中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
z1期
58-63
,共6页
林雪彩%孙红英%甄月香%张慧
林雪綵%孫紅英%甄月香%張慧
림설채%손홍영%견월향%장혜
扁平苔癣,口腔%受体,糖皮质激素%NF-κB%炎症反应
扁平苔癬,口腔%受體,糖皮質激素%NF-κB%炎癥反應
편평태선,구강%수체,당피질격소%NF-κB%염증반응
Lichen planus,oral%Glucocorticoid receptor%Nuclear factor-kappa B%Inflammation
目的 研究糖皮质激素受体(glucocorticoid receptor,GR)及核因子κB(nuclear factor-κB,NF-κB)在口腔扁平苔藓(oral lichen planus,OLP)中的作用,探讨GR及NF-κB在OLP发生中的相关性.方法 采用蛋白质印迹法和实时定量反转录PCR(reverse transcription-PCR,RT-PCR)对GRα、GRβ、p65、p50在32例OLP患者局部病损口腔黏膜(OLP组,患者均来自复旦大学附属华山医院口腔科口腔黏膜病门诊)和12例健康口腔黏膜(健康对照组,均选自复旦大学附属华山医院口腔科门诊阻生牙拔除术必需切除的口腔黏膜)中的表达情况进行分析,并采用相关性分析研究GR和NF-κB在OLP发生中的相关性.结果 GRα、GRβ、p65、p50蛋白在OLP组和健康对照组中均有表达.OLP组GRα mRNA的表达水平(0.85±0.04)显著低于健康对照组(1.03±0.03),差异有统计学意义(P =0.000)；GRβ mRNA的表达水平(1.02±0.06)与健康对照组(0.98±0.03)相比差异无统计学意义(P=0.069)；p65 mRNA和p50 mRNA的表达水平(1.30±0.14、1.21±0.08)均显著高于健康对照组(0.99±0.02、1.03±0.03),差异均有统计学意义(P =0.000,P=0.000).GRα与p65在OLP中的表达水平呈负相关(r=-0.428.P=0.015)；GRα与p50在OLP中的表达水平也呈负相关(r=-0.536.P=0.002).结论 GRα在OLP中表达下调,可能与OLP慢性炎症迁延不愈和糖皮质激素的疗效有关；GR与NF-κB在OLP炎症反应中可能具有拮抗作用.
目的 研究糖皮質激素受體(glucocorticoid receptor,GR)及覈因子κB(nuclear factor-κB,NF-κB)在口腔扁平苔蘚(oral lichen planus,OLP)中的作用,探討GR及NF-κB在OLP髮生中的相關性.方法 採用蛋白質印跡法和實時定量反轉錄PCR(reverse transcription-PCR,RT-PCR)對GRα、GRβ、p65、p50在32例OLP患者跼部病損口腔黏膜(OLP組,患者均來自複旦大學附屬華山醫院口腔科口腔黏膜病門診)和12例健康口腔黏膜(健康對照組,均選自複旦大學附屬華山醫院口腔科門診阻生牙拔除術必需切除的口腔黏膜)中的錶達情況進行分析,併採用相關性分析研究GR和NF-κB在OLP髮生中的相關性.結果 GRα、GRβ、p65、p50蛋白在OLP組和健康對照組中均有錶達.OLP組GRα mRNA的錶達水平(0.85±0.04)顯著低于健康對照組(1.03±0.03),差異有統計學意義(P =0.000)；GRβ mRNA的錶達水平(1.02±0.06)與健康對照組(0.98±0.03)相比差異無統計學意義(P=0.069)；p65 mRNA和p50 mRNA的錶達水平(1.30±0.14、1.21±0.08)均顯著高于健康對照組(0.99±0.02、1.03±0.03),差異均有統計學意義(P =0.000,P=0.000).GRα與p65在OLP中的錶達水平呈負相關(r=-0.428.P=0.015)；GRα與p50在OLP中的錶達水平也呈負相關(r=-0.536.P=0.002).結論 GRα在OLP中錶達下調,可能與OLP慢性炎癥遷延不愈和糖皮質激素的療效有關；GR與NF-κB在OLP炎癥反應中可能具有拮抗作用.
목적 연구당피질격소수체(glucocorticoid receptor,GR)급핵인자κB(nuclear factor-κB,NF-κB)재구강편평태선(oral lichen planus,OLP)중적작용,탐토GR급NF-κB재OLP발생중적상관성.방법 채용단백질인적법화실시정량반전록PCR(reverse transcription-PCR,RT-PCR)대GRα、GRβ、p65、p50재32례OLP환자국부병손구강점막(OLP조,환자균래자복단대학부속화산의원구강과구강점막병문진)화12례건강구강점막(건강대조조,균선자복단대학부속화산의원구강과문진조생아발제술필수절제적구강점막)중적표체정황진행분석,병채용상관성분석연구GR화NF-κB재OLP발생중적상관성.결과 GRα、GRβ、p65、p50단백재OLP조화건강대조조중균유표체.OLP조GRα mRNA적표체수평(0.85±0.04)현저저우건강대조조(1.03±0.03),차이유통계학의의(P =0.000)；GRβ mRNA적표체수평(1.02±0.06)여건강대조조(0.98±0.03)상비차이무통계학의의(P=0.069)；p65 mRNA화p50 mRNA적표체수평(1.30±0.14、1.21±0.08)균현저고우건강대조조(0.99±0.02、1.03±0.03),차이균유통계학의의(P =0.000,P=0.000).GRα여p65재OLP중적표체수평정부상관(r=-0.428.P=0.015)；GRα여p50재OLP중적표체수평야정부상관(r=-0.536.P=0.002).결론 GRα재OLP중표체하조,가능여OLP만성염증천연불유화당피질격소적료효유관；GR여NF-κB재OLP염증반응중가능구유길항작용.
Objective To examine the expression and correlation of glucocorticoid receptors (GR)and nuclear factor-κB(NF-κB) in oral lichen planus(OLP).Methods Thirty-two OLP cases and 12 normal oral mucosa specimens were included.The oral mucosae were examined for GR and NF-κB by Westernblotting and real time reverse transcription-PCR(RT-PCR).The correlation between GR and NF-κB in OLP was analyzed by correlation analysis.Results The proteins of GRα,GRβ,p65 and p50 were expressed in both OLP and control group.The expression of GRα mRNA in the OLP was lower than that in control (0.85 ±0.04 in OLP and 1.03 ± 0.03 in control,P =0.000).But there was no difference of GRβ mRNA between OLP and normal samples (1.02 ± 0.06 in OLP and 0.98 ± 0.03 in control,P =0.069).The expression of p65 and p50 mRNA in the OLP both were higher than that in control(1.30 ±0.14 in OLP and 0.99 ±0.02 in control of p65,1.21 ± 0.08 in OLP and 1.03 ± 0.03 in control of p50,both P =0.000).A negative correlation was noted between GRα and p65 in OLP(r =-0.428,P =0.015),and the same correlation found between GRα and p50 (r =-0.536,P =0.002).Conclusions Low expression of GRα may contribute to the protracted course of inflammation and the efficacy of glucocorticoid in OLP.There is an antagonism between GRα and NF-κB in the inflammation of OLP.
