2型糖尿病的关系进行多因素Logistic回归分析,检验水准为双侧α=0.05.结果健康对照组276位点基因型分布(GG:20例,GT:26例,TT:4例)与2型糖尿病组(GG:16例,GT:20例,TT:14例)差异有统计学意义(P=0.034)；45位点基因型分布在健康对照组(GG:26例,TG:21例,TT:3例)与2型糖尿病组(GG:27例,TG:22例,TT:1例)差异无统计学意义(P =0.856)；健康对照组276位点基因型分布与慢性牙周炎组(GG:14例,GT:30例,TT:6例)差异无统计学意义(P =0.418)；45位点基因型在健康对照组及慢性牙周炎组(GG:30例,TG:18例,TT:2例)差异亦无统计学意义(P =0.699).多项Logistic回归分析表明仅在慢性牙周炎伴2型糖尿病组中性别与慢性牙周炎和2型糖尿病的共同发病有关(P=0.011,OR =2.938).结论本项研究结果表明276位点基因多态性与2型糖尿病间存在着密切联系,而45位点基因多态性则与2型糖尿病无关；两者均与慢性牙周炎无关.
목적 료해아주염급당뇨병역감성여지련소기인45위점화276위점다태성적관계,이기위진일보예방당뇨병반만성아주염적발생제공의거.방법 선취2008년1월지2010년12월사천대학화서구강의학원취진자、사천대학화서의원건강검사중심적체검자급사천대학화서구강의학원병원고적환자공200례,안전신급아주정황분위건강대조조、만성아주염조、2형당뇨병조、만성아주염반2형당뇨병조,매조50례.분별수집협점막식자,제취DNA,재통과취합매련반응-한제성편단장도다태성검측지련소기인45위점화276위점적기인형분포.대결과수거계량자료행방차분석,계수자료행x2검험,대성별、년령、276위점화45위점기인다태성여만성아주염화2형당뇨병적관계진행다인소Logistic회귀분석,검험수준위쌍측α=0.05.결과 건강대조조276위점기인형분포(GG:20례,GT:26례,TT:4례)여2형당뇨병조(GG:16례,GT:20례,TT:14례)차이유통계학의의(P=0.034)；45위점기인형분포재건강대조조(GG:26례,TG:21례,TT:3례)여2형당뇨병조(GG:27례,TG:22례,TT:1례)차이무통계학의의(P =0.856)；건강대조조276위점기인형분포여만성아주염조(GG:14례,GT:30례,TT:6례)차이무통계학의의(P =0.418)；45위점기인형재건강대조조급만성아주염조(GG:30례,TG:18례,TT:2례)차이역무통계학의의(P =0.699).다항Logistic회귀분석표명부재만성아주염반2형당뇨병조중성별여만성아주염화2형당뇨병적공동발병유관(P=0.011,OR =2.938).결론 본항연구결과표명276위점기인다태성여2형당뇨병간존재착밀절련계,이45위점기인다태성칙여2형당뇨병무관；량자균여만성아주염무관.
Objective To study the relationship between adiponectin gene polymorphisms and the susceptibility of the chronic periodontitis and diabetes mellitus.Methods Two hundreds patients were selected and divided into four groups,according to their physical and periodontal status.The four group were as follows,the healthy group as control,the one with chronic periodontitis,the one with type Ⅱ diabetes mellitus,and the one suffered from the both.Then,DNA was extracted from buccal mucosa swabs,and the genotype of single nucleotide polymorphism (SNP) 276 as well as SNP 45 in adiponectin gene can be obtained using polymerase chain reaction (PCR) together with restrictive fragment length polymorphism analysis.The relationship between the adiponectin gene polymorphism and chronic periodontitis and diabetes mellitus was analyzed statistically.Results There was significant difference between the normal healthy group (GG:20,GT:26,TT:4) and the diabetes mellitus group (GG:16,GT:20,TT:14) at SNP 276 (P =0.034).But there is no statistically difference between the normal healthy group (GG:26,TG:21,TT:3) and the diabetes mellitus group (GG:27,TG:22,TT:1) at SNP 45 (P =0.856).Moreover,the distribution of SNP 276 shows no difference between the normal healthy group aud the group with chronic periodontitis (GG:14,GT:30,TT:6) (P =0.418).No significant difference was found from the adiponectin haplotype analysis of SNP 45 between the normal healthy group and the group with chronic periodontitis (GG:30,TG:18,TT:2) (P =0.699).According to Logistic regression,the OR of sex is 2.938 in the chronic periodontitis group with the mellitus diabetes(P =0.011).Conclusions These results suggest that SNP 276 in the ADIPOQ gene is associated with diabetes mellitus type 2,but not with chronic periodontitis.There was no association between the adiponectin gene 45 polymorphism and diabetes mellitus type 2.Besides,neither of SNP 276 and 45 has somethiug to do with chronic periodontitis.