中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
z1期
77-82
,共6页
黄伟琨%管晓燕%刘建国%孔宁静%范芹%张迪
黃偉琨%管曉燕%劉建國%孔寧靜%範芹%張迪
황위곤%관효연%류건국%공저정%범근%장적
脂多糖类%细胞因子类%银杏叶提取物%人牙周膜细胞
脂多糖類%細胞因子類%銀杏葉提取物%人牙週膜細胞
지다당류%세포인자류%은행협제취물%인아주막세포
Lipopolysaccharides%Cytokines%Ginkgo biloba extract%Periodontal ligament cells
目的 观察银杏叶提取物(Ginkgo biloba extract,GBE)对脂多糖(lipopolysaccharide,LPS)作用下人牙周膜细胞(periodental ligament cell,PDLC)增殖及分泌白细胞介素(interleukin,IL)6、IL-1β及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的影响,为GBE在牙周病防治中的应用提供依据.方法 选取2010年5至7月在遵义医学院附属口腔医院口腔颌面外科门诊因正畸拔除的10例11 ～15岁患者的20颗前磨牙,采用改良组织块培养法体外原代培养人PDLC,实验共分5组:①阴性对照组,含10 ml/L胎牛血清的达尔伯克改良伊格尔培养基(Dulbecco's modified Eagle's medium,DMEM)培养液；②LPS组,含10 ml/L胎牛血清的DMEM培养液+100 mg/L LPS；③LPS+0.1 g/L GBE组,含10 ml/L胎牛血清的DMEM培养液+100 mg/L LPS+0.1g/L GBE；④LPS+0.01 g/L GBE组,含10 ml/L胎牛血清的DMEM培养液+100 mg/L LPS +0.01 g/L GBE;⑤LPS+地塞米松组,含10 ml/L胎牛血清的DMEM培养液+100 mg/L LPS+2 mg/L地塞米松.甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法测定GBE对LPS作用下人PDLC活性的影响,酶联免疫吸附测定法测定各组IL-6、IL-1β及TNF-α的含量,对所得结果分别进行单因素方差分析,采用LSD-t检验进行组间两两比较,检验水准为双侧α=0.05.结果 实验12、24、48及72 h,LPS+0.1 g/L GBE组的吸光度值(分别为0.30±0.03、0.33±0.02、0.34 ±0.02及0.35 ±0.02)均显著高于LPS组(分别为0.20±0.03、0.20±0.02、0.22±0.04及0.24±0.02)(P＜0.05),PDLC IL-6、IL-1 β及TNF-α的分泌量均显著低于LPS组(P＜0.05)；实验12、24、48及72 h,LPS+0.01 g/L GBE组吸光度值(分别为0.27±0.05、0.31 ±0.03、0.33±0.03及0.32 ±0.01)均显著高于LPS组(P＜0.05),PDLC IL-6、IL-1β及TNF-α的分泌量均显著低于LPS组(P＜0.05).结论 GBE对LPS作用下的人PDLC有保护作用.
目的 觀察銀杏葉提取物(Ginkgo biloba extract,GBE)對脂多糖(lipopolysaccharide,LPS)作用下人牙週膜細胞(periodental ligament cell,PDLC)增殖及分泌白細胞介素(interleukin,IL)6、IL-1β及腫瘤壞死因子α(tumor necrosis factor-α,TNF-α)的影響,為GBE在牙週病防治中的應用提供依據.方法 選取2010年5至7月在遵義醫學院附屬口腔醫院口腔頜麵外科門診因正畸拔除的10例11 ～15歲患者的20顆前磨牙,採用改良組織塊培養法體外原代培養人PDLC,實驗共分5組:①陰性對照組,含10 ml/L胎牛血清的達爾伯剋改良伊格爾培養基(Dulbecco's modified Eagle's medium,DMEM)培養液；②LPS組,含10 ml/L胎牛血清的DMEM培養液+100 mg/L LPS；③LPS+0.1 g/L GBE組,含10 ml/L胎牛血清的DMEM培養液+100 mg/L LPS+0.1g/L GBE；④LPS+0.01 g/L GBE組,含10 ml/L胎牛血清的DMEM培養液+100 mg/L LPS +0.01 g/L GBE;⑤LPS+地塞米鬆組,含10 ml/L胎牛血清的DMEM培養液+100 mg/L LPS+2 mg/L地塞米鬆.甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法測定GBE對LPS作用下人PDLC活性的影響,酶聯免疫吸附測定法測定各組IL-6、IL-1β及TNF-α的含量,對所得結果分彆進行單因素方差分析,採用LSD-t檢驗進行組間兩兩比較,檢驗水準為雙側α=0.05.結果 實驗12、24、48及72 h,LPS+0.1 g/L GBE組的吸光度值(分彆為0.30±0.03、0.33±0.02、0.34 ±0.02及0.35 ±0.02)均顯著高于LPS組(分彆為0.20±0.03、0.20±0.02、0.22±0.04及0.24±0.02)(P＜0.05),PDLC IL-6、IL-1 β及TNF-α的分泌量均顯著低于LPS組(P＜0.05)；實驗12、24、48及72 h,LPS+0.01 g/L GBE組吸光度值(分彆為0.27±0.05、0.31 ±0.03、0.33±0.03及0.32 ±0.01)均顯著高于LPS組(P＜0.05),PDLC IL-6、IL-1β及TNF-α的分泌量均顯著低于LPS組(P＜0.05).結論 GBE對LPS作用下的人PDLC有保護作用.
