CAJ | 학술논문

目的检测纳米银涂层改性义齿基托的细胞毒性及抗菌性能,为纳米银涂层的临床应用提供依据.方法根据基托树脂表面是否进行银离子溅射分为涂层组(根据离子溅射作用时间分为10、20、40、60、80、100 s涂层组)与基托对照组.用扫描电镜观察各组表面微观形貌,能谱分析表面元素;甲基噻唑基四唑比色分析涂层组、基托对照组、阴性对照组(聚乙烯)及阳性对照组(含50％二月桂酸二丁基锡的聚氯乙烯)与L929细胞共培养24、48、72 h的细胞相对增殖率及细胞毒性等级(每组每个时间点样本量为6);贴膜法检测纳米银涂层对变形链球菌及白色念珠菌的抗菌性能.结果扫描电镜观察发现基托对照组试件表面光滑,各涂层组试件表面可见纳米银颗粒致密均匀地分布于基托树脂表面,颗粒呈圆形,直径10 nm.甲基噻唑基四唑比色法结果显示各涂层组试件与L929细胞共培养24、48、72 h后的细胞相对增殖率均高于100％,细胞毒性等级均为0级.贴膜法结果显示基托对照组均有菌落存在,而各涂层组均无菌落出现.结论用离子溅射法可成功在义齿基托表面制备纳米银涂层,各涂层组材料均无细胞毒性,纳米银涂层改性义齿基托材料具有良好的抗菌性.
목적 검측납미은도층개성의치기탁적세포독성급항균성능,위납미은도층적림상응용제공의거.방법 근거기탁수지표면시부진행은리자천사분위도층조(근거리자천사작용시간분위10、20、40、60、80、100 s도층조)여기탁대조조.용소묘전경관찰각조표면미관형모,능보분석표면원소;갑기새서기사서비색분석도층조、기탁대조조、음성대조조(취을희)급양성대조조(함50％이월계산이정기석적취록을희)여L929세포공배양24、48、72 h적세포상대증식솔급세포독성등급(매조매개시간점양본량위6);첩막법검측납미은도층대변형련구균급백색념주균적항균성능.결과 소묘전경관찰발현기탁대조조시건표면광활,각도층조시건표면가견납미은과립치밀균균지분포우기탁수지표면,과립정원형,직경10 nm.갑기새서기사서비색법결과현시각도층조시건여L929세포공배양24、48、72 h후적세포상대증식솔균고우100％,세포독성등급균위0급.첩막법결과현시기탁대조조균유균락존재,이각도층조균무균락출현.결론 용리자천사법가성공재의치기탁표면제비납미은도층,각도층조재료균무세포독성,납미은도층개성의치기탁재료구유량호적항균성.
Objective To investigate celluar toxicity and antibacterial property of nano-silver coating modified denture base and to provide a theortical basis for clinical application.Method The samples were divided into coating group and denture base group.Samples in coating groups were treated with silver ion sputtering and were further divided into the 10,20,40,60,80,100 s subgroups according to their sputtering time.Surface microstructure of samples in coating groups and denture base group were observed by scanning electron microscopy (SEM),and composition of material surface were determined with energy dispersive spectrum (EDS) analysis.Methyl thiazolyl terazolium(MTT) method was used to analysis L929 cells proliferation rate and cell toxicity grade while the coating groups(n =6) and group test specimens (n =6),negative control (polyethylene,PE) (n =6) and positive control group (PVC-org.Sn) (n =6) were co-cultured with L929 cells.The antibacterial properties of coating groups were investigated by using the bacterial membrane adhering method with Streptococcus mutans and Candida albicans.Results SEM observation showed that the surface of control group specimens were smooth,whereas dense and uniform distributed nano silver particles were visible on the surface of coating group specimens.The silver particles were round,and the diameter size was 10 nm.MTT assay showed that after being co-cultured with specimens in control group,L929 cell had higher proliferation rate than 100％ at 24,48,72 h,and cytotoxicity were graded 0.The membrane adhering method results showed that all of bacterial colonies were found in control group,but without anything at the coating groups.Conclusions Deposition of nano silver on the denture base surface by ion sputtering method is a viable method.There's no cytotoxicity of series Ag-coating material.Nano-silver modified denture base material benefits L929 cell surface proliferation and has antibacterial action.