中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
3期
150-154
,共5页
肖玥%孔祥波%陈绛媛%阮毅
肖玥%孔祥波%陳絳媛%阮毅
초모%공상파%진강원%원의
牙周炎%流产,习惯性%白细胞介素4%干扰素γ
牙週炎%流產,習慣性%白細胞介素4%榦擾素γ
아주염%유산,습관성%백세포개소4%간우소γ
Periodontitis%Abortion,habitual%Interleukin-4%Interferon-γ
目的 检测复发性自然流产(recurrent spontaneous abortion,RSA)孕妇外周血及其龈沟液中白介素4(interleukin-4,IL-4)、干扰素γ(interferon-γ,IFN-γ)的含量并进行比较,探讨牙周炎是否会引起体液免疫失调,引起孕妇宫腔内免疫环境向T辅助细胞1型(T-helper 1,Th1)漂移并引发流产.方法 检查RSA孕妇50例(孕5至28周)(RSA组)和足月待产孕妇40例(孕39至41周)(对照组)的牙周状况,收集其龈沟液和静脉血,对其进行口腔卫生宣教,酶联免疫吸附测定法检测龈沟液和静脉血中IFN-γ、IL-4的水平.结果 RSA组9例患牙周炎,患病率为18％ (9/50),对照组5例患牙周炎,患病率为13％ (5/40),两组牙周炎的患病率差异有统计学意义(P ＜0.05)；RSA组的探诊深度、附着丧失及出血指数均显著高于对照组(P＜0.05).RSA组孕妇龈沟液及静脉血中IFN-γ含量[分别为(52.98±17.56)、(27.79±3.59) ng/L]均显著高于对照组[分别为(25.25±7.93)、(18.39±2.65) ng/L],P＜0.05；RSA组孕妇龈沟液及静脉血中IL-4的含量[分别为(15.43±1.77)、(15.88 ±0.95) ng/L]均显著低于对照组[(19.62±4.04)、(22.98±4.30) ng/L],P＜0.01.相同组内静脉血与龈沟液中IFN-γ、IL-4的含量均呈正相关关系(r值均＞0.8,P＜0.001).结论 RSA孕妇患牙周炎的几率较高,牙周组织中IFN-γ、IL-4浓度的改变会进一步引发静脉血中IFN-γ、IL-4的浓度改变,使Th1-Th2平衡向Th1漂移；RSA孕妇与其患牙周炎存在相关性.
目的 檢測複髮性自然流產(recurrent spontaneous abortion,RSA)孕婦外週血及其齦溝液中白介素4(interleukin-4,IL-4)、榦擾素γ(interferon-γ,IFN-γ)的含量併進行比較,探討牙週炎是否會引起體液免疫失調,引起孕婦宮腔內免疫環境嚮T輔助細胞1型(T-helper 1,Th1)漂移併引髮流產.方法 檢查RSA孕婦50例(孕5至28週)(RSA組)和足月待產孕婦40例(孕39至41週)(對照組)的牙週狀況,收集其齦溝液和靜脈血,對其進行口腔衛生宣教,酶聯免疫吸附測定法檢測齦溝液和靜脈血中IFN-γ、IL-4的水平.結果 RSA組9例患牙週炎,患病率為18％ (9/50),對照組5例患牙週炎,患病率為13％ (5/40),兩組牙週炎的患病率差異有統計學意義(P ＜0.05)；RSA組的探診深度、附著喪失及齣血指數均顯著高于對照組(P＜0.05).RSA組孕婦齦溝液及靜脈血中IFN-γ含量[分彆為(52.98±17.56)、(27.79±3.59) ng/L]均顯著高于對照組[分彆為(25.25±7.93)、(18.39±2.65) ng/L],P＜0.05；RSA組孕婦齦溝液及靜脈血中IL-4的含量[分彆為(15.43±1.77)、(15.88 ±0.95) ng/L]均顯著低于對照組[(19.62±4.04)、(22.98±4.30) ng/L],P＜0.01.相同組內靜脈血與齦溝液中IFN-γ、IL-4的含量均呈正相關關繫(r值均＞0.8,P＜0.001).結論 RSA孕婦患牙週炎的幾率較高,牙週組織中IFN-γ、IL-4濃度的改變會進一步引髮靜脈血中IFN-γ、IL-4的濃度改變,使Th1-Th2平衡嚮Th1漂移；RSA孕婦與其患牙週炎存在相關性.
