CAJ | 학술논문

目的评价10％酪蛋白磷酸多肽钙磷复合体(complex of casein phosphopeptide and amorphous calcium phosphate,CPP-ACP)对抑制离体牙牙釉质脱矿及促进再矿化的功效,以期为临床防龋提供实验依据.方法将因正畸需要拔除的离体前磨牙18颗从冠根向一分为二,制成36个实验样本,用自凝材料包埋于塑料圈内,根据扫描电镜样品的要求打磨抛光后牙釉质面积为3 mm×3 mm.用随机数字表法将实验样本随机分为6组,每组6个,分别为不酸蚀组、酸蚀组及实验A、B、C、D组,不酸蚀组不进行酸蚀及CPP-ACP浸泡,酸蚀组仅用37.5％磷酸酸蚀浸泡牙釉质2 min,实验组用37.5％磷酸酸蚀浸泡牙釉质2 min后分别用CPP-ACP涂搽牙面3 min,置于蒸馏水中浸泡24h,待第2天在同一时间涂搽CPP-ACP 3 min并进行蒸馏水浸泡24 h,实验A、B、C、D组分别应用CPP-ACP浸泡7、14、21、28 d.扫描电镜观察并用能谱检测钙的质量分数,统计分析各组釉柱再矿化与浸泡时间的关系.结果扫描电镜观察结果显示,CPP-ACP浸泡后实验A、B、C、D组釉质表面有大量的矿物质沉积,填补了釉质表面局限性的小凹陷；实验D组的釉质表面矿物质沉积较实验A、B、C组致密.实验A、B、C、D组釉质表面钙质量分数[分别为(66.53 ±0.63)、(67.00 ±0.49)、(67.07±0.24)、(67.18 ±0.50)]与酸蚀组(65.74 ±0.68)相比差异均有统计学意义(P＜0.05)；实验A、B、C、D组间两两比较差异均无统计学意义(P＞0.05).结论 CPP-ACP在体外实验7d能使离体牙牙釉质在蒸馏水环境中抑制脱矿和促进再矿化,28 d可以加强牙釉质再矿化的功效.
목적 평개10％락단백린산다태개린복합체(complex of casein phosphopeptide and amorphous calcium phosphate,CPP-ACP)대억제리체아아유질탈광급촉진재광화적공효,이기위림상방우제공실험의거.방법 장인정기수요발제적리체전마아18과종관근향일분위이,제성36개실험양본,용자응재료포매우소료권내,근거소묘전경양품적요구타마포광후아유질면적위3 mm×3 mm.용수궤수자표법장실험양본수궤분위6조,매조6개,분별위불산식조、산식조급실험A、B、C、D조,불산식조불진행산식급CPP-ACP침포,산식조부용37.5％린산산식침포아유질2 min,실험조용37.5％린산산식침포아유질2 min후분별용CPP-ACP도차아면3 min,치우증류수중침포24h,대제2천재동일시간도차CPP-ACP 3 min병진행증류수침포24 h,실험A、B、C、D조분별응용CPP-ACP침포7、14、21、28 d.소묘전경관찰병용능보검측개적질량분수,통계분석각조유주재광화여침포시간적관계.결과 소묘전경관찰결과현시,CPP-ACP침포후실험A、B、C、D조유질표면유대량적광물질침적,전보료유질표면국한성적소요함；실험D조적유질표면광물질침적교실험A、B、C조치밀.실험A、B、C、D조유질표면개질량분수[분별위(66.53 ±0.63)、(67.00 ±0.49)、(67.07±0.24)、(67.18 ±0.50)]여산식조(65.74 ±0.68)상비차이균유통계학의의(P＜0.05)；실험A、B、C、D조간량량비교차이균무통계학의의(P＞0.05).결론 CPP-ACP재체외실험7d능사리체아아유질재증류수배경중억제탈광화촉진재광화,28 d가이가강아유질재광화적공효.
Objective To investigate the effect of the complex of casein phosphopeptide and amorphous calcium phosphate(CPP-ACP) on the demineralization and remineralization of dental enamel in vitro.Methods Premolars extracted from patients receiving orthodontic treatment were cut into two slabs,which were embedded and polished.The slabs were randomly divided into non-acid etching group,acid etching group(immersed in 37.5％ phosphoric acid for 2 minutes),experimental group A (immersed in 37.5％ phosphoric acid for 2 minutes and wiped by CPP-ACP for 3 minutes,then immersed in distilled water for 7 days),experimental group B(immersed in 37.5％ Phosphoric acid for 2 minutes and wiped by CPP-ACP for 3 minutes,then immersed in distilled water for 14 days),experimental group C (immersed in 37.5％ phosphoric acid for 2 minutes and wiped by CPP-ACP for 3 minutes,then immersed in distilled water for 21 days),experimental group D(immersed in 37.5％ phosphoric acid for 2 minutes and wiped by CPP-ACP for 3 minutes,then immersed in distilled water for 28 days),with 6 slabs in each group.The mineral content was determined by scanning electron microscope.Results There was a large amount of mineral deposited on enamel surface in experimental group A,B,C,D.The calcium content of experimental group D was higher than those of other 3 experimental groups.The calcium content of experimental group A,B,C,D(66.53 ± 0.63,67.00 ± 0.49,67.07 ± 0.24,67.18 ± 0.50) was higher than that of acid etching group(65.74 ± 0.68) (P ＜ 0.05).There was no significant difference of the calcium content among experimental group A,B,C,D (P ＞ 0.05).Conclusions CPP-ACP can reduce enamel demineralization and promote remineralization in vitro.