CAJ | 학술논문

目的评估HIV+牙周炎患者的CD4+ T淋巴细胞计数(简称CD4计数)和龈沟液中前列腺素E2(prostaglandin E2,PGE2)与牙周临床指标的相关性,以期为临床提供参考.方法选择符合纳入标准的20例HIV+牙周炎患者,计数其血液CD4+T淋巴细胞.按CD4计数分组:A组(CD4计数＜ 200个/mm3,共计5例30颗牙)、B组(CD4计数为200～500个/mm3,共计13例78颗牙)、C组(CD4计数＞500个/mm3,共计2例12颗牙),对受试者共计120颗指数牙进行牙周检查:菌斑指数(plaque index,PLI)、出血指数(bleeding index,BI)、附着水平(attachment level,AL)、牙周探诊深度(probing depth,PD),用放射免疫法检测龈沟液中PGE2.组间牙周临床指标和PGE2的比较采用Mann-Whitney秩和检验,CD4计数和牙周临床指标的相关性采用偏相关分析,PGE2与牙周临床指标的关系采用Spearman相关分析.结果 B组的BI值、PGE2质量浓度、PGE2总量分别为3.00(2.00)、90.75(30.60) μg/L及447.58(243.08) pg,均显著高于A组[分别为2.00(1.25)、79.75 (30.50) μg/L、339.52(200.97) pg]和C组[分别为2.00(1.00)、73.38(14.83) μg/L、299.18(108.33) pg](P＜0.0167),而A组和C组的各项牙周临床指标差异及三组两两之间的PD和AL差异均无统计学意义(P＞0.0167).A组和B组的CD4计数与BI有相关性,偏相关系数分别为0.657(P ＜0.05)、-0.369(P＜0.05);C组的CD4计数与BI无相关性(P＞0.05).PGE2质量浓度与探诊深度、附着丧失呈负相关(P＜0.05),PGE2总量与各项牙周临床指标呈正相关(P＜0.05).结论 HIV+牙周炎患者的牙周炎症程度与CD4计数有关,其龈沟液中PGE2水平与牙周临床指标有关.
목적 평고HIV+아주염환자적CD4+ T림파세포계수(간칭CD4계수)화간구액중전렬선소E2(prostaglandin E2,PGE2)여아주림상지표적상관성,이기위림상제공삼고.방법 선택부합납입표준적20례HIV+아주염환자,계수기혈액CD4+T림파세포.안CD4계수분조:A조(CD4계수＜ 200개/mm3,공계5례30과아)、B조(CD4계수위200～500개/mm3,공계13례78과아)、C조(CD4계수＞500개/mm3,공계2례12과아),대수시자공계120과지수아진행아주검사:균반지수(plaque index,PLI)、출혈지수(bleeding index,BI)、부착수평(attachment level,AL)、아주탐진심도(probing depth,PD),용방사면역법검측간구액중PGE2.조간아주림상지표화PGE2적비교채용Mann-Whitney질화검험,CD4계수화아주림상지표적상관성채용편상관분석,PGE2여아주림상지표적관계채용Spearman상관분석.결과 B조적BI치、PGE2질량농도、PGE2총량분별위3.00(2.00)、90.75(30.60) μg/L급447.58(243.08) pg,균현저고우A조[분별위2.00(1.25)、79.75 (30.50) μg/L、339.52(200.97) pg]화C조[분별위2.00(1.00)、73.38(14.83) μg/L、299.18(108.33) pg](P＜0.0167),이A조화C조적각항아주림상지표차이급삼조량량지간적PD화AL차이균무통계학의의(P＞0.0167).A조화B조적CD4계수여BI유상관성,편상관계수분별위0.657(P ＜0.05)、-0.369(P＜0.05);C조적CD4계수여BI무상관성(P＞0.05).PGE2질량농도여탐진심도、부착상실정부상관(P＜0.05),PGE2총량여각항아주림상지표정정상관(P＜0.05).결론 HIV+아주염환자적아주염증정도여CD4계수유관,기간구액중PGE2수평여아주림상지표유관.
Objective To investigate the correlation of CD4+ T lymphocyte count and prostaglandin E2 (PGE2) in gingival crevicular fluid(GCF) with periodontal status in HIV-positive patients with periodontitis.Methods Twenty subjects were selected according to inclusion criteria.The plasmatic CD4+ T lymphocytes were counted.All the individuals were divided into three groups,group A (CD4+ T lymphocyte count ＜200 cell/mm3),group B (200 cell/mm3 ≤CD4+ T lymphocyte count≤500 cell/mm3) and group C (CD4+ T lymphocyte count ＞ 500 cell/mm3).Periodontal indexes,including plaque index (PLI),bleeding index(BI),attachment level(AL) and probing depth(PD) were recorded.GCF samples were taken from 120 index teeth by means of sterile paper strips.GCF PGE2 levels were determined by radioimmunoassays.MannWhitney was used to compare the periodontal indexes and PGE2 levels among the three groups.Partial correlations and Spearman correlations were applied to analyze the correlation of CD4+ T lymphocytes count and PGE2 in gingival crevicular fluid with periodontal status.Results BI value,PGE2 concentration and total PGE2 were 3.00 (2.00),90.75 (30.60) μg,/L,447.58 (243.08) pg in group B,which were higher than those in group A[2.00(1.25),79.75(30.50) μg/L and 339.52(200.97) pg respectively] and group C [2.00(1.00),73.38 (14.83) μg/L and 299.18 (108.33) pg respectively] (P ＜ 0.0167).But the differences of PD and AL among the three groups were not significantly different (P ＞ 0.0167).The correlations were observed between CD4+ T lymphocyte count and BI for the subpopulations with CD4+ T lymphocyte count ＜ 200 cells/mm3 (r =0.657,P ＜ 0.05) and between 200-500 cells/mm3 (r =-0.369,P ＜ O.05).PGE2 concentration was negatively correlated with BI,PD and AL(P ＜ 0.05),and total PGE2 was positively correlated with PD and AL (P ＜ 0.05).Conclusions There was an association between the periodontal status and CD4+ T lymphocyte count in HIV + patients.GCF PGE2 level was related to periodontal parameters including BI,PD and AL.