中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
z1期
64-67
,共4页
口腔溃疡%动物,实验%超氧化物歧化酶%丙二醛%辅酶Q10
口腔潰瘍%動物,實驗%超氧化物歧化酶%丙二醛%輔酶Q10
구강궤양%동물,실험%초양화물기화매%병이철%보매Q10
Oral ulcer%Animals,laboratory%Superoxide dismutase%Malondialdehyde%Coenzyme Q10
目的 通过应用辅酶Q10后实验性大鼠口腔溃疡组织中超氧化物歧化酶(superoxide dismutase,SOD)及丙二醛(malondialdehyde)的含量测定,评价辅酶Q10对大鼠口腔溃疡的影响,以期为口腔溃疡的治疗提供新方法.方法 将15只SD大鼠利用SPSS软件随机分为3组:正常对照组、模型对照组及辅酶Q10组,每组5只.正常对照组不作处理,其他两组利用冰醋酸法建立口腔溃疡动物模型,辅酶Q10组每天以辅酶Q10灌胃,其他两组以等体积生理盐水灌胃,用药13 d,观察并记录口腔溃疡的愈合情况；于实验13 d后处死动物切取相应黏膜组织,分光光度法测量组织匀浆中SOD及丙二醛的含量.结果辅酶Q10组溃疡平均愈合时间[(9.4±1.1)d]显著短于模型对照组[(12.0±1.6)d],且前者SOD含量[(114.81±3.69) U/mg]显著高于模型对照组[(89.66±12.50) U/mg],丙二醛含量[(1.83 ±0.13) μmol/g]显著低于模型对照组[(2.26 ±0.13) μmol/g],差异均有统计学意义(P＜0.05)；正常对照组SOD含量[(131.01 ±16.09) U/mg]显著高于其他两组,丙二醛含量[(1.31 ±0.15) μmol/g]显著低于其他两组,差异均有统计学意义(P＜0.05).结论 辅酶Q10可以增强组织中清除自由基和抗氧化的能力,对促进上皮组织修复加速溃疡愈合有积极意义.
目的 通過應用輔酶Q10後實驗性大鼠口腔潰瘍組織中超氧化物歧化酶(superoxide dismutase,SOD)及丙二醛(malondialdehyde)的含量測定,評價輔酶Q10對大鼠口腔潰瘍的影響,以期為口腔潰瘍的治療提供新方法.方法 將15隻SD大鼠利用SPSS軟件隨機分為3組:正常對照組、模型對照組及輔酶Q10組,每組5隻.正常對照組不作處理,其他兩組利用冰醋痠法建立口腔潰瘍動物模型,輔酶Q10組每天以輔酶Q10灌胃,其他兩組以等體積生理鹽水灌胃,用藥13 d,觀察併記錄口腔潰瘍的愈閤情況；于實驗13 d後處死動物切取相應黏膜組織,分光光度法測量組織勻漿中SOD及丙二醛的含量.結果輔酶Q10組潰瘍平均愈閤時間[(9.4±1.1)d]顯著短于模型對照組[(12.0±1.6)d],且前者SOD含量[(114.81±3.69) U/mg]顯著高于模型對照組[(89.66±12.50) U/mg],丙二醛含量[(1.83 ±0.13) μmol/g]顯著低于模型對照組[(2.26 ±0.13) μmol/g],差異均有統計學意義(P＜0.05)；正常對照組SOD含量[(131.01 ±16.09) U/mg]顯著高于其他兩組,丙二醛含量[(1.31 ±0.15) μmol/g]顯著低于其他兩組,差異均有統計學意義(P＜0.05).結論 輔酶Q10可以增彊組織中清除自由基和抗氧化的能力,對促進上皮組織脩複加速潰瘍愈閤有積極意義.
목적 통과응용보매Q10후실험성대서구강궤양조직중초양화물기화매(superoxide dismutase,SOD)급병이철(malondialdehyde)적함량측정,평개보매Q10대대서구강궤양적영향,이기위구강궤양적치료제공신방법.방법 장15지SD대서이용SPSS연건수궤분위3조:정상대조조、모형대조조급보매Q10조,매조5지.정상대조조불작처리,기타량조이용빙작산법건립구강궤양동물모형,보매Q10조매천이보매Q10관위,기타량조이등체적생리염수관위,용약13 d,관찰병기록구강궤양적유합정황；우실험13 d후처사동물절취상응점막조직,분광광도법측량조직균장중SOD급병이철적함량.결과보매Q10조궤양평균유합시간[(9.4±1.1)d]현저단우모형대조조[(12.0±1.6)d],차전자SOD함량[(114.81±3.69) U/mg]현저고우모형대조조[(89.66±12.50) U/mg],병이철함량[(1.83 ±0.13) μmol/g]현저저우모형대조조[(2.26 ±0.13) μmol/g],차이균유통계학의의(P＜0.05)；정상대조조SOD함량[(131.01 ±16.09) U/mg]현저고우기타량조,병이철함량[(1.31 ±0.15) μmol/g]현저저우기타량조,차이균유통계학의의(P＜0.05).결론 보매Q10가이증강조직중청제자유기화항양화적능력,대촉진상피조직수복가속궤양유합유적겁의의.
Objective To evaluate the curative effect of coenzyme Q10 in rat oral ulcer by measuring the level of superoxide dismutase (SOD) and malondialdehyde (MDA) in oral ulcer tissues of experimental rats.Methods Fifteen SD rats were randomly divided into three groups,normal control group,model control group,coenzyme Q10 group (n =5).Normal control group did not receive treatment,while oral ulcer in other groups was created with glacial acetic acid method.Coenzyme Q10 group was daily intragastric administrated with coenzyme Q10,and the other two groups with an equal volume of saline for 13 days.The oral ulcer was observed and recorded.Thirteen days later,the animals were sacrificed and ulcer samples and normal mucosa were dissected.The contents of SOD and MDA in tissue homogenates were measured by photomery.Results The average cure period [(9.4 ± 1.1) d] in coenzyme Q10 group was significantly shorter than that of model control group [(12.0 ± 1.6) d].The SOD level [(114.81 ± 3.69) U/mg]in coenzyme Q10 group was significantly higher than that in model control group [(89.66 ± 12.50) U/mg],and MDA level in coenzyme Q10 group [(1.83 ±0.13) μmol/g] was lower than that in model control group [(2.26 ± 0.13) μmol/g] (P ＜ 0.05).Compared with the other two groups,the normal control group had a higher activity of SOD[(131.01 ± 16.09) U/mg] and a lower content of MDA [(1.31 ± 0.15) μmol/g] (P ＜ 0.05).Conclusions Coenzyme Q10 may promote ulcer healing by enhancing the capacity of free radical scavenging and antioxidant.
