中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
z1期
23-28
,共6页
翁金龙%赵河川%孙凤%陈霄迟%徐韬
翁金龍%趙河川%孫鳳%陳霄遲%徐韜
옹금룡%조하천%손봉%진소지%서도
氟化物,外用%龋齿%变性梯度凝胶电泳%菌群多样性
氟化物,外用%齲齒%變性梯度凝膠電泳%菌群多樣性
불화물,외용%우치%변성제도응효전영%균군다양성
Fluorides,topical%Dental caries%Denaturing gradient gel electrophoresis%Flora diversity
目的 观察临床应用1.23％氟化泡沫对儿童牙面菌斑微生物组成的影响.方法 选取北京市海淀区幼儿园的10名学龄前儿童,平均年龄3.7岁；常规口腔检查,记录龋失补牙面数；样本采自1.23％氟化泡沫干预前后1个月内不同时间(基线及涂氟后第1、2、3、4、7、10、15、21、30天)相同牙面的菌斑；应用PCR-变性梯度凝胶电泳技术表达菌斑微生物DNA图谱,通过GelComparⅡ分析软件解析菌斑微生物的群体相关性,采用Mann-Whitney U检验分析微生物多样性指数(ShannonWeiner指数),通过聚类统计分析样本微生物群落不同时间点的相似性及种群聚类分析.结果 10名儿童基线菌斑微生物DNA的条带数为26.9±2.9,使用氟化泡沫干预3d后菌斑微生物DNA条带数下降至20.1 ±3.8,与基线相比差异有统计学意义(P＜0.05)；Shannon-Weiner指数在基线为3.18±0.31,干预3d后为2.92 ±0.28,二者差异有统计学意义(P=0.04).10名儿童的微生物电泳图谱戴斯系数(Dice coefficient)和个体微生物群落聚类分析表明,氟化泡沫干预后菌斑微生物种群结构发生变化,但1个月后菌斑结构与基线相似.结论 1.23％氟化泡沫对儿童牙面菌斑微生物在一定时间内有明显的抑制作用；应用氟化泡沫后菌斑微生物种群结构经历了复杂的变化后还能恢复至原有状态.
目的 觀察臨床應用1.23％氟化泡沫對兒童牙麵菌斑微生物組成的影響.方法 選取北京市海澱區幼兒園的10名學齡前兒童,平均年齡3.7歲；常規口腔檢查,記錄齲失補牙麵數；樣本採自1.23％氟化泡沫榦預前後1箇月內不同時間(基線及塗氟後第1、2、3、4、7、10、15、21、30天)相同牙麵的菌斑；應用PCR-變性梯度凝膠電泳技術錶達菌斑微生物DNA圖譜,通過GelComparⅡ分析軟件解析菌斑微生物的群體相關性,採用Mann-Whitney U檢驗分析微生物多樣性指數(ShannonWeiner指數),通過聚類統計分析樣本微生物群落不同時間點的相似性及種群聚類分析.結果 10名兒童基線菌斑微生物DNA的條帶數為26.9±2.9,使用氟化泡沫榦預3d後菌斑微生物DNA條帶數下降至20.1 ±3.8,與基線相比差異有統計學意義(P＜0.05)；Shannon-Weiner指數在基線為3.18±0.31,榦預3d後為2.92 ±0.28,二者差異有統計學意義(P=0.04).10名兒童的微生物電泳圖譜戴斯繫數(Dice coefficient)和箇體微生物群落聚類分析錶明,氟化泡沫榦預後菌斑微生物種群結構髮生變化,但1箇月後菌斑結構與基線相似.結論 1.23％氟化泡沫對兒童牙麵菌斑微生物在一定時間內有明顯的抑製作用；應用氟化泡沫後菌斑微生物種群結構經歷瞭複雜的變化後還能恢複至原有狀態.
목적 관찰림상응용1.23％불화포말대인동아면균반미생물조성적영향.방법 선취북경시해정구유인완적10명학령전인동,평균년령3.7세；상규구강검사,기록우실보아면수；양본채자1.23％불화포말간예전후1개월내불동시간(기선급도불후제1、2、3、4、7、10、15、21、30천)상동아면적균반；응용PCR-변성제도응효전영기술표체균반미생물DNA도보,통과GelComparⅡ분석연건해석균반미생물적군체상관성,채용Mann-Whitney U검험분석미생물다양성지수(ShannonWeiner지수),통과취류통계분석양본미생물군락불동시간점적상사성급충군취류분석.결과 10명인동기선균반미생물DNA적조대수위26.9±2.9,사용불화포말간예3d후균반미생물DNA조대수하강지20.1 ±3.8,여기선상비차이유통계학의의(P＜0.05)；Shannon-Weiner지수재기선위3.18±0.31,간예3d후위2.92 ±0.28,이자차이유통계학의의(P=0.04).10명인동적미생물전영도보대사계수(Dice coefficient)화개체미생물군락취류분석표명,불화포말간예후균반미생물충군결구발생변화,단1개월후균반결구여기선상사.결론 1.23％불화포말대인동아면균반미생물재일정시간내유명현적억제작용；응용불화포말후균반미생물충군결구경력료복잡적변화후환능회복지원유상태.
Objective To investigate the influence of 1.23％ fluoride foam on microflora in dental plaque of children.Methods Ten children whose average age was 3.7 years from a kindergarten in Beijing city were selected in this study.Oral examinations were conducted and decayed,missing,filled surface (dmfs) value were recorded at baseline.Subjects were applied with 1.23％ fluoride foam.The buccal plaque samples from bilateral maxillary primary molars (V IV | IV V) were collected before and after the fluoride application at baseline,1st,2nd,3rd,4th,7th,10th,15th,21th,30th day.The total microbial genomic DNA of the plaque was isolated.PCR was performed with a set of universal bacterial 16S rDNA primers.The PCR amplified 16S rDNA fragments were separated by denaturing gradient gel electrophoresis (DGGE).The relationship between microflora was assessed by comparing the PCR-DGGE fingerprinting profiles.Results The mean species richness of the bacterial population was 26.9 ± 2.9 at baseline,and fell to 20.1 ± 3.8 after three days of using fluoride foam.The difference was statistically significant (P ＜ 0.05).The overall diversity of plaque samples as measured by the Shannon-Weiner index was 3.18 ±0.31 at baseline and 2.92 ± 0.28 at 3rd day.The difference was statistically significant (P =0.04 by the Mann-Whitney U test).The microbial diversity was changed after the fluoride application by Dice coefficient and clustering analysis.After one month,the microbial diversity was similar to the baseline.Conclusions The results suggest that 1.23％ fluoride foam has obviously inhibiting effects on microbial diversity in dental plaque of children during a period of time.The microbial diversity and complexity of the microbial biota in dental plaque were changed after fluoride application and was able to return to original state.
