中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
z1期
149-152
,共4页
李小彤%杨雁琪%杜宁%傅民魁
李小彤%楊雁琪%杜寧%傅民魁
리소동%양안기%두저%부민괴
骨化三醇%年龄因素%牙周膜细胞%白介素1受体
骨化三醇%年齡因素%牙週膜細胞%白介素1受體
골화삼순%년령인소%아주막세포%백개소1수체
Calcitriol%Age factors%Periodontal ligament cell%Interleukin 1 receptor
目的 比较1α,25-羟基维生素D3(1α,25-dihydroxyvitamin D3,VD3)对幼稚和衰老的牙周膜细胞Ⅰ型白介素1受体(interleukin 1 receptor,type Ⅰ,IL-1R Ⅰ)mRNA表达的影响,以探讨增龄因素影响牙槽骨改建的可能机制.方法 小鼠骨髓细胞和成骨细胞共存培养,检测VD3刺激下破骨细胞的生成；半定量反转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测小鼠成骨细胞和骨髓细胞、第3～5代和第17～20代人牙周膜细胞在VD3刺激下IL-1R Ⅰ mRNA的表达.结果 VD3能刺激小鼠骨髓细胞和成骨细胞共存培养系形成不规则形态的多核巨细胞,抗酒石酸酸性磷酸酶活性染色阳性；受VD3刺激共存培养系中成骨细胞表达IL-1R Ⅰ mRNA.VD3能刺激幼稚人牙周膜细胞表达IL-1R Ⅰ mRNA,其与甘油醛-3-磷酸脱氢酶的表达比为41％,而VD3刺激下衰老的人牙周膜细胞依旧不表达IL-1R Ⅰ.结论 衰老的牙周膜细胞在骨代谢调节因子VD3刺激下仍不表达IL-1R Ⅰ,推测是成年牙周组织改建迟缓的可能机制.
目的 比較1α,25-羥基維生素D3(1α,25-dihydroxyvitamin D3,VD3)對幼稚和衰老的牙週膜細胞Ⅰ型白介素1受體(interleukin 1 receptor,type Ⅰ,IL-1R Ⅰ)mRNA錶達的影響,以探討增齡因素影響牙槽骨改建的可能機製.方法 小鼠骨髓細胞和成骨細胞共存培養,檢測VD3刺激下破骨細胞的生成；半定量反轉錄聚閤酶鏈反應(reverse transcription polymerase chain reaction,RT-PCR)檢測小鼠成骨細胞和骨髓細胞、第3～5代和第17～20代人牙週膜細胞在VD3刺激下IL-1R Ⅰ mRNA的錶達.結果 VD3能刺激小鼠骨髓細胞和成骨細胞共存培養繫形成不規則形態的多覈巨細胞,抗酒石痠痠性燐痠酶活性染色暘性；受VD3刺激共存培養繫中成骨細胞錶達IL-1R Ⅰ mRNA.VD3能刺激幼稚人牙週膜細胞錶達IL-1R Ⅰ mRNA,其與甘油醛-3-燐痠脫氫酶的錶達比為41％,而VD3刺激下衰老的人牙週膜細胞依舊不錶達IL-1R Ⅰ.結論 衰老的牙週膜細胞在骨代謝調節因子VD3刺激下仍不錶達IL-1R Ⅰ,推測是成年牙週組織改建遲緩的可能機製.
목적 비교1α,25-간기유생소D3(1α,25-dihydroxyvitamin D3,VD3)대유치화쇠로적아주막세포Ⅰ형백개소1수체(interleukin 1 receptor,type Ⅰ,IL-1R Ⅰ)mRNA표체적영향,이탐토증령인소영향아조골개건적가능궤제.방법 소서골수세포화성골세포공존배양,검측VD3자격하파골세포적생성；반정량반전록취합매련반응(reverse transcription polymerase chain reaction,RT-PCR)검측소서성골세포화골수세포、제3～5대화제17～20대인아주막세포재VD3자격하IL-1R Ⅰ mRNA적표체.결과 VD3능자격소서골수세포화성골세포공존배양계형성불규칙형태적다핵거세포,항주석산산성린산매활성염색양성；수VD3자격공존배양계중성골세포표체IL-1R Ⅰ mRNA.VD3능자격유치인아주막세포표체IL-1R Ⅰ mRNA,기여감유철-3-린산탈경매적표체비위41％,이VD3자격하쇠로적인아주막세포의구불표체IL-1R Ⅰ.결론 쇠로적아주막세포재골대사조절인자VD3자격하잉불표체IL-1R Ⅰ,추측시성년아주조직개건지완적가능궤제.
Objective To compare the 1 α,25-dihydroxyvitamin D3 (VD3)-stimulated interleukin 1 receptor,type Ⅰ (IL-1R type Ⅰ) expression in young and aged human periodontal ligament cells.Methods Osteoclast formation was examined in coculture of mouse bone marrow cells and calvariae ostecblasts in present of VD3.VD3-stimulated IL-1 R type Ⅰ expression was examined by reverse transcription polymerase chain reaction(RT-PCR) method in mouse bone marrow cells,calvariae ostecblasts,3rd-5th passages and 17th-20th passages human periodontal ligament cells.Results VD3 enhanced osteoclast formation powerfully in cocuhure of mouse bone marrow cells and calvariae ostecblasts.Osteoblasts were the response cells for VD3 stimulati in IL-1R type Ⅰ expression in coculture sytstem.VD3 stimulated IL-1R type Ⅰexpression in young periodontal ligament cells.The ratio of IL-1 R type Ⅰ and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was 41％,but not aged human periodontal ligament cells.Conclusions The non-response of aged periodontal ligament cells for VD3-stimulated IL-1 R type Ⅰ expression could be related to the delay in the onset of alveolar bone resorption and tooth movement.
