CAJ | 학술논문

目的探讨偏侧咀嚼大鼠咬肌中过氧化物酶体增殖物受体γ共激活因子1α(peroxisome proliferator-activated receptor-γcoactivator-1α,PGC-1α)表达及肌细胞凋亡与咀嚼肌变化的机制.方法将36只Wistar雌性大鼠用随机数字表法分成2、4、6、8周4个时间点,每一时间点9只,其中6只作为建模组,拔除上颌左侧磨牙建立偏侧咀嚼动物模型,3只作为对照组.应用原子吸收分光光度法检测各组Ca2+含量;用实时荧光定量PCR法检测咬肌组织中PGC-1α mRNA的相对表达量;用Hoechst染色检测细胞凋亡.结果建模早期建模组大鼠拔牙侧Ca2+含量升高且高于对照组,4周时达峰值[(43.62±2.36) μg/g];4周时建模组PGC-1α mRNA的相对表达量达峰值[拔牙侧(1.57±0.10)、非拔牙侧(1.92±0.06)],6、8周时表达量逐渐下降[拔牙侧(1.06±0.08)、(1.08±0.07),非拔牙侧(1.09±0.10)、(1.11±0.08)];非拔牙侧细胞凋亡率随时间延长而上升,并在6周时达峰值[(38.56±1.64)％].结论 PGC-1α和肌细胞凋亡参与偏侧咀嚼后咬肌的组织改建,并在偏侧咀嚼不同时期发挥作用.
목적 탐토편측저작대서교기중과양화물매체증식물수체γ공격활인자1α(peroxisome proliferator-activated receptor-γcoactivator-1α,PGC-1α)표체급기세포조망여저작기변화적궤제.방법 장36지Wistar자성대서용수궤수자표법분성2、4、6、8주4개시간점,매일시간점9지,기중6지작위건모조,발제상합좌측마아건립편측저작동물모형,3지작위대조조.응용원자흡수분광광도법검측각조Ca2+함량;용실시형광정량PCR법검측교기조직중PGC-1α mRNA적상대표체량;용Hoechst염색검측세포조망.결과 건모조기건모조대서발아측Ca2+함량승고차고우대조조,4주시체봉치[(43.62±2.36) μg/g];4주시건모조PGC-1α mRNA적상대표체량체봉치[발아측(1.57±0.10)、비발아측(1.92±0.06)],6、8주시표체량축점하강[발아측(1.06±0.08)、(1.08±0.07),비발아측(1.09±0.10)、(1.11±0.08)];비발아측세포조망솔수시간연장이상승,병재6주시체봉치[(38.56±1.64)％].결론 PGC-1α화기세포조망삼여편측저작후교기적조직개건,병재편측저작불동시기발휘작용.
Objective To investigate the changes of peroxisome proliferator-activated receptor-γ coactivator-1 α (PGC-1 α) mRNA and cytoapoptosis in the rats' masseter muscle which had been influenced by unilateral chewing,and to explore the theoretical foundation of changes in masticatory muscles induced by unilateral chewing.Methods The animal models were established by extracting the Wistar rats' left maxillary molars.Thirty-six female Wistar rats were randomly divided into four groups of 2,4,6 and 8 weeks,nine each.In each group there were six rats with molar extracted and three as control.The Ca2+ level was detected by atomic spectrophotometric method.The relative expression of PGC-1α mRNA was detected by real-time fluorescent quantitative PCR.The apoptosis index was detected by Hoechst staining.Results The Ca2+ level in the muscle on the extraction side were significantly higher than that in the controls in the beginning stage of unilateral chewing,and reached the peak at the 4th week [(43.62 ± 2.36) μg/g].The relative expressions of PGC-1α increased from the beginning and reached the maximum level at the 4th week [extraction side:(1.57±0.10); non-extraction side:(1.92±0.06)],while the relative expressions of PGC-1α in 6 and 8 weeks decreased gradually [extraction side:(1.06 ±0.08),(1.08 ±0.07); non-extraction side:(1.09±0.10),(1.11 ± 0.08)].The changes of apoptosis index on non-extraction side increased continually and peaked at the 6th week [(38.56± 1.64)％].Conclusions PGC-1α and cytoapoptosis played important roles in different stages of tissue remodeling induced by unilateral chewing.