中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
9期
523-528
,共6页
赵姝亚%王旭霞%刘宛鑫%董瑞%李静%张君
趙姝亞%王旭霞%劉宛鑫%董瑞%李靜%張君
조주아%왕욱하%류완흠%동서%리정%장군
二膦酸盐类%牙根吸收%破骨细胞%骨质吸收
二膦痠鹽類%牙根吸收%破骨細胞%骨質吸收
이련산염류%아근흡수%파골세포%골질흡수
Biphosphonates%Root resorption%Osteoclasts%Bone resorption
目的 比较抗代谢性骨病药物帕米膦酸钠和伊班膦酸钠对大鼠正畸牙根吸收的影响,为正畸临床预防牙根吸收提供参考.方法 选择72只6周龄无特定病原体(specific pathogen free,SPF)级健康雌性Wistar大鼠建立正畸牙齿移动动物模型,按随机数字表随机分为对照组、帕米膦酸钠组与伊班膦酸钠组,每组24只,分别注射生理盐水(0.9％ NaCl溶液)、0.5 mmol/L帕米膦酸钠溶液和0.5 mmol/L伊班膦酸钠溶液各50μl.实验第3、7、14天时每组分别处死8只大鼠行组织切片观察,进行破牙骨质细胞计数、破骨细胞分化因子(osteoclast differentiation factor,ODF)检测及牙根吸收指数分析,使用PASW Statistics18软件进行单因素方差分析和LSD比较.结果 各组第3天破牙骨质细胞数、ODF表达及牙根吸收指数差异均无统计学意义(P＞0.05)；第7和14天破牙骨质细胞数、ODF表达及牙根吸收指数帕米膦酸钠组[(2.675±0.002)个、(0.1683±0.0007)、(0.103±0.003)和(3.886 ±0.048)个、(0.1873 ±0.0014)、(0.283 ±0.001)]和伊班膦酸钠组[(2.601±0.001)个、(0.1634±0.0010)、(0.099 ±0.002)和(3.754±0.019)个、(0.1818±0.0016)、(0.281 ±0.001)]均显著小于相同时间点对照组[(2.810 ±0.001)个、(0.1792±0.0008)、(0.120±0.001)和(4.800±0.001)个、(0.2060 ±0.0007)、(0.401±0.001)](P＜0.05)；相同时间点帕米膦酸钠与伊班膦酸钠组各指标的组间差异均无统计学意义(P＞0.05).结论 帕米膦酸钠与伊班膦酸钠对大鼠正畸牙根吸收的影响基本一致,均可减轻牙根吸收的程度.
目的 比較抗代謝性骨病藥物帕米膦痠鈉和伊班膦痠鈉對大鼠正畸牙根吸收的影響,為正畸臨床預防牙根吸收提供參攷.方法 選擇72隻6週齡無特定病原體(specific pathogen free,SPF)級健康雌性Wistar大鼠建立正畸牙齒移動動物模型,按隨機數字錶隨機分為對照組、帕米膦痠鈉組與伊班膦痠鈉組,每組24隻,分彆註射生理鹽水(0.9％ NaCl溶液)、0.5 mmol/L帕米膦痠鈉溶液和0.5 mmol/L伊班膦痠鈉溶液各50μl.實驗第3、7、14天時每組分彆處死8隻大鼠行組織切片觀察,進行破牙骨質細胞計數、破骨細胞分化因子(osteoclast differentiation factor,ODF)檢測及牙根吸收指數分析,使用PASW Statistics18軟件進行單因素方差分析和LSD比較.結果 各組第3天破牙骨質細胞數、ODF錶達及牙根吸收指數差異均無統計學意義(P＞0.05)；第7和14天破牙骨質細胞數、ODF錶達及牙根吸收指數帕米膦痠鈉組[(2.675±0.002)箇、(0.1683±0.0007)、(0.103±0.003)和(3.886 ±0.048)箇、(0.1873 ±0.0014)、(0.283 ±0.001)]和伊班膦痠鈉組[(2.601±0.001)箇、(0.1634±0.0010)、(0.099 ±0.002)和(3.754±0.019)箇、(0.1818±0.0016)、(0.281 ±0.001)]均顯著小于相同時間點對照組[(2.810 ±0.001)箇、(0.1792±0.0008)、(0.120±0.001)和(4.800±0.001)箇、(0.2060 ±0.0007)、(0.401±0.001)](P＜0.05)；相同時間點帕米膦痠鈉與伊班膦痠鈉組各指標的組間差異均無統計學意義(P＞0.05).結論 帕米膦痠鈉與伊班膦痠鈉對大鼠正畸牙根吸收的影響基本一緻,均可減輕牙根吸收的程度.
