中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
4期
234-238
,共5页
血管内皮生长因子类%纳米复合物%生物活性玻璃%人牙髓细胞
血管內皮生長因子類%納米複閤物%生物活性玻璃%人牙髓細胞
혈관내피생장인자류%납미복합물%생물활성파리%인아수세포
Vascular endothelial growth factors%Nanocomposites%Bioactive glasses%Human dental pulp cells
目的 体外观察、比较新型纳米生物活性玻璃nano-58S和传统生物活性玻璃45S5对人牙髓细胞增殖及血管内皮生长因子(vascular endothelial growth factor,VEGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的作用,以期为进一步研究生物活性玻璃是否可在牙髓再生的组织工程学中发挥作用奠定基础.方法 实验组用45S5 (45S5组)、nano-58S(nano-58S组)生物活性玻璃浸提液培养人牙髓细胞,空白对照组用达尔伯克改良伊格尔培养基(Dulbecco's modified Eagle's medium,DMEM)培养,每组3孔.培养人牙髓细胞1、2、3d分别采用甲基噻唑基四唑法观察浸提液对细胞增殖的影响,用实时荧光定量反转录PCR和酶联免疫吸附测定法检测VEGF、bFGF基因表达水平和蛋白分泌量.结果 人牙髓细胞培养第2天,45S5组、nano-58S组细胞增殖率分别为空白对照组的(134.5±5.0)％和(146.3±19.8)％,与空白对照组相比差异均有统计学意义(P＜0.05)；45S5组和nano-58S组在第1天VEGF蛋白分泌量分别为(189.29±4.64)和(216.18±14.67) ng/L,均显著高于空白对照组[(159.03±11.69) ng/L] (P＜0.05),nano-58S组VEGF蛋白分泌量显著高于45S5组(P＜0.05)；两个实验组的bFGF蛋白分泌量亦显著高于空白对照组(P＜0.05)；45S5组和nano-58S组的VEGF基因表达量在第1天分别为(1.70±0.19)和(1.63±0.42),均显著高于空白对照组(1.00 +0.02) (P ＜0.05)；bFGF基因相对表达量在第3天分别为(1.49±0.02)和(2.30±0.04),亦均显著高于空白对照组(1.29 ±0.04) (P＜0.05).结论 生物活性玻璃可促进人牙髓细胞的增殖及VEGF、bFGF的基因表达和蛋白分泌,nano-58S比45S5的促进作用更显著.
目的 體外觀察、比較新型納米生物活性玻璃nano-58S和傳統生物活性玻璃45S5對人牙髓細胞增殖及血管內皮生長因子(vascular endothelial growth factor,VEGF)、堿性成纖維細胞生長因子(basic fibroblast growth factor,bFGF)的作用,以期為進一步研究生物活性玻璃是否可在牙髓再生的組織工程學中髮揮作用奠定基礎.方法 實驗組用45S5 (45S5組)、nano-58S(nano-58S組)生物活性玻璃浸提液培養人牙髓細胞,空白對照組用達爾伯剋改良伊格爾培養基(Dulbecco's modified Eagle's medium,DMEM)培養,每組3孔.培養人牙髓細胞1、2、3d分彆採用甲基噻唑基四唑法觀察浸提液對細胞增殖的影響,用實時熒光定量反轉錄PCR和酶聯免疫吸附測定法檢測VEGF、bFGF基因錶達水平和蛋白分泌量.結果 人牙髓細胞培養第2天,45S5組、nano-58S組細胞增殖率分彆為空白對照組的(134.5±5.0)％和(146.3±19.8)％,與空白對照組相比差異均有統計學意義(P＜0.05)；45S5組和nano-58S組在第1天VEGF蛋白分泌量分彆為(189.29±4.64)和(216.18±14.67) ng/L,均顯著高于空白對照組[(159.03±11.69) ng/L] (P＜0.05),nano-58S組VEGF蛋白分泌量顯著高于45S5組(P＜0.05)；兩箇實驗組的bFGF蛋白分泌量亦顯著高于空白對照組(P＜0.05)；45S5組和nano-58S組的VEGF基因錶達量在第1天分彆為(1.70±0.19)和(1.63±0.42),均顯著高于空白對照組(1.00 +0.02) (P ＜0.05)；bFGF基因相對錶達量在第3天分彆為(1.49±0.02)和(2.30±0.04),亦均顯著高于空白對照組(1.29 ±0.04) (P＜0.05).結論 生物活性玻璃可促進人牙髓細胞的增殖及VEGF、bFGF的基因錶達和蛋白分泌,nano-58S比45S5的促進作用更顯著.
