中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
3期
145-150
,共6页
周彦玢%刘大力%李云鹏%王依玮%束蓉
週彥玢%劉大力%李雲鵬%王依瑋%束蓉
주언분%류대력%리운붕%왕의위%속용
紫单胞菌,龈%细菌荚膜%临床菌株%表面性质
紫單胞菌,齦%細菌莢膜%臨床菌株%錶麵性質
자단포균,간%세균협막%림상균주%표면성질
Porphyromonas gingivalis%Bacterial capsules%Clinical isolates%Surface properties
目的 分析牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)临床菌株的多种表面性质,初步探讨Pg菌株荚膜与其表面性质的关系.方法 利用透射电镜观测5株临床菌株(SJD2、SJD12、SJD4、SJD5和SJD11)和2株模式菌株(W83、ATCC33277)的荚膜结构和厚度,采用微生物烃类黏附实 验检测受试菌株的表面亲疏水性质,并使用自凝集实验观察受试菌株浮游生长的能力.采用生物膜半定量检测和激光扫描共聚焦显微镜检测各菌株形成生物膜的能力.应用Student-Newman Kewls检验对结果数据进行组间两两比较,以双侧P＜0.05为差异有统计学意义.结果 Pg菌株的荚膜厚度各异,高毒模式菌株W83的荚膜厚度[(25.11 ±1.18) nm]显著大于低毒模式菌株ATCC33277[(14.87±1.01) nm] (P ＜0.05).临床菌株的荚膜厚度由厚至薄依次为:SJD4、SJD11、SJD5、SJD2、SJD12.荚膜较厚的W83、SJD4、SJD11菌株疏水率低,细菌不易自凝集,呈浮游生长;荚膜较薄的ATCC33277、SJD5、SJD2、SJD12菌株疏水率较W83显著上升,且差异有统计学意义(P＜0.05),自凝集现象明显.生物膜半定量检测实验中,除SJD2和SJD4的生物膜染色后具有相同水平的吸光度之外,其余菌株吸光度差异均有统计学意义(P＜0.05),各菌株生物膜中的细菌量并不与荚膜厚度的差异相吻合.各菌株生物膜厚度从(14.74 ±4.99)至(24.13 ±5.45) μm不等,差异均无统计学意义(p＞0.05).激光扫描共聚焦显微镜观察可见W83、SJD11生物膜内荧光信号较弱,生物膜密度较低,其他菌株可生成较致密且荧光信号较强的生物膜结构,存在空隙或孔道结构.结论 Pg菌株表面性质的多样性与荚膜厚度存在一定关联.
目的 分析牙齦卟啉單胞菌(Porphyromonas gingivalis,Pg)臨床菌株的多種錶麵性質,初步探討Pg菌株莢膜與其錶麵性質的關繫.方法 利用透射電鏡觀測5株臨床菌株(SJD2、SJD12、SJD4、SJD5和SJD11)和2株模式菌株(W83、ATCC33277)的莢膜結構和厚度,採用微生物烴類黏附實 驗檢測受試菌株的錶麵親疏水性質,併使用自凝集實驗觀察受試菌株浮遊生長的能力.採用生物膜半定量檢測和激光掃描共聚焦顯微鏡檢測各菌株形成生物膜的能力.應用Student-Newman Kewls檢驗對結果數據進行組間兩兩比較,以雙側P＜0.05為差異有統計學意義.結果 Pg菌株的莢膜厚度各異,高毒模式菌株W83的莢膜厚度[(25.11 ±1.18) nm]顯著大于低毒模式菌株ATCC33277[(14.87±1.01) nm] (P ＜0.05).臨床菌株的莢膜厚度由厚至薄依次為:SJD4、SJD11、SJD5、SJD2、SJD12.莢膜較厚的W83、SJD4、SJD11菌株疏水率低,細菌不易自凝集,呈浮遊生長;莢膜較薄的ATCC33277、SJD5、SJD2、SJD12菌株疏水率較W83顯著上升,且差異有統計學意義(P＜0.05),自凝集現象明顯.生物膜半定量檢測實驗中,除SJD2和SJD4的生物膜染色後具有相同水平的吸光度之外,其餘菌株吸光度差異均有統計學意義(P＜0.05),各菌株生物膜中的細菌量併不與莢膜厚度的差異相吻閤.各菌株生物膜厚度從(14.74 ±4.99)至(24.13 ±5.45) μm不等,差異均無統計學意義(p＞0.05).激光掃描共聚焦顯微鏡觀察可見W83、SJD11生物膜內熒光信號較弱,生物膜密度較低,其他菌株可生成較緻密且熒光信號較彊的生物膜結構,存在空隙或孔道結構.結論 Pg菌株錶麵性質的多樣性與莢膜厚度存在一定關聯.
