CAJ | 학술논문

目的分析微RNA-31(microRNA)在口腔鳞状细胞癌中的表达及其与临床病理特征的关系.方法分别取口腔鳞状细胞癌手术标本62例及其癌旁正常口腔黏膜组织,提取总RNA,采用茎环反转录实时定量PCR方法检测微RNA-31在口腔鳞状细胞癌及其癌旁正常黏膜中的表达量,并分析其与临床病理特征的关系.对性别、年龄和肿瘤分化行t检验,肿瘤部位、大小、TNM分期和淋巴结转移行方差分析.结果与癌旁组织比较,微RNA-31在口腔鳞状细胞癌组织中表达显著上调(表达值中位数均为1.87,P＜0.05),微RNA-31的表达与淋巴结转移状态(P＜0.05)及肿瘤细胞分化程度相关(P＜0.05),与性别、年龄、肿瘤淋巴结转移分期、肿瘤大小、肿瘤部位无关.受试者工作特征曲线(receiver operator characteristic curve,ROC)分析显示,微RNA-31评价口腔鳞状细胞癌细胞分化程度的特异性和敏感性分别为70.4％、89.5％.结论微RNA-31在口腔鳞状细胞癌中的表达上调可能与口腔鳞状细胞癌的发生发展有关.
목적 분석미RNA-31(microRNA)재구강린상세포암중적표체급기여림상병리특정적관계.방법 분별취구강린상세포암수술표본62례급기암방정상구강점막조직,제취총RNA,채용경배반전록실시정량PCR방법검측미RNA-31재구강린상세포암급기암방정상점막중적표체량,병분석기여림상병리특정적관계.대성별、년령화종류분화행t검험,종류부위、대소、TNM분기화림파결전이행방차분석.결과 여암방조직비교,미RNA-31재구강린상세포암조직중표체현저상조(표체치중위수균위1.87,P＜0.05),미RNA-31적표체여림파결전이상태(P＜0.05)급종류세포분화정도상관(P＜0.05),여성별、년령、종류림파결전이분기、종류대소、종류부위무관.수시자공작특정곡선(receiver operator characteristic curve,ROC)분석현시,미RNA-31평개구강린상세포암세포분화정도적특이성화민감성분별위70.4％、89.5％.결론 미RNA-31재구강린상세포암중적표체상조가능여구강린상세포암적발생발전유관.
Objective To investigate the expression of microRNA-31 and its association with clinicopathologic features in oral squamous cell carcinoma (OSCC).Methods The expression level of microRNA-31 in 62 cases of OSCC and matched non-tumor adjacent tissue specimens was examined using stem-loop real-time PCR.The relationship between the expression of microRNA-31 and its clinicopathologic features of OSCC was analyzed.Results The expression of microRNA-31 was significantly higher in the tumor tissues than that in the adjacent tissues (P ＜ 0.05).Up-regulated microRNA-31 expression was associated with the lymph node metastasis (P ＜ 0.05) and cell differentiation (P ＜ 0.05) in OSCC patients.No significant association was found between the expression of microRNA-31 and gender,age,lymph node metastasis,tumor size and location.Receiver operator characteristic curve (ROC) of microRNA-31 about cell differentiation resulted in a diagnostic sensitivity of 70.4％ and specificity of 89.5％.Conclusions The up-regulated level of microRNA-31 expression may be related to the pathogenesis of OSCC.