中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
4期
239-243
,共5页
刘宁%李芳%张凌%陈宇江%陈吉华
劉寧%李芳%張凌%陳宇江%陳吉華
류저%리방%장릉%진우강%진길화
基质金属蛋白酶8%拉伸强度%胶原%季铵类化合物
基質金屬蛋白酶8%拉伸彊度%膠原%季銨類化閤物
기질금속단백매8%랍신강도%효원%계안류화합물
Matrix metalloproteinase 8%Tensile strength%Collagen%Quaternary ammonium compound
目的 研究新型双甲基丙烯酰季铵盐单体对基质金属蛋白酶活性的影响,探索提高牙本质粘接耐久性的新方法.方法 使用荧光试剂盒检测质量分数分别为1％、3％、5％和7％的双甲基丙烯酰季铵盐单体[2-甲基丙烯酰氧乙基-正十二烷基-甲基溴化铵(2-methacryloxylethyl dodecylmethyl ammonium bromide,MAE-DB)]对游离型基质金属蛋白酶8(matrix metalloproteinase-8,MMP-8)活性的影响,用酶标仪每隔20 min检测荧光值至3h,以试剂盒提供的抑制剂1,10-二氮菲作为抑制剂对照组.将24颗离体牙制备的牙本质试件完全脱矿后获得胶原纤维试件,通过随机数字表法分成3组(每组50个),分别浸泡于人工唾液、含7％ MAE-DB单体的人工唾液以及含2％氯己定的人工唾液中,经冷热循环5000和10 000次后,检测胶原纤维试件拉伸强度的变化,评价MAE-DB单体对结合型基质金属蛋白酶的作用.透射电镜观察冷热循环后各组胶原纤维试件的形态.结果 1 h 3％ MAE-DB单体对MMP-8活性的抑制百分比为99.53％,显著高于抑制剂对照组(95.71％)(P＜0.05).冷热循环5000次和10 000次后,含7％ MAE-DB组胶原纤维试件的拉伸强度[分别为(10.66±2.12)和(8.89±1.89) MPa]分别显著高于人工唾液组[分别为(7.43±2.27)和(5.46±1.76) MPa]和含2％氯己定人工唾液组[分别为(9.34±2.53)和(7.67 ±2.20) MPa] (P＜0.05).透射电镜显示7％ MAE-DB组胶原纤维微观结构完整,而人工唾液组胶原纤维微观结构紊乱.结论 双甲基丙烯酰季铵盐单体MAE-DB可有效抑制MMP-8的活性,保护胶原纤维,降低胶原纤维在老化过程中的酶解.
目的 研究新型雙甲基丙烯酰季銨鹽單體對基質金屬蛋白酶活性的影響,探索提高牙本質粘接耐久性的新方法.方法 使用熒光試劑盒檢測質量分數分彆為1％、3％、5％和7％的雙甲基丙烯酰季銨鹽單體[2-甲基丙烯酰氧乙基-正十二烷基-甲基溴化銨(2-methacryloxylethyl dodecylmethyl ammonium bromide,MAE-DB)]對遊離型基質金屬蛋白酶8(matrix metalloproteinase-8,MMP-8)活性的影響,用酶標儀每隔20 min檢測熒光值至3h,以試劑盒提供的抑製劑1,10-二氮菲作為抑製劑對照組.將24顆離體牙製備的牙本質試件完全脫礦後穫得膠原纖維試件,通過隨機數字錶法分成3組(每組50箇),分彆浸泡于人工唾液、含7％ MAE-DB單體的人工唾液以及含2％氯己定的人工唾液中,經冷熱循環5000和10 000次後,檢測膠原纖維試件拉伸彊度的變化,評價MAE-DB單體對結閤型基質金屬蛋白酶的作用.透射電鏡觀察冷熱循環後各組膠原纖維試件的形態.結果 1 h 3％ MAE-DB單體對MMP-8活性的抑製百分比為99.53％,顯著高于抑製劑對照組(95.71％)(P＜0.05).冷熱循環5000次和10 000次後,含7％ MAE-DB組膠原纖維試件的拉伸彊度[分彆為(10.66±2.12)和(8.89±1.89) MPa]分彆顯著高于人工唾液組[分彆為(7.43±2.27)和(5.46±1.76) MPa]和含2％氯己定人工唾液組[分彆為(9.34±2.53)和(7.67 ±2.20) MPa] (P＜0.05).透射電鏡顯示7％ MAE-DB組膠原纖維微觀結構完整,而人工唾液組膠原纖維微觀結構紊亂.結論 雙甲基丙烯酰季銨鹽單體MAE-DB可有效抑製MMP-8的活性,保護膠原纖維,降低膠原纖維在老化過程中的酶解.
