中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
1期
23-26
,共4页
神经生长因子%明胶海绵,吸收性%牙种植体%骨结合
神經生長因子%明膠海綿,吸收性%牙種植體%骨結閤
신경생장인자%명효해면,흡수성%아충식체%골결합
Nerve growth factor%Gelatin sponge,absorbable%Dental Implants%Synostosis
目的 观察外源性神经生长因子(nerve growth factor,NGF)-明胶海绵(gelatin sponge,GS)复合体(NGF-GS)对牙种植体周围骨缺损的修复作用.方法 选择健康成年比格犬6只,分别拔除下颌双侧第一、二前磨牙,于远中拔牙窝行即刻种植,在种植体近中制作5 mm×3 mm×5 mm骨缺损.实验组植入NGF-GS(含NGF 10μg),对照组植入GS,空白组不植入任何物质.分别于术后4、8和12周各处死动物2只并获取标本,制作种植体骨磨片,亚甲基蓝-酸性品红法染色,进行大体、放射学、组织学观察和骨计量学分析.结果 术后4、8周3组种植体周骨缺损的新生骨密度低于周围正常骨组织.术后4周实验组种植体骨结合率[(57.7±6.4)％]显著高于对照组[(44.2±3.3)％]和空白组[(31.2±3.1)％](P＜0.01),且对照组种植体骨结合率显著高于空白组(P＜0.0l)；术后8周实验组种植体骨结合率[(94.8±7.7)％]略高于对照组[(83.0±4.1)％]和空白组[(86.4±6.3)％],差异无统计学意义(P＞0.05)；术后12周各组骨密度与正常骨组织接近,种植体骨结合率无明显差异.结论 NGF-GS在种植体骨结合早期能够增加口腔种植体周围新生骨小梁的面积,加速新生骨组织矿化,缩短骨结合时间,提高种植体骨结合率.
目的 觀察外源性神經生長因子(nerve growth factor,NGF)-明膠海綿(gelatin sponge,GS)複閤體(NGF-GS)對牙種植體週圍骨缺損的脩複作用.方法 選擇健康成年比格犬6隻,分彆拔除下頜雙側第一、二前磨牙,于遠中拔牙窩行即刻種植,在種植體近中製作5 mm×3 mm×5 mm骨缺損.實驗組植入NGF-GS(含NGF 10μg),對照組植入GS,空白組不植入任何物質.分彆于術後4、8和12週各處死動物2隻併穫取標本,製作種植體骨磨片,亞甲基藍-痠性品紅法染色,進行大體、放射學、組織學觀察和骨計量學分析.結果 術後4、8週3組種植體週骨缺損的新生骨密度低于週圍正常骨組織.術後4週實驗組種植體骨結閤率[(57.7±6.4)％]顯著高于對照組[(44.2±3.3)％]和空白組[(31.2±3.1)％](P＜0.01),且對照組種植體骨結閤率顯著高于空白組(P＜0.0l)；術後8週實驗組種植體骨結閤率[(94.8±7.7)％]略高于對照組[(83.0±4.1)％]和空白組[(86.4±6.3)％],差異無統計學意義(P＞0.05)；術後12週各組骨密度與正常骨組織接近,種植體骨結閤率無明顯差異.結論 NGF-GS在種植體骨結閤早期能夠增加口腔種植體週圍新生骨小樑的麵積,加速新生骨組織礦化,縮短骨結閤時間,提高種植體骨結閤率.
목적 관찰외원성신경생장인자(nerve growth factor,NGF)-명효해면(gelatin sponge,GS)복합체(NGF-GS)대아충식체주위골결손적수복작용.방법 선택건강성년비격견6지,분별발제하합쌍측제일、이전마아,우원중발아와행즉각충식,재충식체근중제작5 mm×3 mm×5 mm골결손.실험조식입NGF-GS(함NGF 10μg),대조조식입GS,공백조불식입임하물질.분별우술후4、8화12주각처사동물2지병획취표본,제작충식체골마편,아갑기람-산성품홍법염색,진행대체、방사학、조직학관찰화골계량학분석.결과 술후4、8주3조충식체주골결손적신생골밀도저우주위정상골조직.술후4주실험조충식체골결합솔[(57.7±6.4)％]현저고우대조조[(44.2±3.3)％]화공백조[(31.2±3.1)％](P＜0.01),차대조조충식체골결합솔현저고우공백조(P＜0.0l)；술후8주실험조충식체골결합솔[(94.8±7.7)％]략고우대조조[(83.0±4.1)％]화공백조[(86.4±6.3)％],차이무통계학의의(P＞0.05)；술후12주각조골밀도여정상골조직접근,충식체골결합솔무명현차이.결론 NGF-GS재충식체골결합조기능구증가구강충식체주위신생골소량적면적,가속신생골조직광화,축단골결합시간,제고충식체골결합솔.
Objective To observe the peri-implant osseointegration after the application of exogenous nerve growth factor (NGF)-gelatin sponge(GS) composite.Methods Six beagle dogs were used to establish bi-mandible simultaneous implant model after the first and second premolar extraction.Then the dental implants were implanted into the distal socket while the bone defect was made in the mesial socket of each mandible.Then the NGF-GS was implanted into the defects as NGF-GS group,the gelatin sponge alone was implanted as GS control and the control group was left empty.Two dogs were sacrificed each time at 4 weeks,8 weeks and 12 weeks postoperatively.Specimens were subjected to general observation,radiography,bone histological and histomorphometric analysis for the new bone formation.The data were analyzed with SPSS 11.5 software package.Results The bone density in the defects around implants at 4 and 8 weeks postoperatively was lower than the normal bone.The bone-implant contact ratio in the NGF-GS group[(57.7 ± 6.4)％] was significantly higher than that in the GS control group and the control group [the ratio were(44.2 ± 3.3)％ and(31.2 ± 3.1)％] (P ＜ 0.01) at 4 weeks postoperatively,the bone-implant contact ratio in the GS control group was also significantly higher than that in the control group (P＜0.01) at that time.The bone-implant contact ratio in the NGF-GS group[(94.8 ± 7.7)％] was slightly higher than that in the GS control group and the control group [the ratio were (83.0 ± 4.1) ％ and (86.4 ± 6.3) ％] at 8 weeks postoperatively,but there was no statistical difference (P ＞ 0.05).The bone density in the defects around implants at 12 weeks was almost the same as the normal bone,there was no difference of the bone-implant contact ratio.Conclusions NGF-GS application could increase new bone formation,accelerate maturation of trabecular bone around the implants and shorten the period of osseointegration.
