中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
6期
343-347
,共5页
细胞增殖%细胞分化%牙乳头%三氧化矿物凝聚体
細胞增殖%細胞分化%牙乳頭%三氧化礦物凝聚體
세포증식%세포분화%아유두%삼양화광물응취체
Cell proliferation%Cell differentiation%Dental papilla%Mineral trioxide aggregate
目的 研究不同质量浓度的三氧化矿物凝聚体(mineral trioxide aggregate,MTA)对大鼠牙乳头细胞(rat dental papilla cells,RDPC)增殖和分化能力的影响,探讨MTA在根尖诱导方面的作用.方法 组织块法原代培养RDPC并鉴定.制备不同质量浓度的MTA浸提液(0.002、0.02、0.2、2及20 g/L)并处理第3代RDPC 3 d,以普通培养液的RDPC作为空白对照组(每组6孔),用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法检测细胞增殖能力,筛选最适合细胞生长的质量浓度.用0.002 g/L MTA培养RDPC,以普通培养液的RDPC作为空白对照组,检测1、3、5、7 d RDPC的碱性磷酸酶(alkaline phosphatase,ALP)活性和1、3、5 d RDPC的Ⅰ型胶原含量.用方差分析对不同浓度MTA干预后RDPC的增殖能力进行统计学分析,用t检验对0.002 g/L MTA干预后RDPC分泌的ALP活性和Ⅰ型胶原含量进行统计学分析.结果 MTT法检测不同质量浓度MTA对RDPC增殖能力影响中,培养3d后的A值20 g/L组(0.092±0.011)显著低于空白对照组(0.249±0.006),差异有统计学意义(P＜0.01)；0.02和0.002 g/L组的A值(分别为0.267±0.005、0.276±0.006)均显著高于空白对照组(0.249±0.006),差异均有统计学意义(P＜0.01).ALP活性检测结果显示,MTA组3、5、7d的A值[分别为(0.217 ±0.008)、(0.253±0.005)和(0.279±0.004)]均显著高于空白对照组相应时间点的A值[分别为(0.166±0.006)、(0.221±0.006)、(0.242±0.004)],差异均有统计学意义(P＜0.01).3、5d的Ⅰ型胶原含量MTA组[分别为(78.46±2.72)、(90.73±3.08) μg/L]均显著高于相应时间点的空白对照组[分别为(66.75±3.08)、(74.27±3.50) μg/L],差异均有统计学意义(P＜0.01).结论 高浓度的MTA抑制RDPC的生长,低浓度的MTA可以促进RDPC的增殖和分化.
目的 研究不同質量濃度的三氧化礦物凝聚體(mineral trioxide aggregate,MTA)對大鼠牙乳頭細胞(rat dental papilla cells,RDPC)增殖和分化能力的影響,探討MTA在根尖誘導方麵的作用.方法 組織塊法原代培養RDPC併鑒定.製備不同質量濃度的MTA浸提液(0.002、0.02、0.2、2及20 g/L)併處理第3代RDPC 3 d,以普通培養液的RDPC作為空白對照組(每組6孔),用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法檢測細胞增殖能力,篩選最適閤細胞生長的質量濃度.用0.002 g/L MTA培養RDPC,以普通培養液的RDPC作為空白對照組,檢測1、3、5、7 d RDPC的堿性燐痠酶(alkaline phosphatase,ALP)活性和1、3、5 d RDPC的Ⅰ型膠原含量.用方差分析對不同濃度MTA榦預後RDPC的增殖能力進行統計學分析,用t檢驗對0.002 g/L MTA榦預後RDPC分泌的ALP活性和Ⅰ型膠原含量進行統計學分析.結果 MTT法檢測不同質量濃度MTA對RDPC增殖能力影響中,培養3d後的A值20 g/L組(0.092±0.011)顯著低于空白對照組(0.249±0.006),差異有統計學意義(P＜0.01)；0.02和0.002 g/L組的A值(分彆為0.267±0.005、0.276±0.006)均顯著高于空白對照組(0.249±0.006),差異均有統計學意義(P＜0.01).ALP活性檢測結果顯示,MTA組3、5、7d的A值[分彆為(0.217 ±0.008)、(0.253±0.005)和(0.279±0.004)]均顯著高于空白對照組相應時間點的A值[分彆為(0.166±0.006)、(0.221±0.006)、(0.242±0.004)],差異均有統計學意義(P＜0.01).3、5d的Ⅰ型膠原含量MTA組[分彆為(78.46±2.72)、(90.73±3.08) μg/L]均顯著高于相應時間點的空白對照組[分彆為(66.75±3.08)、(74.27±3.50) μg/L],差異均有統計學意義(P＜0.01).結論 高濃度的MTA抑製RDPC的生長,低濃度的MTA可以促進RDPC的增殖和分化.
