中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
z1期
125-128
,共4页
富组蛋白质类%动力学%姜黄素%茶黄素%矢车菊素
富組蛋白質類%動力學%薑黃素%茶黃素%矢車菊素
부조단백질류%동역학%강황소%다황소%시차국소
Histatins%Kinetics%Curcumin%Theaflavin%Cyanidin
目的 通过实时动态监测茶黄素、姜黄素和矢车菊素吸附于人唾液富组蛋白5表面的动力学过程,探讨牙面着色的形成机制.方法 通过表面等离子体共振(surface plasmon resonance,SPR)芯片表面自组装富组蛋白5单分子膜,监测茶黄素、姜黄素和矢车菊素吸附于该膜表面的过程,根据Langmuir和Freundlich吸附等温线的相关系数(R2)建立3种色素在富组蛋白5表面的吸附模型,并计算Langmuir和Freundlich吸附常数(KL和Kf)、最大吸附量(Mm)、结合和解离速率常数(κa和kd)、结合和解离平衡常数(KA和KD),比较3种色素与富组蛋白5的亲和力差异.使用配对t检验、单因素方差分析和SNK-q检验比较色素与富组蛋白5之间吸附动力学常数的差异,检验水准为双侧α=0.05.结果 Freundlich模型较Langmuir更适于描述色素吸附于富组蛋白5表面的过程.吸附动力学常数分析证实,色素对富组蛋白5表面的亲和力大小为茶黄素[KD=(1.664±0.072)×10-4 mol/L]＞矢车菊素[KD=(1.932 ±0.034)×10-4 mol/L]＞姜黄素[KD=(2.867 ±0.137)×10-4 mol/L] (P ＜0.05).结论 3种外源性色素中茶黄素对唾液富组蛋白5最具亲和力,可导致较严重的牙着色.
目的 通過實時動態鑑測茶黃素、薑黃素和矢車菊素吸附于人唾液富組蛋白5錶麵的動力學過程,探討牙麵著色的形成機製.方法 通過錶麵等離子體共振(surface plasmon resonance,SPR)芯片錶麵自組裝富組蛋白5單分子膜,鑑測茶黃素、薑黃素和矢車菊素吸附于該膜錶麵的過程,根據Langmuir和Freundlich吸附等溫線的相關繫數(R2)建立3種色素在富組蛋白5錶麵的吸附模型,併計算Langmuir和Freundlich吸附常數(KL和Kf)、最大吸附量(Mm)、結閤和解離速率常數(κa和kd)、結閤和解離平衡常數(KA和KD),比較3種色素與富組蛋白5的親和力差異.使用配對t檢驗、單因素方差分析和SNK-q檢驗比較色素與富組蛋白5之間吸附動力學常數的差異,檢驗水準為雙側α=0.05.結果 Freundlich模型較Langmuir更適于描述色素吸附于富組蛋白5錶麵的過程.吸附動力學常數分析證實,色素對富組蛋白5錶麵的親和力大小為茶黃素[KD=(1.664±0.072)×10-4 mol/L]＞矢車菊素[KD=(1.932 ±0.034)×10-4 mol/L]＞薑黃素[KD=(2.867 ±0.137)×10-4 mol/L] (P ＜0.05).結論 3種外源性色素中茶黃素對唾液富組蛋白5最具親和力,可導緻較嚴重的牙著色.
목적 통과실시동태감측다황소、강황소화시차국소흡부우인타액부조단백5표면적동역학과정,탐토아면착색적형성궤제.방법 통과표면등리자체공진(surface plasmon resonance,SPR)심편표면자조장부조단백5단분자막,감측다황소、강황소화시차국소흡부우해막표면적과정,근거Langmuir화Freundlich흡부등온선적상관계수(R2)건립3충색소재부조단백5표면적흡부모형,병계산Langmuir화Freundlich흡부상수(KL화Kf)、최대흡부량(Mm)、결합화해리속솔상수(κa화kd)、결합화해리평형상수(KA화KD),비교3충색소여부조단백5적친화력차이.사용배대t검험、단인소방차분석화SNK-q검험비교색소여부조단백5지간흡부동역학상수적차이,검험수준위쌍측α=0.05.결과 Freundlich모형교Langmuir경괄우묘술색소흡부우부조단백5표면적과정.흡부동역학상수분석증실,색소대부조단백5표면적친화력대소위다황소[KD=(1.664±0.072)×10-4 mol/L]＞시차국소[KD=(1.932 ±0.034)×10-4 mol/L]＞강황소[KD=(2.867 ±0.137)×10-4 mol/L] (P ＜0.05).결론 3충외원성색소중다황소대타액부조단백5최구친화력,가도치교엄중적아착색.
Ohjeetive To monitor the dynamic binding process of three natural phenolic pigments (theaflavin,TF; curcumin,Cur; cyanidin,Cy) on human salivary rich histidine 5 (H5) surface in situ real-time and dynamic by surface plasmon resonance (SPR) technique at the molecular level.Methods In situ dynamic monitoring the binding process of three kinds of pigments to self-assembled monolayer biofilm of human rich histatin 5 on SPR sensor surface in real time.The adsorption models of three kinds of pigments were decided by the correlation coefficient (R2) of Langmuir and Freundlich adsorption isotherm.Langmuir and Freundlich adsorption constants (KL and Kf),maximal mass of adsorption (Mm),association and dissociation rate constants(ka and kd),association and dissociation equilibrium constants(KA and KD) were calculated,respectively.The binding affinity for phenolic pigments to human salivary rich histidine 5 was compared.The data were analyzed using paired samples t-test and one-way ANOVA and SNK-q test.The level of significance was defined as α =0.05.Results The results showed that the adsorption of pigments on H5 surface was better described by Freundlich than by Langmuir.The affinities of the pigments to H5 were TF[KD =(1.664 ±0.072) x 10-4 mol/L] ＞ Cy[KD =(1.932 ±0.034) x 10-4 mol/L] ＞ Cur[KD =(2.867 ± 0.137) x 10-4 mol/L] (P ＜ 0.05),which were evidenced by the dynamic constants.Conclusions The affinity of theaflavin to salivary rich histidine 5 is the highest among the three kinds of pigments,which means that theaflavin may result in more serious tooth stain in the oral cavity.
