CAJ | 학술논문

目的观察3岁儿童从无龋到患龋过程中龋易感因素的变化,以期为低龄儿童龋齿风险评估提供相关依据.方法选择144名34 ～38个月龄无龋儿童观察1年.在观察基线、6个月及12个月时分别采集口腔龈上集合菌斑和相关问卷调查;根据各观察期末不同患龋状况,将研究对象分成无龋组(12个月保持无龋)、6个月患龋组(6个月时有龋或充填体)和12个月患龋组(6个月时无龋、12个月时有龋或充填体).应用实时荧光定量PCR比较不同组间变形链球菌(Streptococcus mutans,Sm)和远缘链球菌(Streptococcus sobrinus,Ss)数量的差异.结果 130名儿童完成了12个月的复查,失访率为9.7％(14/144).在12个月观察期末,无龋组为58人,6个月患龋组为44人,12个月患龋组为28人.在基线、6个月和12个月时3组儿童的口腔卫生和饮食习惯自身前后对比差异均无统计学意义(P＞0.05).基线时无龋组进甜食和睡前进甜食或甜饮料的频率均显著低于6个月患龋组和12个月患龋组(P ＜0.001).基线时6个月患龋组Sm和Ss的数量[Sm拷贝数:(6.33±2.21)×103,Ss拷贝数:(1.99±0.45)×103]均显著高于无龋组[Sm拷贝数:(5.81 ±2.43)×103,Ss拷贝数:(1.34±0.53)×103] (P ＜0.05);6个月复查时,12个月患龋组Sm和Ss的数量[Sm拷贝数:(6.81 ±2.32)×103,Ss拷贝数:(1.97±1.43)×103]均显著高于无龋组[Sm拷贝数:(6.09±2.31)×103,Ss拷贝数:(1.34±0.55)×103] (P ＜0.05).结论 3岁儿童在临床检出龋齿前6个月时Sm和Ss数量已显著升高;高频率摄取甜食及睡前进甜食或甜饮料是儿童未来患龋的重要易感因素.
목적 관찰3세인동종무우도환우과정중우역감인소적변화,이기위저령인동우치풍험평고제공상관의거.방법 선택144명34 ～38개월령무우인동관찰1년.재관찰기선、6개월급12개월시분별채집구강간상집합균반화상관문권조사;근거각관찰기말불동환우상황,장연구대상분성무우조(12개월보지무우)、6개월환우조(6개월시유우혹충전체)화12개월환우조(6개월시무우、12개월시유우혹충전체).응용실시형광정량PCR비교불동조간변형련구균(Streptococcus mutans,Sm)화원연련구균(Streptococcus sobrinus,Ss)수량적차이.결과 130명인동완성료12개월적복사,실방솔위9.7％(14/144).재12개월관찰기말,무우조위58인,6개월환우조위44인,12개월환우조위28인.재기선、6개월화12개월시3조인동적구강위생화음식습관자신전후대비차이균무통계학의의(P＞0.05).기선시무우조진첨식화수전진첨식혹첨음료적빈솔균현저저우6개월환우조화12개월환우조(P ＜0.001).기선시6개월환우조Sm화Ss적수량[Sm고패수:(6.33±2.21)×103,Ss고패수:(1.99±0.45)×103]균현저고우무우조[Sm고패수:(5.81 ±2.43)×103,Ss고패수:(1.34±0.53)×103] (P ＜0.05);6개월복사시,12개월환우조Sm화Ss적수량[Sm고패수:(6.81 ±2.32)×103,Ss고패수:(1.97±1.43)×103]균현저고우무우조[Sm고패수:(6.09±2.31)×103,Ss고패수:(1.34±0.55)×103] (P ＜0.05).결론 3세인동재림상검출우치전6개월시Sm화Ss수량이현저승고;고빈솔섭취첨식급수전진첨식혹첨음료시인동미래환우적중요역감인소.
Objective To investigate the changes of caries susceptibility factors during the transition from caries-free to caries in three-year-old children.Methods The study included 144 caries-free children aged 34-38 months.All participants underwent clinical examination and full-mouth supragingival plaque collection.Survey of children's oral health behaviors involving feeding habits and oral health care was also taken from children' s parents of all participants.The children were reexamined 6 and 12 months later.According to the potential changes of caries status in twelve months follow up,all the participants were divided into three groups:caries-free group (caries free children in twelve months follow up),caries at 6-month group(children who were found with caries or cavities fillings at six months review) and caries at 12-month group(children who were caries free at six months but found with caries or cavities fillings at twelve months).Streptococcus mutans (Sm)and Streptococcus sobrinus (Ss) loads were compared among the three groups by real time fluorescence quantitative polymerase chain reaction (FQ-PCR).Results One hundred and thirty children completed twelve months review,the dropout rate was 9.7％ (14/144).At the end of twelve months follow up,the number of children in caries-free group,caries at 6-month group and caries at 12-month group was 58,44 and 28 respectively.The changes of oral behavioral habits in caries-free group,caries at 6-month group and caries at 12-month group during twelve months follow up were not significantly different(P ＞0.05).At baseline,the frequency of sweets consumption and eating sweets before sleeping in caries-free group was significantly lower than that in caries at 6-month group and caries at 12-month group (P ＜ 0.001).At baseline,the quantity of Sm and Ss in caries at 6-month group [copy number of Sm:(6.33 ± 2.21) × 103,copy number of Ss:(1.99 ±0.45) × 103] was significantly higher than that in the caries-free group [copy number of Sm:(5.81 ± 2.43) × 103,copy number of Ss:(1.34 ± 0.53) × 103] (P ＜ 0.05).There was no significant difference in the quantity of Sm and Ss in the caries-free group and caries at 12-month group(P ＞ 0.05).At six months,the quantity of Sm and Ss in caries at 12-month group was significantly higher than that in the caries-free group although both groups of children were caries-free at six months (P ＜ 0.05).Conclusions The quantity of Sm and Ss increased six months before the caries onset in three-year-old children.High frequency sweets consumption and eating sweets before sleeping were important susceptibility factors for caries onset in the future.