中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
4期
244-246
,共3页
王丹杨%张凌%李芳%陈宇江%陈吉华
王丹楊%張凌%李芳%陳宇江%陳吉華
왕단양%장릉%리방%진우강%진길화
牙本质%基质金属蛋白酶2%液相芯片技术
牙本質%基質金屬蛋白酶2%液相芯片技術
아본질%기질금속단백매2%액상심편기술
Dentin%Matrix metalloproteinase 2%Fluorescent microsphere immunoassay
目的 定量检测青年人群正常牙本质中基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)的含量及分布情况.方法 选择离体前磨牙30颗和第三磨牙27颗(患者年龄20 ～ 30岁),纵切成1.5 mm厚的牙本质片,磨除釉质后将冠部牙本质分成浅层和深层两部分,分别制备成牙本质粉,脱矿、提取蛋白后利用液相芯片技术定量检测MMP-2含量.结果 青年人群前磨牙牙本质浅层和深层MMP-2分别为(0.022±0.006)和(2.087±0.090) ng/mg,磨牙牙本质浅层和深层MMP-2含量分别为(0.336±0.037)和(3.312±0.308) ng/mg;各牙位深层牙本质MMP-2含量均显著高于浅层,磨牙同层次牙本质MMP-2含量均显著高于前磨牙(P＜0.05).结论 健康青年正常牙本质中含MMP-2,不同牙位及牙本质层次MMP-2含量不同.
目的 定量檢測青年人群正常牙本質中基質金屬蛋白酶2(matrix metalloproteinase-2,MMP-2)的含量及分佈情況.方法 選擇離體前磨牙30顆和第三磨牙27顆(患者年齡20 ～ 30歲),縱切成1.5 mm厚的牙本質片,磨除釉質後將冠部牙本質分成淺層和深層兩部分,分彆製備成牙本質粉,脫礦、提取蛋白後利用液相芯片技術定量檢測MMP-2含量.結果 青年人群前磨牙牙本質淺層和深層MMP-2分彆為(0.022±0.006)和(2.087±0.090) ng/mg,磨牙牙本質淺層和深層MMP-2含量分彆為(0.336±0.037)和(3.312±0.308) ng/mg;各牙位深層牙本質MMP-2含量均顯著高于淺層,磨牙同層次牙本質MMP-2含量均顯著高于前磨牙(P＜0.05).結論 健康青年正常牙本質中含MMP-2,不同牙位及牙本質層次MMP-2含量不同.
목적 정량검측청년인군정상아본질중기질금속단백매2(matrix metalloproteinase-2,MMP-2)적함량급분포정황.방법 선택리체전마아30과화제삼마아27과(환자년령20 ～ 30세),종절성1.5 mm후적아본질편,마제유질후장관부아본질분성천층화심층량부분,분별제비성아본질분,탈광、제취단백후이용액상심편기술정량검측MMP-2함량.결과 청년인군전마아아본질천층화심층MMP-2분별위(0.022±0.006)화(2.087±0.090) ng/mg,마아아본질천층화심층MMP-2함량분별위(0.336±0.037)화(3.312±0.308) ng/mg;각아위심층아본질MMP-2함량균현저고우천층,마아동층차아본질MMP-2함량균현저고우전마아(P＜0.05).결론 건강청년정상아본질중함MMP-2,불동아위급아본질층차MMP-2함량불동.
Objective To compare the distribution and concentration of matrix metalloproteinases-2 (MMP-2) in different dentin depth of premolar and molar of young people.Methods Freshly extracted human premolars and molars (aged between 20-30) were sectioned to 1.5 mm thick slices along the longitudinal axis of the tooth separately.Enamel and pulp of each slice was removed,and then the premolar and molar slices were respectively divided into two subgroups according to superficial or deep dentin and pulverized to fine powder.After dentin protein was extracted,the concentrations of MMP-2 in different tooth were detected using fluorescent microsphere immunoassay.Results The content of MMP-2 in superficial layer dentin of premolar was (0.022 ± 0.006) ng/mg.The content of MMP-2 in deep layer dentin of premolar was (2.087 ± 0.090) ng/mg.The content of MMP-2 in superficial layer dentin of molar was (0.336 ± 0.037) ng/mg.The content of MMP-2 in deep layer dentin of molar was (3.312 ± 0.308) ng/mg.Conclusions MMP-2 exists in human coronal dentin.In the same type of teeth of young people,the concentration of MMP-2 in deep dentin was significant higher than those in superficial dentin.In the same dentin depth,the concentration of MMP-2 in molar was significant higher than those in premolar.
