CAJ | 학술논문

目的研究整合牛血清白蛋白(bovine serum albumin,BSA)后钙磷涂层理化性能的变化,为制备仿生钙磷涂层、探索生长因子的释放行为提供实验基础.方法将纯钛试件浸入不含BSA(对照组)及含1、10、100mg/LBSA的饱和钙磷溶液中,采用仿生共沉积的方法,形成BSA-钙磷涂层,检测涂层形貌、物相组成、化学官能团,通过BCA蛋白浓度测定试剂盒,定量检测共沉积至涂层的BSA含量,并绘制体外释放曲线.结果通过仿生共沉积法可成功将BSA整合于钙磷涂层内部;BSA-钙磷涂层主要由羟基磷灰石和磷酸八钙组成;随着饱和钙磷溶液中BSA质量浓度增加,涂层中片状晶体结构转变为细小的网格状结构;1、10、100 mg/L BSA组涂层中BSA的整合率分别为(72.4±2.4)％、(62.3±0.9)％、(42.2±1.7)％,24h和30 d的BSA累积释放量分别为(1.57±0.09)、(8.82±0.93)、(140.24±3.12) μg和(2.39±0.29)、(14.39±0.70)、(151.06±2.00) μg.结论仿生共沉积法制备的BSA-钙磷涂层可成为有效的BSA载体;BSA的质量浓度可影响其整合率和释放量,BSA质量浓度为10 mg/L时可获得较理想的整合率及体外释放行为.
목적 연구정합우혈청백단백(bovine serum albumin,BSA)후개린도층이화성능적변화,위제비방생개린도층、탐색생장인자적석방행위제공실험기출.방법 장순태시건침입불함BSA(대조조)급함1、10、100mg/LBSA적포화개린용액중,채용방생공침적적방법,형성BSA-개린도층,검측도층형모、물상조성、화학관능단,통과BCA단백농도측정시제합,정량검측공침적지도층적BSA함량,병회제체외석방곡선.결과 통과방생공침적법가성공장BSA정합우개린도층내부;BSA-개린도층주요유간기린회석화린산팔개조성;수착포화개린용액중BSA질량농도증가,도층중편상정체결구전변위세소적망격상결구;1、10、100 mg/L BSA조도층중BSA적정합솔분별위(72.4±2.4)％、(62.3±0.9)％、(42.2±1.7)％,24h화30 d적BSA루적석방량분별위(1.57±0.09)、(8.82±0.93)、(140.24±3.12) μg화(2.39±0.29)、(14.39±0.70)、(151.06±2.00) μg.결론 방생공침적법제비적BSA-개린도층가성위유효적BSA재체;BSA적질량농도가영향기정합솔화석방량,BSA질량농도위10 mg/L시가획득교이상적정합솔급체외석방행위.
Objective To study the incorporation rate and release behavior of bovine serum albumin(BSA) incorporated into the calcium phosphate coating by biomimetic deposition,as well as the physical and chemical properties of the hybrid coating,and to provide experimental basis for the fabrication of growth factor/biomimetic calcium phosphate coating and exploration for the loading/release behavior of growth factors.Methods Pure titanium specimens were immersed into saturated calcium phosphate solutions(SCP) containing no BSA(controlled group) and 3 different concentrations of BSA(experimental groups):1,10 and 100 mg/L.Biomimetic calcium phosphate coating was formed on titanium surface and BSA was incorporated into the coating through co-deposition.The topography of the specimen was observed using scanning electron microscopy(SEM).Chemical structure and phase composition of coatings were detected by Fourier infrared spectroscopy(FTIR) analysis and X-ray diffraction(XRD) respectively.BSA incorporation rate and release profile were determined by bicinchoninic acid protein assay kit.Results The biomimetic calcium phosphate coating was mainly composed of hydroxyapatite and octacalcium phosphate.BSA was successfully incorporated into the calcium phosphate coatings in all the 3 experimental groups.With the increase of BSA concentration,plate-like units of the coatings were turned into small grid structure.BSA incorporation rates of the three experimental groups were(72.4 ± 2.4)％,(62.3 ± 0.9)％ and (42.2 ± 1.7)％ respectively.The in vitro release test showed that all three BSA release profiles could be divided into two significant different stages:early burst release stage and later sustained release stage.The amount of BSA release of the 3 experimental groups in 24 h and 30 d were (1.57±0.09),(8.82±0.93),(140.24±3.12) μg,and (2.39±0.29),(14.39±0.70),(151.06±2.00) μg respectively.Conclusions Biomimetic calcium phosphate coating can be used as an effective carrier for protein.BSA concentration has an impact on the incorporation rate and release speed of BSA from the calcium phosphate coating.Favorable BSA incorporation rate and release behavior can be obtained at BSA concentration of 10 mg/L.