中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
4期
216-219
,共4页
朱德星%刘汾%戴芳%罗雪晴%洪宝枝
硃德星%劉汾%戴芳%囉雪晴%洪寶枝
주덕성%류분%대방%라설청%홍보지
慢性牙周炎%T淋巴细胞%程序性死亡分子1%程序性死亡分子配体1
慢性牙週炎%T淋巴細胞%程序性死亡分子1%程序性死亡分子配體1
만성아주염%T림파세포%정서성사망분자1%정서성사망분자배체1
Chronic periodontitis%T-lymphocytes%Programmed death-1%Programmed death ligand-1
目的 探讨慢性牙周炎患者外周血淋巴细胞程序性死亡分子1(programmed death-1,PD-1)及其主要配体程序性死亡分子配体1(programmed death ligand-1,PD-L1)的表达及其意义,以阐明其在慢性牙周炎发生和发展中的作用.方法 采用流式细胞术检测30例慢性牙周炎患者(牙周炎组)、25慢性牙龈炎患者(牙龈炎组)及18例牙周健康对照者(健康对照组)外周血CD4+和CD8+T淋巴细胞表面PD-1及PD-L1的表达,并检测16例慢性牙周炎患者进行牙周基础治疗6周后外周血CD4+和CD8+T淋巴细胞PD-1及PD-L1的表达.两组间比较采用t检验,多组间比较采用方差分析,多个样本间的两两比较采用LSD-t检验或x2检验.结果 牙周炎组外周血CD4+、CD8+T淋巴细胞表面PD-1的阳性表达率[(16.7±5.5)％、(20.8±5.1)％]及牙龈炎组二者的表达率[(14.2±6.1)％、(14.5±4.3)％]均显著高于健康对照组[分别为(9.5±2.1)％、(8.1±1.9)％](P值均＜0.05);牙周炎组外周血CD4+、CD8+T淋巴细胞表面PD-L1的阳性表达率[(24.2±7.1)％、(15.3±6.8)％]及牙龈炎组二者的表达率[(12.4±6.0)％、(11.2±5.5)％]均显著高于健康对照组[(4.7±1.2)％和(3.2±2.3)％](P值均＜0.05).牙周基础治疗6周后,16例慢性牙周炎患者CD4+和CD8+T淋巴细胞PD-1、PD-L1的阳性表达率均显著降低,与治疗前相比差异均有统计学意义(P值均＜0.05).结论 慢性牙周炎患者外周血CD4+和CD8+T淋巴细胞PD-1及PD-L1表达水平显著升高,与牙周炎症水平密切相关,牙周基础治疗可以下调慢性牙周炎患者外周血CD4+和CD8+T细胞表面PD-1及PD-L1的表达.
目的 探討慢性牙週炎患者外週血淋巴細胞程序性死亡分子1(programmed death-1,PD-1)及其主要配體程序性死亡分子配體1(programmed death ligand-1,PD-L1)的錶達及其意義,以闡明其在慢性牙週炎髮生和髮展中的作用.方法 採用流式細胞術檢測30例慢性牙週炎患者(牙週炎組)、25慢性牙齦炎患者(牙齦炎組)及18例牙週健康對照者(健康對照組)外週血CD4+和CD8+T淋巴細胞錶麵PD-1及PD-L1的錶達,併檢測16例慢性牙週炎患者進行牙週基礎治療6週後外週血CD4+和CD8+T淋巴細胞PD-1及PD-L1的錶達.兩組間比較採用t檢驗,多組間比較採用方差分析,多箇樣本間的兩兩比較採用LSD-t檢驗或x2檢驗.結果 牙週炎組外週血CD4+、CD8+T淋巴細胞錶麵PD-1的暘性錶達率[(16.7±5.5)％、(20.8±5.1)％]及牙齦炎組二者的錶達率[(14.2±6.1)％、(14.5±4.3)％]均顯著高于健康對照組[分彆為(9.5±2.1)％、(8.1±1.9)％](P值均＜0.05);牙週炎組外週血CD4+、CD8+T淋巴細胞錶麵PD-L1的暘性錶達率[(24.2±7.1)％、(15.3±6.8)％]及牙齦炎組二者的錶達率[(12.4±6.0)％、(11.2±5.5)％]均顯著高于健康對照組[(4.7±1.2)％和(3.2±2.3)％](P值均＜0.05).牙週基礎治療6週後,16例慢性牙週炎患者CD4+和CD8+T淋巴細胞PD-1、PD-L1的暘性錶達率均顯著降低,與治療前相比差異均有統計學意義(P值均＜0.05).結論 慢性牙週炎患者外週血CD4+和CD8+T淋巴細胞PD-1及PD-L1錶達水平顯著升高,與牙週炎癥水平密切相關,牙週基礎治療可以下調慢性牙週炎患者外週血CD4+和CD8+T細胞錶麵PD-1及PD-L1的錶達.