목적 검측복발성자연유산(recurrent spontaneous abortion,RSA)잉부외주혈급기간구액중백개소4(interleukin-4,IL-4)、간우소γ(interferon-γ,IFN-γ)적함량병진행비교,탐토아주염시부회인기체액면역실조,인기잉부궁강내면역배경향T보조세포1형(T-helper 1,Th1)표이병인발유산.방법 검사RSA잉부50례(잉5지28주)(RSA조)화족월대산잉부40례(잉39지41주)(대조조)적아주상황,수집기간구액화정맥혈,대기진행구강위생선교,매련면역흡부측정법검측간구액화정맥혈중IFN-γ、IL-4적수평.결과 RSA조9례환아주염,환병솔위18％ (9/50),대조조5례환아주염,환병솔위13％ (5/40),량조아주염적환병솔차이유통계학의의(P ＜0.05)；RSA조적탐진심도、부착상실급출혈지수균현저고우대조조(P＜0.05).RSA조잉부간구액급정맥혈중IFN-γ함량[분별위(52.98±17.56)、(27.79±3.59) ng/L]균현저고우대조조[분별위(25.25±7.93)、(18.39±2.65) ng/L],P＜0.05；RSA조잉부간구액급정맥혈중IL-4적함량[분별위(15.43±1.77)、(15.88 ±0.95) ng/L]균현저저우대조조[(19.62±4.04)、(22.98±4.30) ng/L],P＜0.01.상동조내정맥혈여간구액중IFN-γ、IL-4적함량균정정상관관계(r치균＞0.8,P＜0.001).결론 RSA잉부환아주염적궤솔교고,아주조직중IFN-γ、IL-4농도적개변회진일보인발정맥혈중IFN-γ、IL-4적농도개변,사Th1-Th2평형향Th1표이；RSA잉부여기환아주염존재상관성.
Objective To investigate the relationship between recurrent spontaneous abortion (RSA) and the level of interferon-γ(IFN-γ) and interleukin-4 (IL-4) in peripheral blood and gingival crevicular fluid of patients with chronic periodontitis.Methods Fifty pregnant women with recurrent spontaneous abortion(gestational age: 5-28 weeks) and 40pregnant women (gestational age: 39-41 weeks)were included and their periodontal status was examined.The level of IFN-γ and IL-4 in gingival crevicular fluid and peripheral blood was detected by enzyme-linked immunosorbent assay.Results Nine RSA women and 5 in control group had periodontitis.The prevalence of periodontitis in RSA group was 18％ (9/50) and 13％ (5/40) in the control group(P ＜0.05).The probing depth,attachment loss and bleeding index in RSA group were higher than those in the control group(P ＜0.05).The level of IFN-γwas (52.98 ± 17.56) ng/L in gingival crevicular fluid of RSA group and (25.25 ± 7.93) ng/L in control group(P ＜ 0.01).The level of IL-4 was (15.43 ± 1.77) ng/L in gingival crevicular fluid of RSA group and (19.62 ± 4.04) ng/L in control group (P ＜0.01).The content of IFN-γwas (27.79 ± 3.59) ng/L in peripheral blood of RSA group and (18.39 ±2.65) ng/L in control group(P ＜0.05).The content of IL-4 was (15.88 ± 0.95) ng/L in peripheral blood of RSA group and (22.98 ± 4.30) ng/L in control group (P ＜ 0.001).The content of cytokines in gingival crevicular fluid and peripheral blood in the same group was positively related (r ＞ 0.8,P ＜ 0.001).Conclusions Pregnant women of RSA have a higher chance of getting periodontitis.The change in the level of cytokines in periodontal tissue could result in excursion of maternal intrauterine immune environment to T-helper 1 (Th1).Periodontitis was related to RSA.