목적 관찰은행협제취물(Ginkgo biloba extract,GBE)대지다당(lipopolysaccharide,LPS)작용하인아주막세포(periodental ligament cell,PDLC)증식급분비백세포개소(interleukin,IL)6、IL-1β급종류배사인자α(tumor necrosis factor-α,TNF-α)적영향,위GBE재아주병방치중적응용제공의거.방법 선취2010년5지7월재준의의학원부속구강의원구강합면외과문진인정기발제적10례11 ～15세환자적20과전마아,채용개량조직괴배양법체외원대배양인PDLC,실험공분5조:①음성대조조,함10 ml/L태우혈청적체이백극개량이격이배양기(Dulbecco's modified Eagle's medium,DMEM)배양액；②LPS조,함10 ml/L태우혈청적DMEM배양액+100 mg/L LPS；③LPS+0.1 g/L GBE조,함10 ml/L태우혈청적DMEM배양액+100 mg/L LPS+0.1g/L GBE；④LPS+0.01 g/L GBE조,함10 ml/L태우혈청적DMEM배양액+100 mg/L LPS +0.01 g/L GBE;⑤LPS+지새미송조,함10 ml/L태우혈청적DMEM배양액+100 mg/L LPS+2 mg/L지새미송.갑기새서기사서(methyl thiazolyl tetrazolium,MTT)법측정GBE대LPS작용하인PDLC활성적영향,매련면역흡부측정법측정각조IL-6、IL-1β급TNF-α적함량,대소득결과분별진행단인소방차분석,채용LSD-t검험진행조간량량비교,검험수준위쌍측α=0.05.결과 실험12、24、48급72 h,LPS+0.1 g/L GBE조적흡광도치(분별위0.30±0.03、0.33±0.02、0.34 ±0.02급0.35 ±0.02)균현저고우LPS조(분별위0.20±0.03、0.20±0.02、0.22±0.04급0.24±0.02)(P＜0.05),PDLC IL-6、IL-1 β급TNF-α적분비량균현저저우LPS조(P＜0.05)；실험12、24、48급72 h,LPS+0.01 g/L GBE조흡광도치(분별위0.27±0.05、0.31 ±0.03、0.33±0.03급0.32 ±0.01)균현저고우LPS조(P＜0.05),PDLC IL-6、IL-1β급TNF-α적분비량균현저저우LPS조(P＜0.05).결론 GBE대LPS작용하적인PDLC유보호작용.
Objective To investigate the effects of Ginkgo biloba extract(GBE) on the proliferation and expression of tumor necrosis factor-a(TNF-α),interleukin(IL)-6,IL-1 β in human periodontal ligament cells (PDLC) treated with lipopolysaccharide (LPS).Methods Human PDLC were cultured with modified tissue culture method.Methyl thiazolyl tetrazolium (MTT) colorimetric assay was used to assess the cell proliferation.Enzyme-linked immunosorbent assay (ELISA) was applied to detect the level of TNF-α,IL-6,IL-1β in the culture supernatant.One-way ANOVA and LSD-t were used to analyze the data.Results The absorbance in LPS +0.1 g/L GBE group was 0.30 ±0.03,0.33 ±0.02,0.34 ±0.02,0.35 ±0.02 at 12,24,48 and 72 h,respectively,higher than that in LPS group (0.20 ± 0.03,0.20 ± 0.02,0.22 ± 0.04,0.24 ± 0.02) (P ＜ 0.05).IL-6,IL-1β and TNF-α in PDLC were lower than that in LPS group (P ＜ 0.05).The absorbance in LPS +0.01 g/L GBE group was 0.27 ±0.05,0.31 ±0.03,0.33 ±0.03,0.32 ±0.01,higher than that in LPS group (P ＜ 0.05).IL-6,IL-lβ and TNF-α in PDLC were lower than LPS group (P ＜ 0.05).Conclusions GBE has the protective effects on human PDLC via inhibiting LPS.