목적 연구신형쌍갑기병희선계안염단체대기질금속단백매활성적영향,탐색제고아본질점접내구성적신방법.방법 사용형광시제합검측질량분수분별위1％、3％、5％화7％적쌍갑기병희선계안염단체[2-갑기병희선양을기-정십이완기-갑기추화안(2-methacryloxylethyl dodecylmethyl ammonium bromide,MAE-DB)]대유리형기질금속단백매8(matrix metalloproteinase-8,MMP-8)활성적영향,용매표의매격20 min검측형광치지3h,이시제합제공적억제제1,10-이담비작위억제제대조조.장24과리체아제비적아본질시건완전탈광후획득효원섬유시건,통과수궤수자표법분성3조(매조50개),분별침포우인공타액、함7％ MAE-DB단체적인공타액이급함2％록기정적인공타액중,경랭열순배5000화10 000차후,검측효원섬유시건랍신강도적변화,평개MAE-DB단체대결합형기질금속단백매적작용.투사전경관찰랭열순배후각조효원섬유시건적형태.결과 1 h 3％ MAE-DB단체대MMP-8활성적억제백분비위99.53％,현저고우억제제대조조(95.71％)(P＜0.05).랭열순배5000차화10 000차후,함7％ MAE-DB조효원섬유시건적랍신강도[분별위(10.66±2.12)화(8.89±1.89) MPa]분별현저고우인공타액조[분별위(7.43±2.27)화(5.46±1.76) MPa]화함2％록기정인공타액조[분별위(9.34±2.53)화(7.67 ±2.20) MPa] (P＜0.05).투사전경현시7％ MAE-DB조효원섬유미관결구완정,이인공타액조효원섬유미관결구문란.결론 쌍갑기병희선계안염단체MAE-DB가유효억제MMP-8적활성,보호효원섬유,강저효원섬유재노화과정중적매해.
Objective To evaluate the anti-matrix metalloproteinase (MMP) activity of a novel crosslinking quaternary ammonium methacrylates,2-methacryloxylethyl dodecylmethyl ammonium bromide (MAE-DB).Methods The effects of MAE-DB at different concentrations (1％,3％,5％,7％) on soluble matrix metalloproteinase-8 (MMP-8) were investigated using fluorescent assay kit.Readings were taken every 20 min for 3 h.The 1,10-phenanthroline provided by the assay kit served as control group.Demineralized dentin beams were randomly divided into three groups (n=50) and immersed in different solutions:artificial saliva,MAE-DB incorporated artificial saliva and chlorhexidine incorporated artificial saliva.After temperature cycling,the changes of ultimate tensile strength were measured to determine the effect of MAE-DB on the activity of matrix-bound endogenous matrix metalloproteinases.The morphology of dentin collagen fibrils in the three groups was observed via transmission electron microscopy (TEM).Results MAE-DB could effectively inhibit the activity of soluble MMP-8.The inhibition percentage of 3％ MAE-DB was 99.53％ after 1 h,and it was significantly higher than that of 1,10-phenanthroline (95.71％,P ＜0.05).After temperature cycling,the ultimate tensile strengths of MAE-DB groups were significantly higher than those of the artificial saliva groups and the chlorhexidine groups (P ＜ 0.05).TEM micrographs of MAE-DB group revealed that the microstructure of the collagen fibrillar was intact,while the fibrillar in the artificial saliva group was disrupted,indicating a protective function of MAE-DB on dentin collagen.Conclusions MAE-DB can inhibit the activity of MMP and protect dentin collagen from enzyme degradation.
