CAJ | 학술논문

目的探讨Toll样受体4(Toll-like receptor-4,TLR-4)与大鼠颞下颌关节(temporomandibular joint,TMJ)滑膜炎的相关性.方法选取60只雄性Wistar大鼠,通过随机数字表随机分为5组,每组12只.A组(空白对照组):正常饮食,不做任何处理;B组(咬肌切除组):切除大鼠双侧咬肌;C组(咬合干扰组):右下第一磨牙粘接铸造金属冠;D组(咬合升高组):粘接上颌磨牙(牙合)垫;E组(咬肌切除加咬合升高组):切除双侧咬肌并粘接上颌磨牙(牙合)垫.取右侧TMJ作为样本,HE染色观察滑膜的变化并进行病理学评分,免疫组化染色检测TLR-4蛋白表达,实时定量PCR检测TLR-4mRNA表达,分别对TLR-4蛋白及mRNA表达与病理学评分进行Spearman相关性分析.结果 A～E组的病理学评分分别为0.5 ±0.5、2.5±1.0、2.7± 1.0、3.0± 0.9、5.3± 1.2,TLR-4蛋白表达分别为(3.2±1.5)％、(16.0±2.6)％、(15.8±2.1)％、(17.5±2.4)％、(38.2±4.4)％,TLR-4 mRNA表达分别为1.07±0.09、2.12±0.33、2.07±0.29、2.17±0.34、4.53±0.46.与A组相比,B～E组病理学评分、TLR-4蛋白和TLR-4 mRNA表达均显著升高(P＜0.05),TLR-4蛋白与病理学评分显著相关(r=0.785,P＜0.05),TLR-4mRNA表达与病理学评分显著相关(r=0.720,P＜0.05).结论 TLR-4可能参与了大鼠TMJ滑膜炎的发病过程.
목적 탐토Toll양수체4(Toll-like receptor-4,TLR-4)여대서섭하합관절(temporomandibular joint,TMJ)활막염적상관성.방법 선취60지웅성Wistar대서,통과수궤수자표수궤분위5조,매조12지.A조(공백대조조):정상음식,불주임하처리;B조(교기절제조):절제대서쌍측교기;C조(교합간우조):우하제일마아점접주조금속관;D조(교합승고조):점접상합마아(아합)점;E조(교기절제가교합승고조):절제쌍측교기병점접상합마아(아합)점.취우측TMJ작위양본,HE염색관찰활막적변화병진행병이학평분,면역조화염색검측TLR-4단백표체,실시정량PCR검측TLR-4mRNA표체,분별대TLR-4단백급mRNA표체여병이학평분진행Spearman상관성분석.결과 A～E조적병이학평분분별위0.5 ±0.5、2.5±1.0、2.7± 1.0、3.0± 0.9、5.3± 1.2,TLR-4단백표체분별위(3.2±1.5)％、(16.0±2.6)％、(15.8±2.1)％、(17.5±2.4)％、(38.2±4.4)％,TLR-4 mRNA표체분별위1.07±0.09、2.12±0.33、2.07±0.29、2.17±0.34、4.53±0.46.여A조상비,B～E조병이학평분、TLR-4단백화TLR-4 mRNA표체균현저승고(P＜0.05),TLR-4단백여병이학평분현저상관(r=0.785,P＜0.05),TLR-4mRNA표체여병이학평분현저상관(r=0.720,P＜0.05).결론 TLR-4가능삼여료대서TMJ활막염적발병과정.
Objective To investigate the expression of the Toll-like receptor-4 (TLR-4) in temporomandibular joint synovitis in rats,and to discuss the correlation between the expression of TLR-4 and the synovitis.Methods Sixty male wistar rats were randomly divided into five groups,12 each.Group A was the control group in which the rats were given normal diet.In Group B,the rats' bilateral masseter muscles were cut off (masseter resection group).In Group C,An cast metal crown were bonded on the mandibular right first molar of each rat (occlusal interference group).In Group D,occlusal pad were bonded on maxillary molars of each rat (occlusal dimension increase group).In Group E,rats' bilateral masseter muscles were resected and occlusal pads were bonded on their maxillary molars (masseter resection and occlusal dimension increase group).Pathological changes of synovium were observed using hematoxylin and eosin (HE) stains and pathology scores were evaluated.The expression of TLR-4 were determined by immunohistochemical stains,and the expression of TLR-4 mRNA were determined by real-time PCR.The correlation between the expression of TLR-4,TLR-4 mRNA and the pathological score were analyzed using Spearman analysis.Results The pathological scores of Group A-E were 0.5±0.5,2.5±1.0,2.7±1.0,3.0±0.9,5.3±1.2 respectively.The expression of TLR-4 were (3.2±1.5) ％,(16.0±2.6) ％,(15.8±2.1) ％,(17.5±2.4) ％,(38.2±4.4) ％.The expression of TLR-4 mRNA were 1.07±0.09,2.12±0.33,2.07±0.29,2.17±0.34,4.53±0.46.Compared with group A,groups B-E showed significant higher pathology score (P＜0.05) and increased expression of both TLR-4 (P＜0.05) and TLR-4 mRNA (P＜0.05).An significant positive correlation was found between the expression of TLR-4 and the pathology score (r=0.785,P＜0.05),and between the expression of TLR-4 mRNA and the pathology score (r=0.720,P＜0.05).Conclusions TLR-4 may be closely associated with the development of the synovitis of TMJ of rats.