中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
z1期
74-76
,共3页
包广洁%高秋爽%黄双盛%郭曼丽
包廣潔%高鞦爽%黃雙盛%郭曼麗
포엄길%고추상%황쌍성%곽만려
蜂胶%链球菌,变异%微生物敏感性试验%厚朴酚
蜂膠%鏈毬菌,變異%微生物敏感性試驗%厚樸酚
봉효%련구균,변이%미생물민감성시험%후박분
Propolis%Streptococcus mutans%Microbial sensitivity tests%Magnolol
目的 观察蜂胶与厚朴酚混合物对变形链球菌的生长抑制作用,以期为进一步开发利用复方中药防龋提供参考.方法 根据蜂胶、厚朴酚及二者混合液的最小抑菌浓度选择实验所需的质量浓度分别为:蜂胶250、125、62.5、31.25、15.625 g/L(蜂胶组),厚朴酚0.0625、0.0313、0.0156、0.0078、0.0039g/L(厚朴酚组),蜂胶与厚朴酚混合液A1(250 g/L蜂胶+0.0625 g/L厚朴酚)、A2(125g/L蜂胶+0.0313 g/L厚朴酚)、A3(62.5 g/L蜂胶+0.0156 g/L厚朴酚)、A4 (31.25 g/L蜂胶+0.0078 g/L厚朴酚)、A5(15.6 g/L蜂胶+0.0039 g/L厚朴酚)(混合组),另设0.95％乙醇组和菌液组作为对照,将蜂胶、厚朴酚及两者混合物分别倍比稀释至各浓度,采用纸片法观察各组对变形链球菌生长的影响.结果 蜂胶及厚朴酚组分别在质量浓度为62.5、0.0156 g/L时才出现明显的抑菌环,而混合组在蜂胶及厚朴酚最小质量浓度A5时即出现了明显的抑菌环；蜂胶组、厚朴酚组及混合组的抑菌作用均随质量浓度的增大而增强,3组分别在最小质量浓度时的抑菌环直径为(0.000±0.000)、(0.000±0.000)、(0.770±0.085) mm,在最大质量浓度时的抑菌环直径为(1.515±0.017)、(1.837±0.283)、(2.853±0.229) mm,且5种质量浓度下混合组的抑菌环直径均显著大于蜂胶组和厚朴酚组(P＜0.05).结论 蜂胶与厚朴酚混合物抑菌效果较二者单独作用时明显增强,提示复方中药防龋存在很大的开发潜力.
目的 觀察蜂膠與厚樸酚混閤物對變形鏈毬菌的生長抑製作用,以期為進一步開髮利用複方中藥防齲提供參攷.方法 根據蜂膠、厚樸酚及二者混閤液的最小抑菌濃度選擇實驗所需的質量濃度分彆為:蜂膠250、125、62.5、31.25、15.625 g/L(蜂膠組),厚樸酚0.0625、0.0313、0.0156、0.0078、0.0039g/L(厚樸酚組),蜂膠與厚樸酚混閤液A1(250 g/L蜂膠+0.0625 g/L厚樸酚)、A2(125g/L蜂膠+0.0313 g/L厚樸酚)、A3(62.5 g/L蜂膠+0.0156 g/L厚樸酚)、A4 (31.25 g/L蜂膠+0.0078 g/L厚樸酚)、A5(15.6 g/L蜂膠+0.0039 g/L厚樸酚)(混閤組),另設0.95％乙醇組和菌液組作為對照,將蜂膠、厚樸酚及兩者混閤物分彆倍比稀釋至各濃度,採用紙片法觀察各組對變形鏈毬菌生長的影響.結果 蜂膠及厚樸酚組分彆在質量濃度為62.5、0.0156 g/L時纔齣現明顯的抑菌環,而混閤組在蜂膠及厚樸酚最小質量濃度A5時即齣現瞭明顯的抑菌環；蜂膠組、厚樸酚組及混閤組的抑菌作用均隨質量濃度的增大而增彊,3組分彆在最小質量濃度時的抑菌環直徑為(0.000±0.000)、(0.000±0.000)、(0.770±0.085) mm,在最大質量濃度時的抑菌環直徑為(1.515±0.017)、(1.837±0.283)、(2.853±0.229) mm,且5種質量濃度下混閤組的抑菌環直徑均顯著大于蜂膠組和厚樸酚組(P＜0.05).結論 蜂膠與厚樸酚混閤物抑菌效果較二者單獨作用時明顯增彊,提示複方中藥防齲存在很大的開髮潛力.
목적 관찰봉효여후박분혼합물대변형련구균적생장억제작용,이기위진일보개발이용복방중약방우제공삼고.방법 근거봉효、후박분급이자혼합액적최소억균농도선택실험소수적질량농도분별위:봉효250、125、62.5、31.25、15.625 g/L(봉효조),후박분0.0625、0.0313、0.0156、0.0078、0.0039g/L(후박분조),봉효여후박분혼합액A1(250 g/L봉효+0.0625 g/L후박분)、A2(125g/L봉효+0.0313 g/L후박분)、A3(62.5 g/L봉효+0.0156 g/L후박분)、A4 (31.25 g/L봉효+0.0078 g/L후박분)、A5(15.6 g/L봉효+0.0039 g/L후박분)(혼합조),령설0.95％을순조화균액조작위대조,장봉효、후박분급량자혼합물분별배비희석지각농도,채용지편법관찰각조대변형련구균생장적영향.결과 봉효급후박분조분별재질량농도위62.5、0.0156 g/L시재출현명현적억균배,이혼합조재봉효급후박분최소질량농도A5시즉출현료명현적억균배；봉효조、후박분조급혼합조적억균작용균수질량농도적증대이증강,3조분별재최소질량농도시적억균배직경위(0.000±0.000)、(0.000±0.000)、(0.770±0.085) mm,재최대질량농도시적억균배직경위(1.515±0.017)、(1.837±0.283)、(2.853±0.229) mm,차5충질량농도하혼합조적억균배직경균현저대우봉효조화후박분조(P＜0.05).결론 봉효여후박분혼합물억균효과교이자단독작용시명현증강,제시복방중약방우존재흔대적개발잠력.
Objective To study the effects of the composite of propolis and magnolol on the growth of Streptococcus mutans (Sm).Methods The solution concentration of the propolis (250,125,62.5,31.25,15.625 g/L),magnolol (0.0625,0.0313,0.0156,0.0078,0.0039 g/L) and the composite of those two (propolis,magnolol) A1 (250 g/L,0.0625 g/L),A2(125 g/L,0.0313 g/L),A3 (62.5 g/L,0.0156 g/L),A4 (31.25 g/L,0.0078 g/L),A5 (15.625 g/L,0.0039 g/L) were chosen according to pretested minimum inhibitory concentration (MIC).The solutions were proportionally diluted to the presupposed concentration before operated on the Sm.Meanwhile,the solvent and the bacterial were also operated on the Sm as controls.Slip diffusion method was adopted to measure the inhibitory effect of those solutions on Sm.Results Bacteria inhibition zones were appeared at the concentration of 62.5 g/L prololis and 0.0156 g/L magnolol respectively.A inhibition zone appeared at the lower concentration of A5 (15.625 g/L,0.0039 g/L) of the propolis and magnolol composite.As general,the inhibition effects increased with concentration.The difference were statistically significant,P ＜ 0.05.Conclusions The inhibitory effect of the composite of propolis and magnolol would be much stronger than either of those two separately.
