中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
1期
53-57
,共5页
X线显微体层摄影术%下颌骨%功能减退
X線顯微體層攝影術%下頜骨%功能減退
X선현미체층섭영술%하합골%공능감퇴
X-ray microtomography%Mandible%Hypofunction
目的 探讨实验性咬合功能减退对大鼠下颌骨三维显微结构的影响,以期为研究应力改变对颌骨形态、结构等的影响提供实验依据.方法 40只雄性SD大鼠采用随机数字表法随机分为咬合功能减退组和对照组,每组20只.在咬合功能减退组大鼠上下颌前牙戴金属顶盖,使后牙无咬合接触,建立咬合功能减退模型；对照组大鼠前牙戴金属环,后牙保持正常咬合接触.于实验开始时(0周)和实验2、4、6、8周末分别麻醉并处死每组4只大鼠,测量体质量,并用显微CT测量下颌牙槽骨骨松质的三维显微结构.结果 与对照组相比,实验4周咬合功能减退组大鼠下颌牙槽骨骨体积/总体积开始降低[对照组(0.65±0.05)％,咬合功能减退组(0.60±0.05)％,P＜0.05],并持续到实验8周[对照组(0.72士0.06)％,咬合功能减退组(0.51±0.07)％,P＜0.01]；骨小梁厚度也出现类似变化,表现为实验4、6、8周咬合功能减退组显著低于对照组[对照组分别为(168±15)、(170±25)和(180±18) μm,咬合功能减退组分别为(152±20)、(145±19)和(142±15) μm,P＜0.05]；咬合功能减退组大鼠下颌牙槽骨骨小梁间隙逐渐升高,实验4、6、8周时与对照组差异有统计学意义[对照组分别为(264 ±21)、(284±17)和(282±26) μm,咬合功能减退组分别为(306±30)、(316±18)和(332±18) μm,P＜0.05]；而骨小梁数量在实验6周开始下降[对照组(3.59±0.22) mm-1,咬合功能减退组(3.03±0.31) mm-1,P＜0.05],一直持续到实验8周[对照组(3.66±0.24) mm-1,咬合功能减退组(2.85 ±0.18) mm-1,P＜0.01].结论 咬合功能减退可导致下颌骨骨松质三维显微结构改变,出现骨丢失.
目的 探討實驗性咬閤功能減退對大鼠下頜骨三維顯微結構的影響,以期為研究應力改變對頜骨形態、結構等的影響提供實驗依據.方法 40隻雄性SD大鼠採用隨機數字錶法隨機分為咬閤功能減退組和對照組,每組20隻.在咬閤功能減退組大鼠上下頜前牙戴金屬頂蓋,使後牙無咬閤接觸,建立咬閤功能減退模型；對照組大鼠前牙戴金屬環,後牙保持正常咬閤接觸.于實驗開始時(0週)和實驗2、4、6、8週末分彆痳醉併處死每組4隻大鼠,測量體質量,併用顯微CT測量下頜牙槽骨骨鬆質的三維顯微結構.結果 與對照組相比,實驗4週咬閤功能減退組大鼠下頜牙槽骨骨體積/總體積開始降低[對照組(0.65±0.05)％,咬閤功能減退組(0.60±0.05)％,P＜0.05],併持續到實驗8週[對照組(0.72士0.06)％,咬閤功能減退組(0.51±0.07)％,P＜0.01]；骨小樑厚度也齣現類似變化,錶現為實驗4、6、8週咬閤功能減退組顯著低于對照組[對照組分彆為(168±15)、(170±25)和(180±18) μm,咬閤功能減退組分彆為(152±20)、(145±19)和(142±15) μm,P＜0.05]；咬閤功能減退組大鼠下頜牙槽骨骨小樑間隙逐漸升高,實驗4、6、8週時與對照組差異有統計學意義[對照組分彆為(264 ±21)、(284±17)和(282±26) μm,咬閤功能減退組分彆為(306±30)、(316±18)和(332±18) μm,P＜0.05]；而骨小樑數量在實驗6週開始下降[對照組(3.59±0.22) mm-1,咬閤功能減退組(3.03±0.31) mm-1,P＜0.05],一直持續到實驗8週[對照組(3.66±0.24) mm-1,咬閤功能減退組(2.85 ±0.18) mm-1,P＜0.01].結論 咬閤功能減退可導緻下頜骨骨鬆質三維顯微結構改變,齣現骨丟失.
