CAJ | 학술논문

目的探讨多聚磷酸钠(sodium tripolyphosphate,STTP)与聚丙烯酸对单层重组Ⅰ型胶原模型的仿生矿化诱导作用,为STTP与聚丙烯酸在牙本质和骨胶原矿化诱导的扩展应用提供依据.方法采用牛表皮Ⅰ型胶原在圆形盖玻片与透射电镜镍网表面建立单层重组胶原模型.联合应用组样本经含STTP与聚丙烯酸的模拟体液-硅酸盐水门汀树脂矿化系统诱导矿化.在矿化诱导第12、24、48和72h取出联合应用组样本(每个时间点样本量为18),用扫描电镜和透射电镜定性分析胶原微观形态学变化,并与矿化诱导72h、不含STTP和聚丙烯酸的空白组(样本量为18),以及仅含STTP或聚丙烯酸的对照组(STTP组、聚丙烯酸组,样本量均为18)进行比较.结果空白组和2个对照组均未见胶原纤维内矿化现象.联合应用组在STTP与聚丙烯酸的协同诱导下矿化12 ～24 h后,重组胶原纤维表面可见纳米级球形电子致密物沉积;随着矿化时间延长,纤维表面无定形电子致密物逐渐消失,代之以胶原纤维内呈高度迭序排列的磷灰石纳米微晶,使纤维呈现典型的周期性横纹结构.结论在模拟体液-硅酸盐水门汀树脂存在的情况下,联合应用STTP和聚丙烯酸能成功诱导磷灰石纳米微晶在不含非胶原蛋白的重组胶原内有序沉积,实现胶原纤维内矿化.
목적 탐토다취린산납(sodium tripolyphosphate,STTP)여취병희산대단층중조Ⅰ형효원모형적방생광화유도작용,위STTP여취병희산재아본질화골효원광화유도적확전응용제공의거.방법 채용우표피Ⅰ형효원재원형개파편여투사전경얼망표면건립단층중조효원모형.연합응용조양본경함STTP여취병희산적모의체액-규산염수문정수지광화계통유도광화.재광화유도제12、24、48화72h취출연합응용조양본(매개시간점양본량위18),용소묘전경화투사전경정성분석효원미관형태학변화,병여광화유도72h、불함STTP화취병희산적공백조(양본량위18),이급부함STTP혹취병희산적대조조(STTP조、취병희산조,양본량균위18)진행비교.결과 공백조화2개대조조균미견효원섬유내광화현상.연합응용조재STTP여취병희산적협동유도하광화12 ～24 h후,중조효원섬유표면가견납미급구형전자치밀물침적;수착광화시간연장,섬유표면무정형전자치밀물축점소실,대지이효원섬유내정고도질서배렬적린회석납미미정,사섬유정현전형적주기성횡문결구.결론 재모의체액-규산염수문정수지존재적정황하,연합응용STTP화취병희산능성공유도린회석납미미정재불함비효원단백적중조효원내유서침적,실현효원섬유내광화.
Objective To investigate the functions of sodium tripolyphosphate (STTP) and polyacrylic acid (PAA) in the process of collagen biomimetic mineralization.This would allow future applications to other collagen matrices such as bone collagen or 3-D collagen scaffolds.Methods Glass cover slips and transmission electron microscopy (TEM) grids were coated with reconstituted type Ⅰ collagen fibrils.Mineralization of the reconstituted collagens was demonstrated with scanning electron microscopy (SEM) and TEM using a Portland cement-containing resin composite and a phosphate-containing fluid in the presence of PAA and STTP.The rest were immersed in a biomimetic remineralization medium without PAA and/or STTP (control).Results In the presence of PAA and STTP in the mineralization medium,intrafibrillar mineralization based on the non-classical crystallisation pathway could be identified.Mineral phases were evident within the collagen fibrils as early as 12 h after the initially-formed amorphous calcium phosphate nanoprecursors were transformed into apatite nanocrystals.Collagens at 72 h were heavily mineralized with periodically arranged intrafibrillar apatite platelets.Conversely,only large mineral spheres with no preferred association with collagen fibrils were observed in the absence of biomimetic analogues in the medium (control).Conclusions Intrafibrillar apatite deposition can be achieved via biomimetic mineralization system containing PAA and STTP when amorphous calcium phosphate precursor is stabilized.