中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
10期
607-613
,共7页
王娟%唐志娟%李谨%张光东
王娟%唐誌娟%李謹%張光東
왕연%당지연%리근%장광동
根尖周炎%高通量核苷酸测序%生物膜
根尖週炎%高通量覈苷痠測序%生物膜
근첨주염%고통량핵감산측서%생물막
Periapical periodontitis%High-throughput nucleotide sequencing%Biofilms
目的 采用高通量焦磷酸测序技术比较根管治疗后伴或不伴根尖周炎患牙根管内微生物群落的构成差异,寻找与持续性根尖周感染相关的根管内可疑微生物.方法 临床采集根管治疗后伴根尖周炎患牙(伴根尖周炎组,10颗)和无根尖周炎患牙(无根尖周炎组,10颗)根管内微生物群落样本.提取样本中细菌的总DNA,PCR扩增其对应于16S rRNA片段上的V1～ V3高变区基因片段以构建宏基因组文库.经焦磷酸测序后进行种系发育分析、多样性分析和主成分分析(PcoA分析).结果 共检出132种细菌,分属于21个菌门和96个菌属.在门水平,伴根尖周炎组群落的主要菌门为厚壁菌门[32％(18 534/58 688)]、变形菌门[27％(15 626/58 688)]、放线菌门[15％(8 685/58 688)]、拟杆菌门[11％(6 163/58 688)]、梭杆菌门[8％(4 761/58 688)]和螺旋体门[3％(1 785/58 688)].无根尖周炎组群落的主要菌门为厚壁菌门[31％(16 941/55 480)]、变形菌门[27％(14 748/55 480)]、拟杆菌门[18％(9 948/55 480)]、梭杆菌门[10％(5 307/55 480)]、放线菌门[9％(4 761/55 480)]和绿弯菌门[3％(1 785/55 480)].在丰度方面,伴根尖周炎组群落中的放线菌门显著高于无根尖周炎组(P＜0.01).在检出率方面,放线菌在伴根尖周炎组和无根尖周炎组中分别是100％和50％.主成分分析结果表明各样本间的差异较大,但样本有根据病变状况(伴根尖周炎或无根尖周炎)聚类的趋势.结论 根管治疗后伴根尖周炎患牙的根管内微生物群落构成多样性较高,与无根尖周炎患牙在根管内微生物群落构成上有一定差异.可能存在某种根管内群落构成模式与持续性根尖周感染具有一定的相关性.放线菌与根管治疗后持续性根尖周感染可能具有一定的相关性.
目的 採用高通量焦燐痠測序技術比較根管治療後伴或不伴根尖週炎患牙根管內微生物群落的構成差異,尋找與持續性根尖週感染相關的根管內可疑微生物.方法 臨床採集根管治療後伴根尖週炎患牙(伴根尖週炎組,10顆)和無根尖週炎患牙(無根尖週炎組,10顆)根管內微生物群落樣本.提取樣本中細菌的總DNA,PCR擴增其對應于16S rRNA片段上的V1～ V3高變區基因片段以構建宏基因組文庫.經焦燐痠測序後進行種繫髮育分析、多樣性分析和主成分分析(PcoA分析).結果 共檢齣132種細菌,分屬于21箇菌門和96箇菌屬.在門水平,伴根尖週炎組群落的主要菌門為厚壁菌門[32％(18 534/58 688)]、變形菌門[27％(15 626/58 688)]、放線菌門[15％(8 685/58 688)]、擬桿菌門[11％(6 163/58 688)]、梭桿菌門[8％(4 761/58 688)]和螺鏇體門[3％(1 785/58 688)].無根尖週炎組群落的主要菌門為厚壁菌門[31％(16 941/55 480)]、變形菌門[27％(14 748/55 480)]、擬桿菌門[18％(9 948/55 480)]、梭桿菌門[10％(5 307/55 480)]、放線菌門[9％(4 761/55 480)]和綠彎菌門[3％(1 785/55 480)].在豐度方麵,伴根尖週炎組群落中的放線菌門顯著高于無根尖週炎組(P＜0.01).在檢齣率方麵,放線菌在伴根尖週炎組和無根尖週炎組中分彆是100％和50％.主成分分析結果錶明各樣本間的差異較大,但樣本有根據病變狀況(伴根尖週炎或無根尖週炎)聚類的趨勢.結論 根管治療後伴根尖週炎患牙的根管內微生物群落構成多樣性較高,與無根尖週炎患牙在根管內微生物群落構成上有一定差異.可能存在某種根管內群落構成模式與持續性根尖週感染具有一定的相關性.放線菌與根管治療後持續性根尖週感染可能具有一定的相關性.