목적 탐토실험성교합공능감퇴대대서하합골삼유현미결구적영향,이기위연구응력개변대합골형태、결구등적영향제공실험의거.방법 40지웅성SD대서채용수궤수자표법수궤분위교합공능감퇴조화대조조,매조20지.재교합공능감퇴조대서상하합전아대금속정개,사후아무교합접촉,건립교합공능감퇴모형；대조조대서전아대금속배,후아보지정상교합접촉.우실험개시시(0주)화실험2、4、6、8주말분별마취병처사매조4지대서,측량체질량,병용현미CT측량하합아조골골송질적삼유현미결구.결과 여대조조상비,실험4주교합공능감퇴조대서하합아조골골체적/총체적개시강저[대조조(0.65±0.05)％,교합공능감퇴조(0.60±0.05)％,P＜0.05],병지속도실험8주[대조조(0.72사0.06)％,교합공능감퇴조(0.51±0.07)％,P＜0.01]；골소량후도야출현유사변화,표현위실험4、6、8주교합공능감퇴조현저저우대조조[대조조분별위(168±15)、(170±25)화(180±18) μm,교합공능감퇴조분별위(152±20)、(145±19)화(142±15) μm,P＜0.05]；교합공능감퇴조대서하합아조골골소량간극축점승고,실험4、6、8주시여대조조차이유통계학의의[대조조분별위(264 ±21)、(284±17)화(282±26) μm,교합공능감퇴조분별위(306±30)、(316±18)화(332±18) μm,P＜0.05]；이골소량수량재실험6주개시하강[대조조(3.59±0.22) mm-1,교합공능감퇴조(3.03±0.31) mm-1,P＜0.05],일직지속도실험8주[대조조(3.66±0.24) mm-1,교합공능감퇴조(2.85 ±0.18) mm-1,P＜0.01].결론 교합공능감퇴가도치하합골골송질삼유현미결구개변,출현골주실.
Objective To investigate the effects of experimental occlusal hypofunction on the architecture of rat' s mandibular bone.Methods Forty Sprague-Dawley (SD) male rats were randomly divided into experimental occlusal hypofunction group and control group,with 20 rats in each group.Experimental occlusal hypofunction group rats were fitted with a metal cap to the maxillary and mandibular incisor,to eliminate the occlusal forces on rat molars.In control group,a metal ring was fitted to the maxillary and mandibular incisor to maintain molar occlusal contact.Then the rats were killed under deep anaesthesia at 0 week and at the end of 2,4,6,and 8 weeks,and the mandibles were immediately removed for measurement of the three-dimensional architecture of cancellous bone in the alveolar process with micro-CT.Results At the end of week 4,the bone volume/total volume (BV/TV) in the hypofunction group decreased compared with the control group [control group:(0.65 ± 0.05) ％,experimental group:(0.60 ± 0.05) ％,P ＜ 0.05] and lasted until the end of the experiment [control group:(0.72 ± 0.06) ％,experimental group:(0.51 ± 0.07)％,P ＜ 0.01].The similar result was also observed in trabecula thickness (Tb.Th) in experimental animals[control group:(168 ± 15),(170 ± 25),(180 ± 18) μm,experimental group:(152 ± 20),(145 ± 19),(142 ± 15) μm,P ＜ 0.05].The trabecula spacing (Tb.Sp)in the hypofunction group increased from the end of week 4 to the end of week 8 compared with the matched control groups [control group:(264 ± 21),(284 ± 17),(282 ± 26) μm,experimental group:(306±30),(316 ± 18),(332 ± 18) μm,P ＜0.05].The trabecula number (Tb.N) of experimental animals decreased from the end of week 6 [control group:(3.59 ± 0.22) mm-1 experimental group:(3.03 ± 0.31) mm-1 P ＜ 0.05] to the end of week 8 [control group:(3.66 ± 0.24) mm-1,experimental group:(2.85 ± 0.18) mm-1,P＜0.01].Conclusions Experimental occlusal hypofunction can result in the change of mandibular alveolar process three-dimensional architecture.
