CAJ | 학술논문

目的建立基于聚吡咯修饰电极的阻抗免疫传感器,以期为实现椅旁牙周细菌检测的快速量化创造条件.方法通过微加工技术制备传感器,传感器微电极表面修饰聚吡咯,聚吡咯表面固定牙龈卟啉单胞菌抗体,通过波特图和阻抗-细菌浓度函数关系,分析传感器的阻抗特性和量化牙龈卟啉单胞菌检测的可行性.结果传感器的最低检测限为107个/L,检测线性范围为107～1012个/L,选择性好,检测时间在1h内.结论该传感器在一定范围内能够用于牙龈卟啉单胞菌的快速量化.
목적 건립기우취필각수식전겁적조항면역전감기,이기위실현의방아주세균검측적쾌속양화창조조건.방법 통과미가공기술제비전감기,전감기미전겁표면수식취필각,취필각표면고정아간계람단포균항체,통과파특도화조항-세균농도함수관계,분석전감기적조항특성화양화아간계람단포균검측적가행성.결과 전감기적최저검측한위107개/L,검측선성범위위107～1012개/L,선택성호,검측시간재1h내.결론 해전감기재일정범위내능구용우아간계람단포균적쾌속양화.
Objective To develop an electrochemical impedance immunosensor based on polypyrrole modified microelectrodes for periodontal bacteria rapid quantification.Methods Mcirofluidic chip with embedded IDAM was prepared by conventional photolithography process.Then polypyrrole structure was deposited on microelectrodes through electropolymerization method.Polypyrrole was biofuncationalized with mouse anti-Porphyromonas gingivalis gingipain K IgG antibody using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide-hydrochloride (EDC) chemistry.The linear relationship between the impedance and bacterial concentration,the impedance characteristic and the feasibility of the developed impedic immunosensor in quantifing Porphyromonas gingivalis was investigated respectively.Results The polypyrrole membrane structure was observed on work electrodes,and the lowest detection limit of the immunosensor was 107 cells/L in pure culture.In the concentration range of 107-1012 cells/L,a linear relationship was found between the normalized impedance change and the logarithmic value of the cell concentration.The total detection time from sampling to measurement was less than 1 h.Conclusions The immunosensor developed in the present study offered some insight into chair-side quantifing periodontal bacteria.