中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
10期
596-599
,共4页
咀嚼肌%痛觉过敏%牙(牙合),创伤性%动物实验
咀嚼肌%痛覺過敏%牙(牙閤),創傷性%動物實驗
저작기%통각과민%아(아합),창상성%동물실험
Masticatory muscles%Hyperalgesia%Dental occlusion,traumatic%Animal experimentation
目的 探讨(牙合)干扰去除时间与大鼠咀嚼肌机械痛觉过敏恢复的关系,为临床咬合治疗时机的选择提供实验依据.方法 通过对雄性SD大鼠上颌第一磨牙粘固厚度为0.4 mm全冠的方法施加(牙合)干扰,建立大鼠(牙合)干扰模型,分别在建模后2、3、4、5、6d去除(牙合)干扰,并设阳性对照组(不去除(牙合)干扰)及阴性对照组(假干扰),共7组,每组5只大鼠.建模后1、3、5、7、10、14、21、28 d观察各组大鼠双侧颞肌和咬肌机械痛觉过敏的情况,即测定机械刺激反应阈值.结果 阳性对照组大鼠施加(牙合)干扰后大鼠双侧咀嚼肌机械刺激反应阈值持续降低,7d达到最低点,之后部分回升,各时间点与阴性对照组间差异均有统计学意义(P＜0.05).去除(牙合)干扰后各组大鼠双侧咀嚼肌机械刺激反应阈值逐渐恢复,2、3、4、5d去除干扰组大鼠双侧咀嚼肌机械刺激反应阈值分别在建模后10、14、14、21d与阴性对照组差异无统计学意义(P＞0.05),且左右侧咀嚼肌机械刺激反应阈值差异均无统计学意义(P＞0.05).6d去除干扰组大鼠双侧咀嚼肌机械刺激反应阈值有一定回升,但28 d时[右侧颞肌和咬肌分别为(1.365±0.018)和(1.437±0.024)N]仍显著低于阴性对照组[右侧颞肌和咬肌分别为(1.554±0.040)和(1.546±0.019) N](P＜0.001).结论 (牙合)干扰后5d内去除干扰,大鼠咀嚼肌机械痛觉过敏可恢复正常,且去除干扰的时间越早,恢复得越快.
目的 探討(牙閤)榦擾去除時間與大鼠咀嚼肌機械痛覺過敏恢複的關繫,為臨床咬閤治療時機的選擇提供實驗依據.方法 通過對雄性SD大鼠上頜第一磨牙粘固厚度為0.4 mm全冠的方法施加(牙閤)榦擾,建立大鼠(牙閤)榦擾模型,分彆在建模後2、3、4、5、6d去除(牙閤)榦擾,併設暘性對照組(不去除(牙閤)榦擾)及陰性對照組(假榦擾),共7組,每組5隻大鼠.建模後1、3、5、7、10、14、21、28 d觀察各組大鼠雙側顳肌和咬肌機械痛覺過敏的情況,即測定機械刺激反應閾值.結果 暘性對照組大鼠施加(牙閤)榦擾後大鼠雙側咀嚼肌機械刺激反應閾值持續降低,7d達到最低點,之後部分迴升,各時間點與陰性對照組間差異均有統計學意義(P＜0.05).去除(牙閤)榦擾後各組大鼠雙側咀嚼肌機械刺激反應閾值逐漸恢複,2、3、4、5d去除榦擾組大鼠雙側咀嚼肌機械刺激反應閾值分彆在建模後10、14、14、21d與陰性對照組差異無統計學意義(P＞0.05),且左右側咀嚼肌機械刺激反應閾值差異均無統計學意義(P＞0.05).6d去除榦擾組大鼠雙側咀嚼肌機械刺激反應閾值有一定迴升,但28 d時[右側顳肌和咬肌分彆為(1.365±0.018)和(1.437±0.024)N]仍顯著低于陰性對照組[右側顳肌和咬肌分彆為(1.554±0.040)和(1.546±0.019) N](P＜0.001).結論 (牙閤)榦擾後5d內去除榦擾,大鼠咀嚼肌機械痛覺過敏可恢複正常,且去除榦擾的時間越早,恢複得越快.
목적 탐토(아합)간우거제시간여대서저작기궤계통각과민회복적관계,위림상교합치료시궤적선택제공실험의거.방법 통과대웅성SD대서상합제일마아점고후도위0.4 mm전관적방법시가(아합)간우,건립대서(아합)간우모형,분별재건모후2、3、4、5、6d거제(아합)간우,병설양성대조조(불거제(아합)간우)급음성대조조(가간우),공7조,매조5지대서.건모후1、3、5、7、10、14、21、28 d관찰각조대서쌍측섭기화교기궤계통각과민적정황,즉측정궤계자격반응역치.결과 양성대조조대서시가(아합)간우후대서쌍측저작기궤계자격반응역치지속강저,7d체도최저점,지후부분회승,각시간점여음성대조조간차이균유통계학의의(P＜0.05).거제(아합)간우후각조대서쌍측저작기궤계자격반응역치축점회복,2、3、4、5d거제간우조대서쌍측저작기궤계자격반응역치분별재건모후10、14、14、21d여음성대조조차이무통계학의의(P＞0.05),차좌우측저작기궤계자격반응역치차이균무통계학의의(P＞0.05).6d거제간우조대서쌍측저작기궤계자격반응역치유일정회승,단28 d시[우측섭기화교기분별위(1.365±0.018)화(1.437±0.024)N]잉현저저우음성대조조[우측섭기화교기분별위(1.554±0.040)화(1.546±0.019) N](P＜0.001).결론 (아합)간우후5d내거제간우,대서저작기궤계통각과민가회복정상,차거제간우적시간월조,회복득월쾌.
Objective To investigate the relationship between the existence of occlusal interference and masticatory muscle hyperalgesia by exploring the stimulus-response relationship between the duration of occlusal interference and masticatory muscle mechanical withdrawal threshold.Methods Occlusal interference with 0.4 mm-thick crowns on rat molars was removed under anaesthesia at 2,3,4,5,and 6 d after wear,and masticatory muscle mechanical withdrawal threshold was tested at 1,3,5,7,10,14,21 and 28 d.Results Decreased mechanical withdrawal thresholds were detected in temporal muscles and masseter muscles on both sides following occlusal interference(P＜0.05).After removal of crowns at 2,3,4 and 5 d,rats exhibited the similar head withdrawal thresholds as the sham-application control in masticatory muscles on both sides at 10,14,14 and 21 d(P＞0.05).No significant differences were detected between the contralateral side with the ipsilateral side(P＜0.05).After removal of crowns at 6 d,rats still exhibited significantly decreased head withdrawal thresholds[right temporal muscle:(1.365±0.018) N; right masseter:(1.437 ± 0.024) N] in masticatory muscles on both sides until the last day of the experiment[the sham-application control:right temporal muscle:(1.554± 0.040) N,P＜0.001; right masseter:(1.546± 0.019)N,P＜0.001].Conclusions The mechanical hyperalgesia can disappear after removal of the occlusal interference at 5 d,and the existence of the occlusal interference is positively correlated with the duration of the mechanical hyperalgesia.
