CAJ | 학술논문

目的探讨在口腔鳞状细胞癌及癌前病变中DJ-1、张力蛋白同源10q丢失的磷酸酶基因(phophatase and tensin homolog deleted on chromosome 10,PTEN)和黏着斑激酶(focal adhesion kinase,FAK)的作用,以期为口腔癌的早期防治及基因靶向治疗提供依据.方法收集广东省口腔医院黏膜牙周科2005年1月至2010年12月的病理标本,包括口腔鳞状细胞癌44例(口腔鳞状细胞癌组)、单纯增生20例(单纯增生组)、异常增生15例(异常增生组)和健康对照标本10例(健康对照组).采用免疫组化方法检测D J-1、PTEN及FAK 3种蛋白的表达.结果免疫组化结果显示DJ-1在健康对照组、单纯增生组、异常增生组及口腔鳞状细胞癌组中的表达分别为3/10、50％ (10/20)、10/15和91％ (40/44),口腔鳞状细胞癌组织中DJ-1的表达显著增高,与其他组相比差异有统计学意义(P＜0.05)；PTEN在健康对照、单纯增生组的阳性表达均为100％,在异常增生及口腔鳞状细胞癌组中的表达分别为13/15与45％(20/44),而FAK在健康对照、单纯增生、异常增生及口腔鳞状细胞癌组中的表达分别为10/10、95％ (19/20)、14/15和80％(35/44),表明PTEN和FAK在OSCC组织中的表达降低,与其他组相比差异均有统计学意义(P＜0.05)；相关性分析表明D J-1与PTEN之间呈负相关关系(r=-0.75,P＜0.05),PTEN和FAK间呈正相关关系(r=0.28,P＜0.05).结论 D J-1、PTEN、FAK在口腔鳞状细胞癌的发生过程中可能起重要作用.
목적 탐토재구강린상세포암급암전병변중DJ-1、장력단백동원10q주실적린산매기인(phophatase and tensin homolog deleted on chromosome 10,PTEN)화점착반격매(focal adhesion kinase,FAK)적작용,이기위구강암적조기방치급기인파향치료제공의거.방법 수집광동성구강의원점막아주과2005년1월지2010년12월적병리표본,포괄구강린상세포암44례(구강린상세포암조)、단순증생20례(단순증생조)、이상증생15례(이상증생조)화건강대조표본10례(건강대조조).채용면역조화방법검측D J-1、PTEN급FAK 3충단백적표체.결과 면역조화결과현시DJ-1재건강대조조、단순증생조、이상증생조급구강린상세포암조중적표체분별위3/10、50％ (10/20)、10/15화91％ (40/44),구강린상세포암조직중DJ-1적표체현저증고,여기타조상비차이유통계학의의(P＜0.05)；PTEN재건강대조、단순증생조적양성표체균위100％,재이상증생급구강린상세포암조중적표체분별위13/15여45％(20/44),이FAK재건강대조、단순증생、이상증생급구강린상세포암조중적표체분별위10/10、95％ (19/20)、14/15화80％(35/44),표명PTEN화FAK재OSCC조직중적표체강저,여기타조상비차이균유통계학의의(P＜0.05)；상관성분석표명D J-1여PTEN지간정부상관관계(r=-0.75,P＜0.05),PTEN화FAK간정정상관관계(r=0.28,P＜0.05).결론 D J-1、PTEN、FAK재구강린상세포암적발생과정중가능기중요작용.
Objective To investigate the expression of DJ-1,phophatase and tensin homolog deleted on chromosome 10 (PTEN) and focal adhesion(FAK) in patients with oral squamous cell carcinoma (OSCC) and precancerous lesions.Methods Immunohistochemical staining (SP method) was used to detect the expression of DJ-1 and PTEN in the biopsy mucosa tissues from 44 cases of oral squamous cell carcinoma,20 cases of hyperplasia of oral mucosa,15 cases of dysplasia of oral mucosa and 10 cases of normal mucosa.Results The positive rate of DJ-1 in OSCC was 91％ (40/44),which was significandy higher than that in other non-malignant groups including hyperplasia(50％),dysplasia (67％)and normal mucosa(30％) (P ＜ 0.05).The positive rates of PTEN was 45％ (20/44) in OSCC,which were significantly lower than that in other non-malignant groups (P ＜ 0.05) including hyperplasia (100％),dysplasia (87％)and normal mucosa(100％) (P ＜0.05).The positive rates of FAK was 80％ (35/44) in OSCC,which were significantly lower than that in other non-malignant groups (P ＜ 0.05) including hyperplasia(100％),dysplasia(95％) and normal mucosa(100％) (P ＜ 0.05).In all of these tissues,a negative correlation was found between the expression of DJ-1 and PTEN (r =-0.75,P ＜ 0.05),while a positive correlation was found between the expression of DJ-1 and PTEN (r =0.28,P ＜ 0.05).Conclusions DJ-1,PTEN and FAK may play an important role in the pathogenesis of OSCC.