목적 채용고통량초린산측서기술비교근관치료후반혹불반근첨주염환아근관내미생물군락적구성차이,심조여지속성근첨주감염상관적근관내가의미생물.방법 림상채집근관치료후반근첨주염환아(반근첨주염조,10과)화무근첨주염환아(무근첨주염조,10과)근관내미생물군락양본.제취양본중세균적총DNA,PCR확증기대응우16S rRNA편단상적V1～ V3고변구기인편단이구건굉기인조문고.경초린산측서후진행충계발육분석、다양성분석화주성분분석(PcoA분석).결과 공검출132충세균,분속우21개균문화96개균속.재문수평,반근첨주염조군락적주요균문위후벽균문[32％(18 534/58 688)]、변형균문[27％(15 626/58 688)]、방선균문[15％(8 685/58 688)]、의간균문[11％(6 163/58 688)]、사간균문[8％(4 761/58 688)]화라선체문[3％(1 785/58 688)].무근첨주염조군락적주요균문위후벽균문[31％(16 941/55 480)]、변형균문[27％(14 748/55 480)]、의간균문[18％(9 948/55 480)]、사간균문[10％(5 307/55 480)]、방선균문[9％(4 761/55 480)]화록만균문[3％(1 785/55 480)].재봉도방면,반근첨주염조군락중적방선균문현저고우무근첨주염조(P＜0.01).재검출솔방면,방선균재반근첨주염조화무근첨주염조중분별시100％화50％.주성분분석결과표명각양본간적차이교대,단양본유근거병변상황(반근첨주염혹무근첨주염)취류적추세.결론 근관치료후반근첨주염환아적근관내미생물군락구성다양성교고,여무근첨주염환아재근관내미생물군락구성상유일정차이.가능존재모충근관내군락구성모식여지속성근첨주감염구유일정적상관성.방선균여근관치료후지속성근첨주감염가능구유일정적상관성.
Objective To compare the intraradicular bacterial community structures of teeth with or without post-treatment periapical periodontitis and to explore the suspicious microorganisms that is related to persistent periapical infection.Methods The intraradicular biofilm samples were collected from 10 post-treatment periapical periodontitis teeth(apical periodontitis group) and 10 teeth without post-treatment periapical periodontitis(without apical periodontitis group).The V 1-V3 hypervariable regions of bacterial 16S rRNA genes were amplified,and the high-throughput pyrosequencing was performed.The composition and structure characteristic of intraradicular microbiome were revealed by bioinformatic analysis.Results Total sequences were taxonomically classified into 132 species-level bacteria belonging to 96 genera and 21 phyla.The most representive phyla in apical periodontitis group were Firmicutes[32％ (18 534/58 688)],Proteobacteria[27％(15 626/58 688)],Actinobacteria[15％ (8 685/58 688)],Bacteroidetes [11％ (6163/58 688)],Fusobacteria[8％ (4761/58688)] and Spirochaetes[3％(1 785/58 688)].While the most representive phyla in without apical periodontitis group were Firmicutes[31％(16 941/55 480)],Proteobacteria [27％(14 748/55 480)],Bacteroidetes [18％(9 948/55 480)],Fusobacteria[10％(5 307/55 480)],Actinobacteria [9％(4 761/55 480)],Chloroflexi[3％(1 785/55 480)].The abundance of actinobacteria in apical periodontitis group was significantly higher than without apical periodontitis group(P＜0.01).The detection rates of actinomycetes in apical periodontitis group and without apical periodontitis group were 100％ and 50％.Conclusions The diversity of intraradicular bacterial community in teeth with apical periodontitis was higher than those without apical periodontitis.Actinomycetes may be related to post-treatment periapical periodontitis.
