中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
2期
84-88
,共5页
牙种植体%组织工程%再生%牙周膜细胞
牙種植體%組織工程%再生%牙週膜細胞
아충식체%조직공정%재생%아주막세포
Dental implants%Tissue engineering%Regeneration%Periodontal ligament cell
目的 应用牙周膜细胞(periodontal ligament cells,PDLC)膜片、骨髓基质细胞(bone marrow stromal cells,BMSC)膜片与自体牙根体外构建牙生物性种植体,研究构建具有新生牙周组织支持的生物性牙根的可行性.方法 拔除2只比格犬下颌两侧双尖牙制作缺牙区,将比格犬自体牙根预备成近似圆柱状.培养犬第3代PDLC和BMSC成细胞膜片,体外将PDLC和BMSC膜片单独或共同包裹于牙根表面构建自体牙根种植体,将种植体分为4组:①双膜片组(每只犬2颗),将PDLC、BMSC先后包裹于同一牙根表面;②PDLC膜片组(每只犬2颗),将PDLC单膜片包裹于牙根表面;③BMSC膜片组(每只犬2颗),将BMSC单膜片包裹于牙根表面;④无膜片组(空白对照组)(每只犬1颗),无膜片牙根.制作缺牙区手术8周后,将种植体植入自体缺牙区牙槽嵴,12周时取材制作石蜡切片行HE和Masson三色染色,观察各组切片的组织学结果.结果 PDLC膜片组种植体根面可见包括牙骨质、牙周膜及牙槽骨的牙周组织样结构,其中可见类似牙周穿通纤维样组织垂直插入根面及骨面;双膜片组种植体和BMSC膜片组种植体根面多为骨性结合;空白对照组亦未见牙周组织样结构形成.结论 PDLC膜片能促进牙生物性种植体的牙周再生,可部分再生出包括牙骨质、牙周膜和牙槽骨的复杂牙周组织.
目的 應用牙週膜細胞(periodontal ligament cells,PDLC)膜片、骨髓基質細胞(bone marrow stromal cells,BMSC)膜片與自體牙根體外構建牙生物性種植體,研究構建具有新生牙週組織支持的生物性牙根的可行性.方法 拔除2隻比格犬下頜兩側雙尖牙製作缺牙區,將比格犬自體牙根預備成近似圓柱狀.培養犬第3代PDLC和BMSC成細胞膜片,體外將PDLC和BMSC膜片單獨或共同包裹于牙根錶麵構建自體牙根種植體,將種植體分為4組:①雙膜片組(每隻犬2顆),將PDLC、BMSC先後包裹于同一牙根錶麵;②PDLC膜片組(每隻犬2顆),將PDLC單膜片包裹于牙根錶麵;③BMSC膜片組(每隻犬2顆),將BMSC單膜片包裹于牙根錶麵;④無膜片組(空白對照組)(每隻犬1顆),無膜片牙根.製作缺牙區手術8週後,將種植體植入自體缺牙區牙槽嵴,12週時取材製作石蠟切片行HE和Masson三色染色,觀察各組切片的組織學結果.結果 PDLC膜片組種植體根麵可見包括牙骨質、牙週膜及牙槽骨的牙週組織樣結構,其中可見類似牙週穿通纖維樣組織垂直插入根麵及骨麵;雙膜片組種植體和BMSC膜片組種植體根麵多為骨性結閤;空白對照組亦未見牙週組織樣結構形成.結論 PDLC膜片能促進牙生物性種植體的牙週再生,可部分再生齣包括牙骨質、牙週膜和牙槽骨的複雜牙週組織.
목적 응용아주막세포(periodontal ligament cells,PDLC)막편、골수기질세포(bone marrow stromal cells,BMSC)막편여자체아근체외구건아생물성충식체,연구구건구유신생아주조직지지적생물성아근적가행성.방법 발제2지비격견하합량측쌍첨아제작결아구,장비격견자체아근예비성근사원주상.배양견제3대PDLC화BMSC성세포막편,체외장PDLC화BMSC막편단독혹공동포과우아근표면구건자체아근충식체,장충식체분위4조:①쌍막편조(매지견2과),장PDLC、BMSC선후포과우동일아근표면;②PDLC막편조(매지견2과),장PDLC단막편포과우아근표면;③BMSC막편조(매지견2과),장BMSC단막편포과우아근표면;④무막편조(공백대조조)(매지견1과),무막편아근.제작결아구수술8주후,장충식체식입자체결아구아조척,12주시취재제작석사절편행HE화Masson삼색염색,관찰각조절편적조직학결과.결과 PDLC막편조충식체근면가견포괄아골질、아주막급아조골적아주조직양결구,기중가견유사아주천통섬유양조직수직삽입근면급골면;쌍막편조충식체화BMSC막편조충식체근면다위골성결합;공백대조조역미견아주조직양결구형성.결론 PDLC막편능촉진아생물성충식체적아주재생,가부분재생출포괄아골질、아주막화아조골적복잡아주조직.
Objective To fabricate the bio-implant supported by regenerated periodontal tissue utilizing periodontal ligament cells(PDLC)-bone marrow stromal cells(BMSC) sheet and natural root.Methods Premolars of 2 beagle dogs were extracted to prepare the implanted area.Autologous tooth roots were carved into cylinders.PDLC and BMSC separated from beagle dogs were cultured into cell sheets in medium.Tooth roots were wrapped by one type of cell sheets or both to fabricate bio-implant and divided into four groups,tooth roots were wrapped by PDLC sheets and BMSC sheets successively (2 samples each dog),tooth roots were wrapped by PDLC sheets alone (2 samples each dog),tooth roots were wrapped by BMSC sheet alone (2 samples each dog),tooth roots without cell sheet (1 sample each dog).The implants were implanted into the mandibles.The mandibles were dissected 12 weeks later,sliced and stained by HE and Masson dyes for histological examination.Results In the PDLC cell sheet/root implants,histological examination revealed that new periodontal-like tissue including cementum,periodontium and alveolar bone was regenerated.In the BMSC implants,tooth ankylosis was observed.Conclusions PDLC sheet and natural root can be used to fabricate bio-implant.PDLC sheet could promote periodontal regeneration.
