CAJ | 학술논문

目的分析口腔黏膜病初诊患者唾液真菌培养标本中红酵母属的检出率、病原学及分离人群特征,探讨其在口腔中的可能作用.方法收集2010年1月至2011年12月间北京大学口腔医学院·口腔医院中医黏膜科唾液真菌培养阳性病例,分析红酵母菌的检出状况.30株菌落数＞ 200 CFU/ml的红酵母菌经API 20C AUX系统和内转录间隔区基因测序鉴定到种,行体外药敏试验,并回顾性分析分离人群的临床特征.结果 2010年和201 1年红酵母属的检出率(占全年唾液培养阳性标本的百分比)分别为2.5％(78/3113)和2.9％(82/2823)；30株红酵母菌中2株为黏红酵母菌,28株为胶红酵母菌,对唑类药物抑制90％受试菌时的最小抑菌质量浓度为氟康唑＞128 mg/L;伊曲康唑＞4 mg/L;伏立康唑=4 mg/L.携带红酵母属的病例以口干[30％(9/30)]和高血糖患者[30％(9/30)]居多.结论红酵母属可分离自口腔黏膜病患者唾液,对唑类药物敏感性较差,其携带可能与口腔及全身状况相关.
목적 분석구강점막병초진환자타액진균배양표본중홍효모속적검출솔、병원학급분리인군특정,탐토기재구강중적가능작용.방법 수집2010년1월지2011년12월간북경대학구강의학원·구강의원중의점막과타액진균배양양성병례,분석홍효모균적검출상황.30주균락수＞ 200 CFU/ml적홍효모균경API 20C AUX계통화내전록간격구기인측서감정도충,행체외약민시험,병회고성분석분리인군적림상특정.결과 2010년화201 1년홍효모속적검출솔(점전년타액배양양성표본적백분비)분별위2.5％(78/3113)화2.9％(82/2823)；30주홍효모균중2주위점홍효모균,28주위효홍효모균,대서류약물억제90％수시균시적최소억균질량농도위불강서＞128 mg/L;이곡강서＞4 mg/L;복립강서=4 mg/L.휴대홍효모속적병례이구간[30％(9/30)]화고혈당환자[30％(9/30)]거다.결론 홍효모속가분리자구강점막병환자타액,대서류약물민감성교차,기휴대가능여구강급전신상황상관.
Objective To explore the possible role of oral carriage of Rhodotorula spp (Rho) by preliminarily investigating the detection rate in oral mucosal disease patients,microbiological characteristics,population features and in vitro antifungal drug susceptibility.Methods The positive saliva fungi culture cases were collected during Jan 2010 to Dec 2011 and the detection ratio of Rho from the positive fungal culture was analyzed.Thirty strains with CFU ＞ 200 per ml were collected and identified.The conventional API 20 C AUX kit supplemented by sequencing the ITS region of each strain was used in the identification of each strain.Additionally,the in vitro antifungal susceptibility tests were performed by using ATB FUNGUS 3 kit according to CLSI M27-A2 document.Clinical data of 30 cases carrying the isolated stains were analyzed retrospectively.Results The Rho ratio in 2010 and 2011 was 2.5％(78/3113)and 2.9％(82/2823)respectively.Twenty-eight strains were identified as Rhodotorula mucilaginosa,and the other 2 strains were identified as Rhodotorula glutinis.The result of in vitro antifungal susceptibility test of Rho revealed rather high MIC90 value to three azole drugs (fluconazole ＞ 128 ; itraconazole ＞ 4 ; voriconazole =4).Nine of the 30 cases(30％) were with Sjogren syndrome/dry mouth conditions,and nine of the 30 cases(30％) were with diabetes mellitus/hyperglycemia.Conclusions Oral Rho can be isolated from patients with oral mucosal diseases.The drug susceptibility to azoles was low.Oral carriage of Rho might be associated with oral diseases and systemic disorders.