CAJ | 학술논문

目的评估黄芩甙元和槲皮黄酮对牙本质胶原耐酶解能力的影响,为寻找理想的牙本质粘接预处理剂提供实验基础.方法黄芩甙元、槲皮黄酮及原花色素以20％二甲基亚砜乙醇溶液作为溶剂,配制成质量浓度为50 g/L的预处理剂,制备脱矿牙本质试件(黄芩甙元组、槲皮黄酮组及原花色素组,每组19个试件).37℃下预处理剂孵育24 h后,于酶解液中(含Ⅰ型胶原酶)进行消化.空白对照组和阴性对照组(每组19个试件)以20％二甲基亚砜乙醇溶液作为预处理剂,空白对照组所用酶解液不含Ⅰ型胶原酶.分别测试酶解24 h后各组试件的极限拉伸强度(每组10个试件)及酶解液羟脯氨酸含量(每组6个试件),场发射扫描电镜观察酶解12h后牙本质胶原形貌(每组3个试件).结果酶解24 h后,黄芩甙元组极限拉伸强度最高[(16.00±1.31) MPa],其次为原花色素组[(12.64±0.91) MPa]、空白对照组[(7.84±1.18) MPa]、槲皮黄酮组[(3.20±1.07) MPa]、阴性对照组(0 MPa),各组间差异均有统计学意义(P＜0.01).空白对照组酶解液羟脯氨酸含量最低[(0.40±0.16) mg/L],其次为黄芩甙元组[(2.95±0.18) mg/L]、原花色素组[(4.78±0.38 mg/L]、槲皮黄酮组[(28.22± 1.53) mg/L]、阴性对照组[(34.39±0.39) mg/L],各组间差异均有统计学意义(P＜0.01).酶解12h后场发射扫描电镜显示空白对照组牙本质胶原网络完整;阴性对照组胶原网络完全断裂塌陷;槲皮黄酮组大部分胶原被降解;原花色素组仅少量胶原断裂塌陷;黄芩甙元组胶原网络基本维持完整.结论 50 g/L黄芩甙元和槲皮黄酮均可提高牙本质胶原的耐酶解能力,其中黄芩甙元作用优于原花色素,槲皮黄酮作用弱于原花色素.
목적 평고황금대원화곡피황동대아본질효원내매해능력적영향,위심조이상적아본질점접예처리제제공실험기출.방법 황금대원、곡피황동급원화색소이20％이갑기아풍을순용액작위용제,배제성질량농도위50 g/L적예처리제,제비탈광아본질시건(황금대원조、곡피황동조급원화색소조,매조19개시건).37℃하예처리제부육24 h후,우매해액중(함Ⅰ형효원매)진행소화.공백대조조화음성대조조(매조19개시건)이20％이갑기아풍을순용액작위예처리제,공백대조조소용매해액불함Ⅰ형효원매.분별측시매해24 h후각조시건적겁한랍신강도(매조10개시건)급매해액간포안산함량(매조6개시건),장발사소묘전경관찰매해12h후아본질효원형모(매조3개시건).결과 매해24 h후,황금대원조겁한랍신강도최고[(16.00±1.31) MPa],기차위원화색소조[(12.64±0.91) MPa]、공백대조조[(7.84±1.18) MPa]、곡피황동조[(3.20±1.07) MPa]、음성대조조(0 MPa),각조간차이균유통계학의의(P＜0.01).공백대조조매해액간포안산함량최저[(0.40±0.16) mg/L],기차위황금대원조[(2.95±0.18) mg/L]、원화색소조[(4.78±0.38 mg/L]、곡피황동조[(28.22± 1.53) mg/L]、음성대조조[(34.39±0.39) mg/L],각조간차이균유통계학의의(P＜0.01).매해12h후장발사소묘전경현시공백대조조아본질효원망락완정;음성대조조효원망락완전단렬탑함;곡피황동조대부분효원피강해;원화색소조부소량효원단렬탑함;황금대원조효원망락기본유지완정.결론 50 g/L황금대원화곡피황동균가제고아본질효원적내매해능력,기중황금대원작용우우원화색소,곡피황동작용약우원화색소.
Objective To investigate the effect of baicalein and quercetin on the enzymatic resistance of dentin matrix collagen.Methods Baicalein,quercetin and proanthocyanidin were dissolved in 20％ dimethyl sulfoxide (DMSO) ethanol and prepared into pretreatment agents with a concentration of 50 g/L.Demineralized dentin specimens were prepared and immersed in pretreatment agents at 37 ℃ for 24 h,then they were digested in solution containing type Ⅰ collagenase.The pretreatment agents of blank control group and negative control group were 20％ DMSO ethanol,blank control group were digested in solution without collagenase.The ultimate tensile strength (UTS) and the hydroxyproline content of enzymolysis liquid in each group were measured respectively after collagenase digestion for 24 h,the dentin collagen morphology were observed under a field emission scanning electron microscopic (FE-SEM) after collagenase digestion for 12 h.Results After collagenase digestion for 24 h,the baicalein group had the highest UTS [(16.00 ± 1.31) MPa],followed by proanthocyanidin group [(12.64 ± 0.91) MPa],blank control group [(7.84± 1.18) MPa],quercetin group [(3.20± 1.07) MPa],and negative control group (0 MPa).Significant differences were detected among the UTS in each two group (P＜0.01).The hydroxyproline content in blank control group was the lowest [(0.40 ± 0.16) mg/L],followed by baicalein group[(2.95 ± 0.18) mg/L],proanthocyanidin group [(4.78±0.38) mg/L],quercetin group[(28.22± 1.53) mg/L],and negative control group [(34.39±0.39) mg/L].There were significant differences among the hydroxyproline contents in each group (P＜ 0.01).After collagenase digestion for 12 h,intact collagen network could be seen in blank control group under a FE-SEM.Collagen network in negative control group suffered nearly complete destruction and collapsed.In quercetin group,most of collagen collapsed.In proanthocyanidin group,a small portion of collagen destruction and collapse could be seen.In baicalein group,collagen network remained intact.Conclusions The use of baicalein and quercetin could improve enzymatic resistance of dentin matrix collagen at a concentration of 50 g/L.The effect of baicalein was better than that of proanthocyanidin while the effect of quercetin was weaker than that of proanthocyanidin.