목적 탐토만성아주염환자외주혈림파세포정서성사망분자1(programmed death-1,PD-1)급기주요배체정서성사망분자배체1(programmed death ligand-1,PD-L1)적표체급기의의,이천명기재만성아주염발생화발전중적작용.방법 채용류식세포술검측30례만성아주염환자(아주염조)、25만성아간염환자(아간염조)급18례아주건강대조자(건강대조조)외주혈CD4+화CD8+T림파세포표면PD-1급PD-L1적표체,병검측16례만성아주염환자진행아주기출치료6주후외주혈CD4+화CD8+T림파세포PD-1급PD-L1적표체.량조간비교채용t검험,다조간비교채용방차분석,다개양본간적량량비교채용LSD-t검험혹x2검험.결과 아주염조외주혈CD4+、CD8+T림파세포표면PD-1적양성표체솔[(16.7±5.5)％、(20.8±5.1)％]급아간염조이자적표체솔[(14.2±6.1)％、(14.5±4.3)％]균현저고우건강대조조[분별위(9.5±2.1)％、(8.1±1.9)％](P치균＜0.05);아주염조외주혈CD4+、CD8+T림파세포표면PD-L1적양성표체솔[(24.2±7.1)％、(15.3±6.8)％]급아간염조이자적표체솔[(12.4±6.0)％、(11.2±5.5)％]균현저고우건강대조조[(4.7±1.2)％화(3.2±2.3)％](P치균＜0.05).아주기출치료6주후,16례만성아주염환자CD4+화CD8+T림파세포PD-1、PD-L1적양성표체솔균현저강저,여치료전상비차이균유통계학의의(P치균＜0.05).결론 만성아주염환자외주혈CD4+화CD8+T림파세포PD-1급PD-L1표체수평현저승고,여아주염증수평밀절상관,아주기출치료가이하조만성아주염환자외주혈CD4+화CD8+T세포표면PD-1급PD-L1적표체.
Objective To investigate the expression of programmed death-1(PD-1) and programmed death ligand-1 (PD-L1) in peripheral T-lymphocytes from patients with chronic periodontitis and its significance and to clarify its role in the development of chronic periodontitis.Methods A total of 73 subjects were included in the study and divided into three groups,chronic periodontitis (30 cases),chronic gingivitis(25 cases) and 18 healthy controls.The peripheral blood was collected and PD-1/PD-L1 expression in the surface of CD4+ T lymphocytes and CD8+ T lymphocytes was examined by flow cytometry.Blood samples from 16 chronic periodontitis patients were collected at week 0 and 6 after initial therapy for 6 weeks and PD-1 and PD-L1 expression in the surface of CD4+ and CD8+ T lymphocytes was also determined by flow cytometry.The data were statistically analyzed.Results The percentage of PD-1 expression in CD4+ and CD8+ T lymphocytes of chronic periodontitis group [(16.7 ± 5.5) ％,(20.8 ± 5.1) ％] and chronic gingivitis group [(14.2 ± 6.1) ％,(14.5 ± 4.3) ％] were higher than that of healthy controls [(9.5 ± 2.1) ％,(8.1 ± 1.9) ％] (P ＜ 0.05).The percentage of PD-L1 expression in CD4+ and CD8+ T lymphocytes of chronic periodontitis group [(24.2 ± 7.1) ％,(15.3 ± 6.8) ％] and chronic gingivitis group [(12.4 ±6.0) ％,(11.2 ± 5.5) ％] were higher than that of healthy controls [(4.7 ± 1.2) ％,(3.2 ± 2.3) ％] (P ＜ 0.05).The percentage of PD-1/PD-L1 expression in CD4+ T lymphocytes and CD8+ T lymphocytes of the chronic periodontitis group were significantly decreased after initial therapy(P ＜ 0.05).Conclusions The expression of PD-1 and PD-L1 in peripheral CD4+ T lymphocytes and CD8+ T lymphocyte of chronic periodontitis patients was up-regulated and was associated with periodontal condition.The initial therapy reduced the expression of PD-1 and PD-L1.
