中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
8期
464-469
,共6页
钱雅洁%蒋文晖%王翔%段宁%黄晓峰%王文梅
錢雅潔%蔣文暉%王翔%段寧%黃曉峰%王文梅
전아길%장문휘%왕상%단저%황효봉%왕문매
念珠菌,白色%NF-κB%β-防御素%白斑,口腔%核苷酸结合寡聚化结构域1
唸珠菌,白色%NF-κB%β-防禦素%白斑,口腔%覈苷痠結閤寡聚化結構域1
념주균,백색%NF-κB%β-방어소%백반,구강%핵감산결합과취화결구역1
Candida albicans%NF-kappa B%Beta-defensins%Leukoplakia,oral%Nucleotide-binding oligomerization domain 1
目的 研究白色念珠菌白斑和无白色念珠菌感染的白斑患者组织中核苷酸结合寡聚化结构域1 (nucleotide-binding oligomerization domain 1,NOD1)、核因子κB及人β-防御素1,2,3的表达,探讨白色念珠菌感染对口腔白斑组织中NOD1信号通路内关键蛋白和人β-防御素表达的影响.方法 收集40例口腔黏膜白斑组织样本,采用HE染色、过碘酸-雪夫(periodic acid-Schiff,PAS)染色、真菌银染法和免疫组化法检测,四项均阳性者19例,判定为白色念珠菌白斑,其余21例判定为无白色念珠菌感染白斑.采用蛋白质印迹法检测白色念珠菌白斑和无白色念珠菌感染的白斑组织中NOD1和核因子κB的表达水平并进行比较.采用免疫组化法检测白色念珠菌白斑和无白色念珠菌感染的白斑组织中NOD1、核因子κB和人β-防御素1,2,3的表达,图像分析测定平均吸光度值,对平均吸光度值行独立样本t检验,检验水准为双侧α=0.05.结果 在40例口腔白斑中,白色念珠菌阳性率为48％(19/40).蛋白质印迹法结果显示白色念珠菌白斑中NOD1、核因子κB蛋白表达水平较无白色念珠菌感染的白斑显著降低.免疫组化结果表明,无白色念珠菌感染的白斑中NOD1、核因子κB和人β-防御素1,2,3染色的吸光度值分别为0.31 ±0.02、0.47±0.03、0.42±0.02、0.53±0.04、0.47±0.03,白色念珠菌白斑中NOD1、核因子κB和人β-防御素1,2,3染色的吸光度值分别为0.25±0.01、0.30±0.02、0.35±0.02、0.42±0.03、0.36±0.02,白色念珠菌白斑组织中NOD1、核因子κB和人β-防御素1,2,3的表达水平均较无白色念珠菌感染组显著减弱,差异均有统计学意义(P＜0.05).结论 白色念珠菌感染可能通过抑制NOD1信号通路降低口腔白斑组织中人β-防御素的表达.
目的 研究白色唸珠菌白斑和無白色唸珠菌感染的白斑患者組織中覈苷痠結閤寡聚化結構域1 (nucleotide-binding oligomerization domain 1,NOD1)、覈因子κB及人β-防禦素1,2,3的錶達,探討白色唸珠菌感染對口腔白斑組織中NOD1信號通路內關鍵蛋白和人β-防禦素錶達的影響.方法 收集40例口腔黏膜白斑組織樣本,採用HE染色、過碘痠-雪伕(periodic acid-Schiff,PAS)染色、真菌銀染法和免疫組化法檢測,四項均暘性者19例,判定為白色唸珠菌白斑,其餘21例判定為無白色唸珠菌感染白斑.採用蛋白質印跡法檢測白色唸珠菌白斑和無白色唸珠菌感染的白斑組織中NOD1和覈因子κB的錶達水平併進行比較.採用免疫組化法檢測白色唸珠菌白斑和無白色唸珠菌感染的白斑組織中NOD1、覈因子κB和人β-防禦素1,2,3的錶達,圖像分析測定平均吸光度值,對平均吸光度值行獨立樣本t檢驗,檢驗水準為雙側α=0.05.結果 在40例口腔白斑中,白色唸珠菌暘性率為48％(19/40).蛋白質印跡法結果顯示白色唸珠菌白斑中NOD1、覈因子κB蛋白錶達水平較無白色唸珠菌感染的白斑顯著降低.免疫組化結果錶明,無白色唸珠菌感染的白斑中NOD1、覈因子κB和人β-防禦素1,2,3染色的吸光度值分彆為0.31 ±0.02、0.47±0.03、0.42±0.02、0.53±0.04、0.47±0.03,白色唸珠菌白斑中NOD1、覈因子κB和人β-防禦素1,2,3染色的吸光度值分彆為0.25±0.01、0.30±0.02、0.35±0.02、0.42±0.03、0.36±0.02,白色唸珠菌白斑組織中NOD1、覈因子κB和人β-防禦素1,2,3的錶達水平均較無白色唸珠菌感染組顯著減弱,差異均有統計學意義(P＜0.05).結論 白色唸珠菌感染可能通過抑製NOD1信號通路降低口腔白斑組織中人β-防禦素的錶達.