목적 연구불동질량농도적삼양화광물응취체(mineral trioxide aggregate,MTA)대대서아유두세포(rat dental papilla cells,RDPC)증식화분화능력적영향,탐토MTA재근첨유도방면적작용.방법 조직괴법원대배양RDPC병감정.제비불동질량농도적MTA침제액(0.002、0.02、0.2、2급20 g/L)병처리제3대RDPC 3 d,이보통배양액적RDPC작위공백대조조(매조6공),용갑기새서기사서(methyl thiazolyl tetrazolium,MTT)비색법검측세포증식능력,사선최괄합세포생장적질량농도.용0.002 g/L MTA배양RDPC,이보통배양액적RDPC작위공백대조조,검측1、3、5、7 d RDPC적감성린산매(alkaline phosphatase,ALP)활성화1、3、5 d RDPC적Ⅰ형효원함량.용방차분석대불동농도MTA간예후RDPC적증식능력진행통계학분석,용t검험대0.002 g/L MTA간예후RDPC분비적ALP활성화Ⅰ형효원함량진행통계학분석.결과 MTT법검측불동질량농도MTA대RDPC증식능력영향중,배양3d후적A치20 g/L조(0.092±0.011)현저저우공백대조조(0.249±0.006),차이유통계학의의(P＜0.01)；0.02화0.002 g/L조적A치(분별위0.267±0.005、0.276±0.006)균현저고우공백대조조(0.249±0.006),차이균유통계학의의(P＜0.01).ALP활성검측결과현시,MTA조3、5、7d적A치[분별위(0.217 ±0.008)、(0.253±0.005)화(0.279±0.004)]균현저고우공백대조조상응시간점적A치[분별위(0.166±0.006)、(0.221±0.006)、(0.242±0.004)],차이균유통계학의의(P＜0.01).3、5d적Ⅰ형효원함량MTA조[분별위(78.46±2.72)、(90.73±3.08) μg/L]균현저고우상응시간점적공백대조조[분별위(66.75±3.08)、(74.27±3.50) μg/L],차이균유통계학의의(P＜0.01).결론 고농도적MTA억제RDPC적생장,저농도적MTA가이촉진RDPC적증식화분화.
Objective To investigate the effects of mineral trioxide aggregate (MTA) on the proliferation and differentiation of rat dental papilla cells(RDPC).Methods RDPC were cultured by tissue block method and identified.RDPC of the third passage were cultured with material extract fluid containing different mass concentrations of MTA (0.002,0.02,0.2,2 and 20 g/L) for 3 d,those cultured with routine culture fluid served as control group.The proliferation-related parameters were measured by methyl thiazolyl tetrazolium(MTT) assay.RDPC were cultured with material extract fluids containing 0.002 g/L MTA,those cultured with routine culture fluid served as control group,the activity of alkaline phosphatase(ALP) at 1,3,5,7 d and the level of collagen Ⅰ at 1,3,5 d were detected.Results MTT results showed that the A value of RDPC of group 20 g/L(0.092 ±0.011) was less than that of the control group(0.249 ±0.006)at 3 d (P ＜ 0.01),the A value of RDPC of group 0.02 g/L (0.267 ± 0.005) and 0.002 g/L (0.276 ±0.006) were more than that of the control group (0.249 ± 0.006) at 3 d (P ＜ 0.01).ALP detection proved that ALP activity of MTA at 3 d (0.217 ± 0.008),5 d (0.253 ± 0.005),7 d (0.279 ± 0.004) were more than that of the control group at 3 d(0.166±0.006),5 d(0.221 ±0.006),7 d(0.242 ±0.004)(P ＜0.01).Collagen Ⅰ detection showed that the level of collagen Ⅰ of MTA at 3 d[(78.46 ±2.72) μg/L],5 d [(90.73 ± 3.08) μg/L] were more than that of the control group at 3 d [(66.75 ± 3.08) μg/L],[5 d(74.27 ± 3.50) μg/L] (P ＜ 0.01).Conclusions MTA of high concentrations can significantly inhibit cell growth,and of low concentrations can promote cells proliferation and differentiation.