목적 분석아간계람단포균(Porphyromonas gingivalis,Pg)림상균주적다충표면성질,초보탐토Pg균주협막여기표면성질적관계.방법 이용투사전경관측5주림상균주(SJD2、SJD12、SJD4、SJD5화SJD11)화2주모식균주(W83、ATCC33277)적협막결구화후도,채용미생물경류점부실 험검측수시균주적표면친소수성질,병사용자응집실험관찰수시균주부유생장적능력.채용생물막반정량검측화격광소묘공취초현미경검측각균주형성생물막적능력.응용Student-Newman Kewls검험대결과수거진행조간량량비교,이쌍측P＜0.05위차이유통계학의의.결과 Pg균주적협막후도각이,고독모식균주W83적협막후도[(25.11 ±1.18) nm]현저대우저독모식균주ATCC33277[(14.87±1.01) nm] (P ＜0.05).림상균주적협막후도유후지박의차위:SJD4、SJD11、SJD5、SJD2、SJD12.협막교후적W83、SJD4、SJD11균주소수솔저,세균불역자응집,정부유생장;협막교박적ATCC33277、SJD5、SJD2、SJD12균주소수솔교W83현저상승,차차이유통계학의의(P＜0.05),자응집현상명현.생물막반정량검측실험중,제SJD2화SJD4적생물막염색후구유상동수평적흡광도지외,기여균주흡광도차이균유통계학의의(P＜0.05),각균주생물막중적세균량병불여협막후도적차이상문합.각균주생물막후도종(14.74 ±4.99)지(24.13 ±5.45) μm불등,차이균무통계학의의(p＞0.05).격광소묘공취초현미경관찰가견W83、SJD11생물막내형광신호교약,생물막밀도교저,기타균주가생성교치밀차형광신호교강적생물막결구,존재공극혹공도결구.결론 Pg균주표면성질적다양성여협막후도존재일정관련.
Objective To analyze the capsule related surface properties of Porphyromonas gingivalis (Pg) isolates.Methods The transmission electron microscopy (TEM) was used to observe the capsule structure and the capsule thickness of 2 type of strains and 5 clinical isolates.Microbial adhesion to hydrocarbons(MATH) assay was used to qualitatively assess the hydrophobicity of each strain,and the capacities of these strains were investigated by autoaggregation assay.Ninety-six well biofilm assay and confocal laser scanning microscopy (CLSM) were applied to quantify and observe the biofilm produced by each strain.Results TEM showed the variety of capsule thickness of these strains.Virulent type strain W83 possessed thicker capsular structure than less-virulent type strain ATCC33277.The SJD4 possessed thicker capsule than other clinical isolates,followed by SJD11,SJD5,SJD2,and SJD12.Strains W83,SJD4,SJD11,with thicker capsule,were much more hydrophilic with lower MATH percentage,in accordance with a slow autoaggregation in incubation during a period of 240 min.Compared with W83,the hydrophobicity of strains ATCC33277,SJD5,SJD2,and SJD12,with thinner capsule,showed increased MATH percentage and autoaggregations.All clinical strains developed biofilm with different absorbance compared with type strains.The CLSM observation showed biofilm thickness of each strain,ranged from (14.74 ± 4.99) to (24.13-± 5.45) μm.Strain W83 and SJD11 showed notable poor biofilm formation,while others developed dense and mature biofilm.Conclusions There was a certain degree of linkage between the Pg capsule thickness and surface properties diversity.