목적 연구백색념주균백반화무백색념주균감염적백반환자조직중핵감산결합과취화결구역1 (nucleotide-binding oligomerization domain 1,NOD1)、핵인자κB급인β-방어소1,2,3적표체,탐토백색념주균감염대구강백반조직중NOD1신호통로내관건단백화인β-방어소표체적영향.방법 수집40례구강점막백반조직양본,채용HE염색、과전산-설부(periodic acid-Schiff,PAS)염색、진균은염법화면역조화법검측,사항균양성자19례,판정위백색념주균백반,기여21례판정위무백색념주균감염백반.채용단백질인적법검측백색념주균백반화무백색념주균감염적백반조직중NOD1화핵인자κB적표체수평병진행비교.채용면역조화법검측백색념주균백반화무백색념주균감염적백반조직중NOD1、핵인자κB화인β-방어소1,2,3적표체,도상분석측정평균흡광도치,대평균흡광도치행독립양본t검험,검험수준위쌍측α=0.05.결과 재40례구강백반중,백색념주균양성솔위48％(19/40).단백질인적법결과현시백색념주균백반중NOD1、핵인자κB단백표체수평교무백색념주균감염적백반현저강저.면역조화결과표명,무백색념주균감염적백반중NOD1、핵인자κB화인β-방어소1,2,3염색적흡광도치분별위0.31 ±0.02、0.47±0.03、0.42±0.02、0.53±0.04、0.47±0.03,백색념주균백반중NOD1、핵인자κB화인β-방어소1,2,3염색적흡광도치분별위0.25±0.01、0.30±0.02、0.35±0.02、0.42±0.03、0.36±0.02,백색념주균백반조직중NOD1、핵인자κB화인β-방어소1,2,3적표체수평균교무백색념주균감염조현저감약,차이균유통계학의의(P＜0.05).결론 백색념주균감염가능통과억제NOD1신호통로강저구강백반조직중인β-방어소적표체.
Objective To examine the expression of nucleotide-binding oligomerization domain 1 (NOD1),nuclear factor-kappa B(NF-κB) and human beta-defensins in candidal albicans leukoplakia and to investigate the effect of candida albicans infection on key proteins in NOD 1 signaling pathway and the expression of human beta-defensin.Methods Forty cases of oral leukoplakia samples were collected and stained by hematoxylin-eosin staining,periodic acid-Schiff staining,silver staining and immunohistochemical methods.Nineteen samples were positive with these four methods and judged as candidal albicans leukoplakia,and the other twenty-one samples judged as leukoplakia without candidal albicans infection.Western blotting was used to detect the expressions of NOD1 and NF-κB in these forty samples.In addition,the immunohistochemical method was adopted to investigate the relationship between NOD 1,NF-κB,human beta-defensin 1,2,3 expressions and candida albicans.Results The positive rate of candida albicans in oral leukoplakia was 48％(19/40).The expressions of NOD1 and NF-κB in the candida albicans leukoplakia were lower than that in leukoplakia without candida albicans infection.The mean optical density value of NOD 1,NF-κB,human beta-defensin 1,2,3 in candidal albicans leukoplakia were 0.25±0.01,0.30±0.02,0.35±0.02,0.42±0.03,0.36±0.02 respectively,which were significantly lower than that in leukoplakia without candida albicans infection (0.31±0.02,0.47±0.03,0.42±0.02,0.53±0.04,0.47±0.03) (P＜0.05).Conclusions By inhibiting the NOD1 signaling pathway,candida albicans infection may reduce the expression level of human beta-defensin 1,2,3 in oral leukoplakia.