목적 비교항대사성골병약물파미련산납화이반련산납대대서정기아근흡수적영향,위정기림상예방아근흡수제공삼고.방법 선택72지6주령무특정병원체(specific pathogen free,SPF)급건강자성Wistar대서건립정기아치이동동물모형,안수궤수자표수궤분위대조조、파미련산납조여이반련산납조,매조24지,분별주사생리염수(0.9％ NaCl용액)、0.5 mmol/L파미련산납용액화0.5 mmol/L이반련산납용액각50μl.실험제3、7、14천시매조분별처사8지대서행조직절편관찰,진행파아골질세포계수、파골세포분화인자(osteoclast differentiation factor,ODF)검측급아근흡수지수분석,사용PASW Statistics18연건진행단인소방차분석화LSD비교.결과 각조제3천파아골질세포수、ODF표체급아근흡수지수차이균무통계학의의(P＞0.05)；제7화14천파아골질세포수、ODF표체급아근흡수지수파미련산납조[(2.675±0.002)개、(0.1683±0.0007)、(0.103±0.003)화(3.886 ±0.048)개、(0.1873 ±0.0014)、(0.283 ±0.001)]화이반련산납조[(2.601±0.001)개、(0.1634±0.0010)、(0.099 ±0.002)화(3.754±0.019)개、(0.1818±0.0016)、(0.281 ±0.001)]균현저소우상동시간점대조조[(2.810 ±0.001)개、(0.1792±0.0008)、(0.120±0.001)화(4.800±0.001)개、(0.2060 ±0.0007)、(0.401±0.001)](P＜0.05)；상동시간점파미련산납여이반련산납조각지표적조간차이균무통계학의의(P＞0.05).결론 파미련산납여이반련산납대대서정기아근흡수적영향기본일치,균가감경아근흡수적정도.
Objective To compare the effects of pamidronate and ibandronate on orthodontic root resorption.Methods Seventy-two 6-week-old female specific pathogen free(SPF) Wistar rats were selected to establish models for orthodontic tooth movement.The rats were randomly divided into three groups:the control group(C group),pamidronate group (Pm group) and ibandronate group (Ib group).0.9％ normal saline,0.5 mmol/L pamidronate and 0.5 mmol/L ibandronate were injected every 3 days.The rats were executed in batch on the 3rd,7th and 14th day to make tissue sections.All statistical analysis was performed using the PASW Statistics 18 software package.Results On the 7th and 14th day,the amount of cementoclast,the expression of osteoclast differentiation factor (ODF) and root resorption index were significantly lower in Pm group[the 7th day:(2.675 ± 0.002),(0.1683 ± 0.0007),(0.103 ± 0.003) ;the 14th day:(3.886 ± 0.048),(0.1873 ± 0.0014),(0.283 ± 0.001)] and Ib groups [the 7th day:(2.601 ±0.001),(0.1634 ± 0.0010),(0.099 ± 0.002) ; the 14th day:(3.754 ± 0.019),(0.1818 ± 0.0016),(0.281 ±0.001)] than in C group[the 7th day:(2.810±0.001),(0.1792±0.0008),(0.120±0.001) ;the 14th day:(4.800 ± 0.001),(0.2060 ± 0.0007),(0.401 ± 0.001)] (P ＜ 0.05).However,no significant difference was found between Pm and Ib groups on the 3rd,7th and 14th day(P ＞ 0.05).Conclusions Both pamidronate and ibandronate could inhibit orthodontic root resorption.