목적 체외관찰、비교신형납미생물활성파리nano-58S화전통생물활성파리45S5대인아수세포증식급혈관내피생장인자(vascular endothelial growth factor,VEGF)、감성성섬유세포생장인자(basic fibroblast growth factor,bFGF)적작용,이기위진일보연구생물활성파리시부가재아수재생적조직공정학중발휘작용전정기출.방법 실험조용45S5 (45S5조)、nano-58S(nano-58S조)생물활성파리침제액배양인아수세포,공백대조조용체이백극개량이격이배양기(Dulbecco's modified Eagle's medium,DMEM)배양,매조3공.배양인아수세포1、2、3d분별채용갑기새서기사서법관찰침제액대세포증식적영향,용실시형광정량반전록PCR화매련면역흡부측정법검측VEGF、bFGF기인표체수평화단백분비량.결과 인아수세포배양제2천,45S5조、nano-58S조세포증식솔분별위공백대조조적(134.5±5.0)％화(146.3±19.8)％,여공백대조조상비차이균유통계학의의(P＜0.05)；45S5조화nano-58S조재제1천VEGF단백분비량분별위(189.29±4.64)화(216.18±14.67) ng/L,균현저고우공백대조조[(159.03±11.69) ng/L] (P＜0.05),nano-58S조VEGF단백분비량현저고우45S5조(P＜0.05)；량개실험조적bFGF단백분비량역현저고우공백대조조(P＜0.05)；45S5조화nano-58S조적VEGF기인표체량재제1천분별위(1.70±0.19)화(1.63±0.42),균현저고우공백대조조(1.00 +0.02) (P ＜0.05)；bFGF기인상대표체량재제3천분별위(1.49±0.02)화(2.30±0.04),역균현저고우공백대조조(1.29 ±0.04) (P＜0.05).결론 생물활성파리가촉진인아수세포적증식급VEGF、bFGF적기인표체화단백분비,nano-58S비45S5적촉진작용경현저.
Objective To investigate the effects of bioactive glasses (BG) including 45S5 and nano-58S on proliferation,angiogenic markers vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) secretion and gene expression of human dental pulp cells(HDPC).Methods HDPC of 4th passage were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained 0.1 g/L 45 S5 or nano-58S ionic dissolution products.Meanwhile HDPC were cultured in DMEM without BG as control group.Proliferation of the cells was evaluated with methyl thiazolyl tetrazolium(MTT) assay on day 1,2,3.Quantitative real-time PCR and quantitative sandwich enzyme immunoassays were used to test VEGF and bFGF gene expression and protein secretion of HDPC on day 1,2,3.Results The relative growth rate (RGR) of 45S5 and nano-58S groups were(134.5 ± 5.0)％ and (146.3 ± 19.8)％,which was significantly different from that of control group (P ＜ 0.05).The quantity of VEGF secretion of two experimental groups were(189.29 ± 4.64) and (216.18 ± 14.67) ng/L,respectively,significantly higher than that of the control group [(159.03 ± 11.69) ng/L] (P ＜ 0.05).Furthermore,the nano-58S group secreted much more VEGF than 45S5 group(P ＜ 0.05).bFGF secretion of HDPC was also enhanced by both 45S5 and nano-58S bioactive glasses.The VEGF gene expression of 45S5 and nano-58S on day 1 were (1.70 ±0.19) and (1.63 ±0.42),while the bFGF gene expressin on day 3 were (1.49 ± 0.02) and (2.30 ±0.04),all significantly higher than that of control group (P ＜ 0.05).Conclusions Bioactive glasses can enhance the proliferation,VEGF and bFGF secretion and gene expression of human dental pulp cells.Compared with 45S5,nano-58S showed a higher activation.
