中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
z1期
123-126
,共4页
沈雪敏%薛婷君%施琳俊%刘伟%周曾同
瀋雪敏%薛婷君%施琳俊%劉偉%週曾同
침설민%설정군%시림준%류위%주증동
白色念珠菌%细胞周期%小檗碱%流式细胞术
白色唸珠菌%細胞週期%小檗堿%流式細胞術
백색념주균%세포주기%소벽감%류식세포술
Candida albicans%Cell cycle%Berberine%Flow cytometry
目的 应用流式细胞仪测定小檗碱对白色念珠菌细胞周期的影响,初步探索小檗碱抑制白色念珠菌的作用机制.方法 将白色念珠菌(ATCC76615)培养在含不同质量浓度(0.16、0.08、0.04及0 g/L)小檗碱的酵母浸出粉胨葡萄糖培养基中,培养6、12和24h后应用流式细胞仪检测该药物对白色念珠菌(ATCC76615)细胞生长周期的影响.结果 白色念珠菌(ATCC76615)在与小檗碱作用12h时,随着药物质量浓度的增高(0、0.04、0.08、0.16 g/L)增殖指数(分别为98.8、82.4、75.6、54.1)均显著降低(P＜0.001).结论 小檗碱对白色念珠菌的细胞生长周期具有抑制作用,其最佳作用时间为12h.
目的 應用流式細胞儀測定小檗堿對白色唸珠菌細胞週期的影響,初步探索小檗堿抑製白色唸珠菌的作用機製.方法 將白色唸珠菌(ATCC76615)培養在含不同質量濃度(0.16、0.08、0.04及0 g/L)小檗堿的酵母浸齣粉胨葡萄糖培養基中,培養6、12和24h後應用流式細胞儀檢測該藥物對白色唸珠菌(ATCC76615)細胞生長週期的影響.結果 白色唸珠菌(ATCC76615)在與小檗堿作用12h時,隨著藥物質量濃度的增高(0、0.04、0.08、0.16 g/L)增殖指數(分彆為98.8、82.4、75.6、54.1)均顯著降低(P＜0.001).結論 小檗堿對白色唸珠菌的細胞生長週期具有抑製作用,其最佳作用時間為12h.
목적 응용류식세포의측정소벽감대백색념주균세포주기적영향,초보탐색소벽감억제백색념주균적작용궤제.방법 장백색념주균(ATCC76615)배양재함불동질량농도(0.16、0.08、0.04급0 g/L)소벽감적효모침출분동포도당배양기중,배양6、12화24h후응용류식세포의검측해약물대백색념주균(ATCC76615)세포생장주기적영향.결과 백색념주균(ATCC76615)재여소벽감작용12h시,수착약물질량농도적증고(0、0.04、0.08、0.16 g/L)증식지수(분별위98.8、82.4、75.6、54.1)균현저강저(P＜0.001).결론 소벽감대백색념주균적세포생장주기구유억제작용,기최가작용시간위12h.
Objective To determine the effect of berberine on cell cycle of Candida albicans.Methods Flow cytometry (FCM) was used to determine the effect of berberine on cell cycle of Candida albicans.After Candida albicans (ATCC76615) was incubated in yeast extract peptone dextrose (YPD)medium which contained different concentration of berberine for 6 hours,12 hours and 48 hours respectively,the cell cycle was detected by FCM.The proliferation index(PI) was calculated.Results PI was significantly inhibited when Candida albicans (ATCC76615)was incubated with berberine for 12 hours (0 g/L:98.8,0.04 g/L:82.4,0.08 g/L:75.6,0.16 g/L:54.1).Conclusions The berberine could inhibit the cell cycle of Candida albicans in vitro,wbich may be the potential mechanism of antifungal effect of berberine against Candida albicans.
