中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2014年
11期
688-692
,共5页
王丹杨%张凌%李芳%马克强%陈吉华
王丹楊%張凌%李芳%馬剋彊%陳吉華
왕단양%장릉%리방%마극강%진길화
基质金属蛋白酶类%牙本质%免疫组织化学
基質金屬蛋白酶類%牙本質%免疫組織化學
기질금속단백매류%아본질%면역조직화학
Matrix metalloproteinases%Dentin%Immunohistochemistry
目的 比较5种基质金属蛋白酶(matrix metalloproteinase,MMP)在人冠方牙本质中的分布情况,以期为与胶原降解相关的各种牙体硬组织疾病研究提供科学基础.方法 将10颗磨牙制成牙本质组织片60片,分为5组,每组再分成2个亚组(每亚组6片),分别采用化学染色法和荧光染色法进行免疫组化染色,光学和荧光显微镜观察.将27颗磨牙纵切成牙本质片,制备浅层和深层牙本质粉末,借助液相芯片技术分别检测MMP-1、2、3、8、9含量.结果 5种MMP在牙本质中均呈阳性表达,深层牙本质染色更深,釉质牙本质界内侧存在MMP-2、8、9高表达条带.牙本质浅层和深层MMP-1、2、3、8、9含量分别为(0.037±0.025)和(0.433±0.089)、(0.445±0.115)和(2.730±0.712)、(0.071±0.069)和(0.460±0.108)、(0.586±0.246)和(6.159±0.948)、(0.384±0.185)和(1.460±0.251) ng/mg.深层牙本质中每种MMP含量均显著高于浅层(P＜0.05).结论 人冠方牙本质含MMP-1、2、3、8和9,其分布规律为从牙本质深层至浅层含量逐渐降低.
目的 比較5種基質金屬蛋白酶(matrix metalloproteinase,MMP)在人冠方牙本質中的分佈情況,以期為與膠原降解相關的各種牙體硬組織疾病研究提供科學基礎.方法 將10顆磨牙製成牙本質組織片60片,分為5組,每組再分成2箇亞組(每亞組6片),分彆採用化學染色法和熒光染色法進行免疫組化染色,光學和熒光顯微鏡觀察.將27顆磨牙縱切成牙本質片,製備淺層和深層牙本質粉末,藉助液相芯片技術分彆檢測MMP-1、2、3、8、9含量.結果 5種MMP在牙本質中均呈暘性錶達,深層牙本質染色更深,釉質牙本質界內側存在MMP-2、8、9高錶達條帶.牙本質淺層和深層MMP-1、2、3、8、9含量分彆為(0.037±0.025)和(0.433±0.089)、(0.445±0.115)和(2.730±0.712)、(0.071±0.069)和(0.460±0.108)、(0.586±0.246)和(6.159±0.948)、(0.384±0.185)和(1.460±0.251) ng/mg.深層牙本質中每種MMP含量均顯著高于淺層(P＜0.05).結論 人冠方牙本質含MMP-1、2、3、8和9,其分佈規律為從牙本質深層至淺層含量逐漸降低.
목적 비교5충기질금속단백매(matrix metalloproteinase,MMP)재인관방아본질중적분포정황,이기위여효원강해상관적각충아체경조직질병연구제공과학기출.방법 장10과마아제성아본질조직편60편,분위5조,매조재분성2개아조(매아조6편),분별채용화학염색법화형광염색법진행면역조화염색,광학화형광현미경관찰.장27과마아종절성아본질편,제비천층화심층아본질분말,차조액상심편기술분별검측MMP-1、2、3、8、9함량.결과 5충MMP재아본질중균정양성표체,심층아본질염색경심,유질아본질계내측존재MMP-2、8、9고표체조대.아본질천층화심층MMP-1、2、3、8、9함량분별위(0.037±0.025)화(0.433±0.089)、(0.445±0.115)화(2.730±0.712)、(0.071±0.069)화(0.460±0.108)、(0.586±0.246)화(6.159±0.948)、(0.384±0.185)화(1.460±0.251) ng/mg.심층아본질중매충MMP함량균현저고우천층(P＜0.05).결론 인관방아본질함MMP-1、2、3、8화9,기분포규률위종아본질심층지천층함량축점강저.
Objective To compare the distribution and concentrations of matrix metalloproteinase (MMP)-1,2,3,8,9 in human coronal dentin.Methods The localization of five types of MMP was performed using immunohistochemistry.Molars were demineralized and sectioned into 5 μm thick specimens.All specimens were randomly divided into five groups according to the antibodies.Each group contained two subgroups (n=6).Immunoreactivity of each subgroup was visualized with 3,3-diaminobenzidine solution or fluorescein isothiocyanate and observed under microscopy respectively.Molars were sectioned into slices.The slices were divided into two groups according to superficial or deep dentin and pulverized to fine powder.After dentin protein was extracted,the concentrations of MMP-1,2,3,8,9 were detected by using fluorescent microsphere immunoassay.Results Immunohistochemical staining revealed that MMP-1,2,3,8,9 were highly concentrated in the deep dentin.However,intense immunoreactivities of MMP-2,8,9 were identified in a 6-10 μm wide zone adjacent to the dentino-enamel junction.The content of MMP-1 in superficial layer and deep layer of dentin were (0.037 ± 0.025) and (0.433 ± 0.089) ng/mg.The content of MMP-2 in superficial layer and deep layer of dentin were (0.445 ±0.115) and (2.730±0.712) ng/mg.The content of MMP-3 in superficial layer and deep layer of dentin were (0.071 ±0.069) and (0.460±0.108) ng/mg.The content of MMP-8 in superficial layer and deep layer of dentin were (0.586±0.246) and (6.159±0.948) ng/mg.The content of MMP-9 in superficial layer and deep layer of dentin were (0.384±0.185) and (1.460± 0.251) ng/mg.The concentrations of all tested MMP were significantly higher in deep dentin than those in superficial dentin (P＜0.05).Conclusions There are five types of MMP contained in human coronal dentin,and the distribution of MMP shows a decreasing trend from the deep dentin to the superficial dentin.
